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   细胞贮存 在 生物学 分类中 的翻译结果: 查询用时:0.584秒
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细胞贮存
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  “细胞贮存”译为未确定词的双语例句
    V 79 cells treated with potassium dichromate and control cells were stored at 4℃, -20℃,-80℃ and -196℃. Single cell gel electrophoresis (SCGE) was carried out after preserving cells for 0, 4, 24, 48 and 168 hours.
    将未处理的(对照)和重铬酸钾处理的V79细胞贮存于4℃、-20℃、-80℃和-196℃,于保存后的0,4,24,48,168h取出进行单细胞凝胶电泳(SCGE)试验。
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    Many factors such as: temperature, pH, store time, some reagents and pathological changes etc. can influence the distribution of PTPP activities between cytosol and membrane.
    很多因素诸如:病变、PH值、温度、离子强度、细胞贮存时间以及药物等,对PTPP在胞浆与膜中的分布有影响。
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    The characteristic structure is that the epithelial cells in the middle tubule are formed by R cells and B cells during the second development of the ovary. The R cells stocked fat and B cells formed transferred vacuoles. The nutrition storing in hepotopancreas were transferred into the lumen by the vacuole in B-cells during the second development of the ovary.
    二次卵巢发育时肝小管的主要结构特征为:在肝小管中部,几乎都是由R细胞和B细胞组成,表现为肝胰腺活跃的组织特征,R细胞贮存了丰富的脂肪,成熟B细胞转运泡释放入管腔.
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  storage of cells
Such interpretation is extended to the storage of cells immobilized in Ca2+-alginate gel.
      
In conclusion, the development of solutions designed for low-temperature storage of cells provides a viable alternative to more conventional cryopreservation protocols and eliminates the necessity of including serum in these formulations.
      
The attachment and growth properties are stable after storage of cells in liquid nitrogen.
      
Granulocytes were fixed to preserve morphology and allow storage of cells on slides.
      


Through assaying the protein tyrosine phosphatases(PTPPs) activities of cytosol and membrane of human erythrocytes, we found that there were 70-80% PTPP activities of normal red blood cell in the cytosol, and only 20-30% PTPP activities in the membrane.Many factors such as: temperature, pH, store time, some reagents and pathological changes etc. can influence the distribution of PTPP activities between cytosol and membrane. These suggest that PTPP of membrane may be released and enter into cytosol through a...

Through assaying the protein tyrosine phosphatases(PTPPs) activities of cytosol and membrane of human erythrocytes, we found that there were 70-80% PTPP activities of normal red blood cell in the cytosol, and only 20-30% PTPP activities in the membrane.Many factors such as: temperature, pH, store time, some reagents and pathological changes etc. can influence the distribution of PTPP activities between cytosol and membrane. These suggest that PTPP of membrane may be released and enter into cytosol through a certain mechanism; On the other hand, the PTPP of cytosol may also be associated with membrane.

通过测定红细胞胞浆及膜中的PTPP活性,发现人正常血红细胞中胞浆PTPP活性约占红细胞PTPP总活性的70-80%,膜中只有约20-30%的PTPP活性。很多因素诸如:病变、PH值、温度、离子强度、细胞贮存时间以及药物等,对PTPP在胞浆与膜中的分布有影响。可以推测:膜上的PTPP能通过某种机制解离下来,进入胞浆;相反的过程,胞浆中的PTPP也能通过某种机制与膜结合。这种偶联与去偶联的具体机制及其生理功能还有待进一步探索。

V 79 cells treated with potassium dichromate and control cells were stored at 4℃, -20℃,-80℃ and -196℃. Single cell gel electrophoresis (SCGE) was carried out after preserving cells for 0, 4, 24, 48 and 168 hours. The results showed that there was no difference on DNA migration between cells processing immediately after trypsinization and cells preserving for 24h at different low temperatures. Longer storage (48h or 168h) resulted in a significant increase in the length of DNA migration and decrease in cell...

V 79 cells treated with potassium dichromate and control cells were stored at 4℃, -20℃,-80℃ and -196℃. Single cell gel electrophoresis (SCGE) was carried out after preserving cells for 0, 4, 24, 48 and 168 hours. The results showed that there was no difference on DNA migration between cells processing immediately after trypsinization and cells preserving for 24h at different low temperatures. Longer storage (48h or 168h) resulted in a significant increase in the length of DNA migration and decrease in cell viability. The results suggested that 4℃ is more convenient for less than 24h storage without influence on DNA migration and -80℃ is better for longer storage to minimize the influence on DNA migration.

将未处理的(对照)和重铬酸钾处理的V79细胞贮存于4℃、-20℃、-80℃和-196℃,于保存后的0,4,24,48,168h取出进行单细胞凝胶电泳(SCGE)试验。结果显示各温度保存24h的细胞DNA迁移长度与新鲜细胞无差异;更长时间(48和168h)的保存细胞DNA迁移长度明显增加;同时各温度保存的细胞存活率均随贮存时间的延长而下降。因此,24h内细胞保存以4℃为好,而24h以上的保存则以-80℃为佳

Seeds of Eleutherococcus brachypus Harms were flat kidney shaped and their seed coats were only composed of one layer of cells. Embryos with abundant protein in their cells were just at the heart shaped stage and were capped by sacs formed from degenerating endosperm cells when seeds shed from their maternal plants. A large amount of stored protein grains and lipids existed in endosperm cells but no polysaccharide grains were present either in endosperm cells or in embryo cells. Viable seeds were only 9.27%...

Seeds of Eleutherococcus brachypus Harms were flat kidney shaped and their seed coats were only composed of one layer of cells. Embryos with abundant protein in their cells were just at the heart shaped stage and were capped by sacs formed from degenerating endosperm cells when seeds shed from their maternal plants. A large amount of stored protein grains and lipids existed in endosperm cells but no polysaccharide grains were present either in endosperm cells or in embryo cells. Viable seeds were only 9.27% of the total. The plump seeds germinated in the cultivated field after 18~19 months and their germinating rate was 1.67%. Besides, the content of protein decreased gradually and a few polysaccharide grains were stored in embryo cells during the process. The afterripening process of seeds stratified at different temperatures ended after 6 months and the cytochemistry features of the seeds were that the content of protein decreased gradually and numerous polysaccharide grains had been stored in embryo cells at the late heart shaped embryo stage and retained till the mature embryo stage. The structure, afterripening and cytochemistry of seeds were compared between Eleutherococcus brachypus and Eleutherococcus senticosus. The poor quality of the seeds, longer time of afterripening in a natural state and much lower germination rate of E. brachypus are considered to be important reasons for the endangerment of this species. Some measures are suggested for its conservation based on the above facts.

短柄五加EleutherococusbrachypusHarms种子为扁肾形,种皮由一层细胞构成。种子脱落时,胚为心形胚期,胚周围的胚乳细胞解体形成液样囊腔,并包裹胚,胚细胞中存在较多蛋白质,胚乳细胞贮存大量蛋白质颗粒和脂类,但两者均未见多糖颗粒。有萌发潜能的种子只占全部种子的9.27%。在试验地种植条件下饱满种子经18~19个月后萌发,出苗率为1.67%,该过程细胞化学特点是胚细胞中蛋白质含量逐渐减少,并逐渐积累少量多糖颗粒。种子经变温层积处理6个月即可完成后熟过程,其细胞化学特点是胚细胞中蛋白质含量逐渐减少,但在心形胚后期即已积累大量多糖颗粒,并一直保持至胚完全发育成熟。经比较短柄五加与刺五加的种子结构、后熟作用及其细胞化学特点,认为短柄五加种子质量差、自然状态下后熟时间长和出苗率低是短柄五加致濒的重要生殖因素,并提出了相应保护方法。

 
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