助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   细胞解冻 的翻译结果: 查询用时:0.009秒
图标索引 在分类学科中查询
所有学科
材料科学
更多类别查询

图标索引 历史查询
 

细胞解冻
相关语句
  “细胞解冻”译为未确定词的双语例句
     2. At 48h after thawed and cultured on MEF feeder layer in complete medium supplied with LIF (1000U/ml), mouse ES cells formed clones with clear edge, smooth surface. The cell boundary was disappeared within clones and no differentiated cells were found in the edge of clones.
     2.小鼠ES细胞解冻后,在饲养层培养体系用含LIF(1000U/ml)的完全培养液培养48h时,ES细胞形成边界清晰光滑的克隆,克隆内细胞排列紧密,难以辩认单个细胞,克隆边缘未见分化细胞;
短句来源
     Objective To evaluate the effects of human frozen thawed decidua monolayer cells on mouse embryo development in a co culture system.
     目的 探讨冻融的人早孕蜕膜单层细胞对小鼠胚胎发育的影响。 方法 蜕膜单层细胞解冻后接种获得冻融蜕膜单层细胞;
短句来源
     Fetal calf serum (FCS) , penicillin, and streptomycin were from Flow Laboratories Inc. Insulin (activity 26. 3 U/mg) was purchased from Calbiochems.
     将冻结的外周血 CD34”细胞解冻,悬浮于 IMDM(含30%FCS和 100U/rnl DNA酶)中,然后在4009,4℃下高速离心smin,去上清,洗细胞二次,再悬浮于含 0.3%去离子 BSA的 IMDM中。
短句来源
     We tested the survival and increase of BMSc growth by the relative data from MTT method,detected the rate of ALP(+) by the improved Gomori' s method.
     法检测细胞增长和存活情况,采用改良Gomori钙钻法计数细胞碱性磷酸酶(ALp)阳性率。 冻存细胞解冻后,接种培养,观察其增长和分化情况。
短句来源
     Results: 1. Viability of fresh porcine hepatocytes was 81%+3.6%, While Gradually freezing group had a viability of 76.3%+1.9% and programmed freezing group's viability was 72.4%+1.5%.
     梯度降温组细胞解冻后的活率为 76.3%士 1.9%; 程控降温组细胞解冻后的活率为 72.4%土 1.5%。
短句来源
更多       
  相似匹配句对
     2. basal cell;
     基细胞;
短句来源
     Tom Clancys Splinter Cell
     细胞分裂
短句来源
     Moreover, there was 70-80% of cells survived after frozen and thawed.
     细胞冷冻保存解冻后复苏率达70%~80%;
短句来源
     Thawing was in a water bath at 35 ℃.
     35℃水浴解冻
短句来源
     Thawing was in 35℃ water bath.
     35℃水浴解冻
短句来源
查询“细胞解冻”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
没有找到相关例句


Factors affecting oocytes in vitro maturation(IVM)from different size of follicles and cryopreservation of oocytes in different development phases were studied.The results showed that the maturation rate of IVM was very significantly higher in the medium containing 10%ECS(72 86%)than that containing 10%NCS(62 21%)(P<0 05).Addition of 10% porcine follicular fluid(pFF)to mature medium inhibited the in vitro maturation of oocytes.Oocytes from different size of follicles had different development ability.Oocytes...

Factors affecting oocytes in vitro maturation(IVM)from different size of follicles and cryopreservation of oocytes in different development phases were studied.The results showed that the maturation rate of IVM was very significantly higher in the medium containing 10%ECS(72 86%)than that containing 10%NCS(62 21%)(P<0 05).Addition of 10% porcine follicular fluid(pFF)to mature medium inhibited the in vitro maturation of oocytes.Oocytes from different size of follicles had different development ability.Oocytes collected from big(>5 mm)and medium(2?5 mm)follicles had a higher maturation rate than oocytes from small(<2 mm)follicle (P<0 05).Oocytes in MⅡ stage had higher antifreezing ability than GV stage and oocytes culture for 24 h.The survival rate of oocytes after cryopreservation and thawing was 35 59%,significant higher than oocytes cryopreservation in GV stages(24 64%)and culture for 24 h(23 36%)(P<0 05).The rate of morphological normality after thawing was significantly lower in group of GV stages(53 24%)than in group of MⅡ stage(72 81%)and culture for 24 h(77 22%)(P<0 05).

比较从不同卵巢卵泡直径获得的卵母细胞体外成熟能力的差异 ,并研究其冷冻保存效果。结果表明 :在体外成熟培养液中添加 10 % (体积分数 ,下同 )ECS的成熟率 (72 86 % )显著高于添加 10 %NCS (6 2 2 1% ) (P <0 0 5 ) ,而添加10 %pFF则抑制卵母细胞的体外成熟。随着卵泡直径的增大 ,卵母细胞体外成熟能力逐渐增强。采用程序冷冻保存方法 ,培养成熟组的卵母细胞成活率 (35 5 9% )显著高于培养前 (2 4 6 4 % )和培养 2 4h组 (2 3 36 % ) (P <0 0 5 )。培养 2 4h(77 2 2 % )和培养成熟 (72 81% )组 ,卵母细胞解冻后的形态完整率均显著高于培养前 (5 3 2 4 % ) (P <0 0 5 )。

Goat fetal fibroblasts were frozen at different tem perature with various protecting solution in order to find a proper procedure to preserve its rare resources for further study both in transgenic and cloning practice. The result showed that fetal fibroblasts froze at- 196℃ with 90 0 m L/ L fetal bovine serum(FBS) grew faster than that at- 80℃ after thawing(P<0 .0 5 ) .- 2 0℃ was not an effective temperature in frozen cells.10 0 m L/ L dimethyl sulfoxide(DMSO) contained in frozen solution was better than...

Goat fetal fibroblasts were frozen at different tem perature with various protecting solution in order to find a proper procedure to preserve its rare resources for further study both in transgenic and cloning practice. The result showed that fetal fibroblasts froze at- 196℃ with 90 0 m L/ L fetal bovine serum(FBS) grew faster than that at- 80℃ after thawing(P<0 .0 5 ) .- 2 0℃ was not an effective temperature in frozen cells.10 0 m L/ L dimethyl sulfoxide(DMSO) contained in frozen solution was better than 5 0 m L/ L in im proving protection effec- tiveness(P<0 .0 5 ) .In vitro culture of fetal fibroblasts experiment showed that both DMEM and M199can sup- port cell growth and proliferation and that a supplem entation of 2 mm ol/ L 2 - m ercaptoethanol (2 - Me) was better than not in improving tissue vitality and growth speed.In cell culture procedure,2 - Me could improve the effect of 10 0 m L / L FBS in stim ulating cell proliferation.And individual 2 - Me supplementation in the culture medium could stimulate cell growth.As a result,2 - Me was more likely a cell proliferation stim ulator than an oxidation retarding factor.High serum concentration10 0 m L/ L was vital for cell proliferation,serum starvation(5 m L/ L FBS) could not only inhibit cell division but also cause cell loss during long term culture.

将胎儿成纤维细胞悬浮于不同的冷冻保护液中 ,在不同的温度下进行冷冻保存试验。结果显示 ,当保护液中含有 90 0 m L / L胎牛血清 (FBS)时 ,- 196℃下保存的山羊胎儿成纤维细胞解冻后的细胞贴壁率高于- 80℃ ,且前者贴壁细胞增殖也较后者快 ,二者间差异显著 ;而在 - 2 0℃条件下只有一小部分细胞能够存活 ,与前两者相比差异极显著 ,说明 - 2 0℃不适合冷冻胎儿细胞。冷冻保护液中含有 10 0 m L / L二甲基亚砜 (DMSO)的保护效果明显好于 5 0 m L / L DMSO,二者间差异显著。用组织块法培养的细胞 ,在两种培养基 DMEM和 M199中添加 10 0 m L / L FBS和 2 mm ol/ L 2 -巯基乙醇 (2 - Me) ,其组织块成活率及细胞生长速度均高于单纯添加 10 0 m L / LFBS,同时证明 DMEM和 M199都能用于胎儿成纤维细胞的体外培养。以 DMEM培养液为基础 ,分别添加 2mm ol/ L 2 - Me,5 m L / L FBS,10 0 m L / L FBS和 2 m mol/ L 2 - Me+10 0 ...

将胎儿成纤维细胞悬浮于不同的冷冻保护液中 ,在不同的温度下进行冷冻保存试验。结果显示 ,当保护液中含有 90 0 m L / L胎牛血清 (FBS)时 ,- 196℃下保存的山羊胎儿成纤维细胞解冻后的细胞贴壁率高于- 80℃ ,且前者贴壁细胞增殖也较后者快 ,二者间差异显著 ;而在 - 2 0℃条件下只有一小部分细胞能够存活 ,与前两者相比差异极显著 ,说明 - 2 0℃不适合冷冻胎儿细胞。冷冻保护液中含有 10 0 m L / L二甲基亚砜 (DMSO)的保护效果明显好于 5 0 m L / L DMSO,二者间差异显著。用组织块法培养的细胞 ,在两种培养基 DMEM和 M199中添加 10 0 m L / L FBS和 2 mm ol/ L 2 -巯基乙醇 (2 - Me) ,其组织块成活率及细胞生长速度均高于单纯添加 10 0 m L / LFBS,同时证明 DMEM和 M199都能用于胎儿成纤维细胞的体外培养。以 DMEM培养液为基础 ,分别添加 2mm ol/ L 2 - Me,5 m L / L FBS,10 0 m L / L FBS和 2 m mol/ L 2 - Me+10 0 m L / L FBS对山羊胎儿成纤维细胞进行体外培养 ,结果显示 ,细胞在 10 0 m L / L FBS和 2 mm ol/ L 2 - Me+10 0 m L / L FBS培养液中生长迅速 ,两者间无明显差异 ,2 - Me的存在促进了细胞的增殖 ;两者都与添加 2 m mol/ L 2 - Me组存在显著差异 ;添加 2 m mol/ L 2 - Me和 5m L / L FBS相

Objective To explore vitrified method and cryopreservative solution of mouse oocytes. Method The oocytes of ICR and C 57 BL/6J mice were vitrified with EFS40(high concentration of sodium) and DEFS40(low concentration of sodium),thawed with 0.5mol/L sugar solution.Before fertilization in vitro, zona-pellucieda of C 57 BL/6J mice thawed oocytes were dissected. Results Considering the vitrified effect, DEFS40 is better than EFS40. The oocyte survival rate of ICR mice reaches 75.2% after thawed. The fertilization...

Objective To explore vitrified method and cryopreservative solution of mouse oocytes. Method The oocytes of ICR and C 57 BL/6J mice were vitrified with EFS40(high concentration of sodium) and DEFS40(low concentration of sodium),thawed with 0.5mol/L sugar solution.Before fertilization in vitro, zona-pellucieda of C 57 BL/6J mice thawed oocytes were dissected. Results Considering the vitrified effect, DEFS40 is better than EFS40. The oocyte survival rate of ICR mice reaches 75.2% after thawed. The fertilization rate reaches 24.6% and the difference is not remarkable compared to the control.And the oocyte survival rate of C 57 BL/6J mice reaches 87.1% after thawed. The oocyte fertilized rate which zona-pellucieda were dissected reaches 46.0% and the difference is not remarkable compared to the control.Conclusion The vitrified effect of mice oocyte is influenced by the concentration of sodium.The perfect effect could be obtained when the oocytes were vitrified with DEFS40.

目的 对小鼠卵母细胞玻璃化的方法和冷冻液进行研究。方法 用两种玻璃化冷冻液EFS4 0 (高钠 )、DEFS4 0 (低钠 )对ICR、C57BL 6J小鼠卵母细胞进行冷冻 ,0 5mol L蔗糖液解冻 ,体外授精前对部分解冻后的C57BL 6J小鼠卵母细胞进行透明带切割。结果 DEFS4 0冷冻液冷冻效果明显高于EFS4 0冷冻液 ,ICR小鼠卵母细胞解冻后成活率达 75 2 %、体外授精率 (2 4 6 % )与对照组相比差异无显著性 ,C57BL 6J小鼠卵母细胞解冻后成活率为 87 1%、卵母细胞切割后体外受精率 (36 0 % )与对照组相比差异也无显著性。结论 冷冻液中钠离子浓度影响小鼠卵母细胞冷冻效果 ,用DEFS4 0 (低钠 )冷冻液进行玻璃化冷冻可以取得理想的冷冻效果。

 
<< 更多相关文摘    
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关细胞解冻的内容
在知识搜索中查有关细胞解冻的内容
在数字搜索中查有关细胞解冻的内容
在概念知识元中查有关细胞解冻的内容
在学术趋势中查有关细胞解冻的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社