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   细胞酶 的翻译结果: 查询用时:1.029秒
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细胞酶
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  cellular enzyme
     The RASF proliferation was determined by 3H-TdR incorporation, and the productions of PGE2 and NO in culture supernatants of RASF were detected with competitive ELISA and enzyme reduction of nitrate. Expressions of COX-2 and iNOS mRNA in RASF were analyzed by semi-quantitative RT-PCR. Expressions of COX-2 and iNOS protein were estimated by Western-blot method and cellular enzyme immunoassay in synovial fibroblasts.
     分别用 3 H-Td R法、竞争酶联免疫吸附试验 (ELISA)和硝酸酶还原法、半定量逆转录 -聚合酶联反应 (RT- PCR)法、细胞酶免疫法及蛋白免疫印迹 (Western- blot)法 ,检测滑膜细胞的增殖活性、细胞培养上清液中 PGE2和 NO的水平 ,滑膜细胞 COX- 2和 i NOSm RNA表达水平及蛋白表达水平。
短句来源
  cell-enzyme
     A Study on Preparation of FDP with Cell-enzyme
     细胞酶制备二磷酸果糖(FDP)的研究
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  cell enzyme
     Add RPMI1640 completed culture medium containing 10% FCS to culture for 48hs continously twelve hours after transfectin instantly the cervix cancer U14 cells cultured in suspension, and then compare and observe the p53 expression through indirect cell enzyme immunol chemistral method.
     体外瞬时转染悬浮培养的宫颈癌U14细胞12h后加入含10%胎牛血清的RPMI 1640完全培养基继续培养48h,然后用间接细胞酶免疫化学法比较观察p53蛋白的表达。
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     Evaluating of the activity of rabbit corneal endothelial cell enzyme through enzyme histochemistry and electron microscope histochemical technics to determine how much the VFDP influences the ability of rabbit cornea endothelial cell.
     利用光镜、电镜酶组织化学方法可测定兔角膜内皮细胞酶活性。 本实验研究旨在通过光镜、电镜酶组织化学方法对冻干保存的兔角膜内皮细胞的三磷酸腺苷酶(ATPase)及琥珀酸脱氢酶(SDH)进行检测,从而判定真空冷冻干燥保存法(冻干法)对于兔角膜内皮细胞活性的影响。
短句来源
     Methods:AEAC were detected in serum samples of 41 patients with AS using cell enzyme linked immunosorbent assay(C ELISA); the CMV(IgG)was analyzed by indirect ELISA.
     方法 应用细胞酶联免疫反应 (C -ELISA)和ELISA技术检测 41例冠状动脉粥样硬化患者血清中AECA和抗HCMV抗体 (IgG)。
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     The whole cell enzyme activity was characterized with 4-nitrophenyl ramifications used as the substrate. Results show that the optimal substrate is 4-nitrophenylcaprate(C_(10)).
     以不同碳链长度的对硝基苯酚酯为底物对全细胞酶活进行性质检测,结果表明:对硝基苯酚癸酸酯(C10)为最适反应底物;
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  “细胞酶”译为未确定词的双语例句
     . 8. The activity oftrehalose synthase was increased by addition of Mg2~, K~ and Na~, but was inhibited by2+ 2+ 2+ 2+ 2+Zn , Mn , Cu , Ca Ba .
     (3)Mg~(2+)及K~+对该细胞酶有明显激活作用,Na~+稍有激活作用,Ca~(2+),Ba~(2+)对该酶稍有抑制作用,而Zn~(2+),Mn~(2+)及Cu~(2+)则可强烈地抑制酶活力;
短句来源
     【Method】 Truncated 45W-4B with the signal peptide sequence deletion and C’-terminal nucleotides encoding,17 hydrophobic amino acids were cloned into BamH I and EcoR I pre-digested pGEX-4T-1 and transformed into competent BL21.The positive transformants were determined by enzyme restriction,PCR,and sequencing.
     【方法】PCR扩增截去45W-4B基因的信号肽和C端17个疏水氨基酸序列,经BamH I和EcoR I酶切后与表达载体pGEX-4T-1连接转化BL21感受态细胞,酶切及PCR扩增鉴定阳性克隆。
短句来源
     The interest fragments TSO18 and 45W-4BX were ligated with an expression vector pGEX-4T-l with the same cohesive ends, transformed into competent bacteria BL_21 and identified with enzyme restriction and PCR and sequencing.
     将TSO18和45W-4BX经限制性内切酶处理后与相同处理的表达载体pGEX-4T-1连接,转化BL_(21)感受态细胞,酶切及PCR扩增鉴定获得两基因的重组表达菌株。
短句来源
     The enzyme activity of immobilized cells was 81.99 u/g.
     制得的固定化细胞酶活力为 81.99u/ g.
短句来源
     The enzyme exhibited an optimumtemp erature of 35℃,and an optimumpHof7.0~7.8.The enzyme was stable at30~40℃,pH6.6~7.8.The activity of treh alose synthase was increased by addition of Mg 2+ ,K + and Na + ,but was inhibited by Zn 2+ ,Mn 2+ ,Cu 2+ ,Ca 2+ ,Ba 2+ .
     研究表明,该细胞酶的反应最适温度为35℃,最适pH7.4,Mg2+及K+对该细胞酶有明显激活作用,Zn2+,Mn2+及Cu2+则可强烈地抑制酶活力。
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  cellular enzyme
Serum-5'-N was not well correlated with cellular enzyme activity in the same patient and therefore appears less suitable as diagnostic marker enzyme.
      
Fusions between BB rat lymphocytes and rat myeloma cells were screened by cellular enzyme linked immunosorbent assay and indirect immunofluorescence on rat living cells.
      
Both monoclonals belong to the IgG2A isotype and were screened with insulin-producing rat insulinoma cells by an indirect immunofluorescence test as well as by a cellular enzyme linked immunosorbent assay.
      
Maximal exocellular β-lactamase production occurred at 48 h growth and exceeded cellular enzyme levels 7-fold.
      
The effects of propiconazole on extra-cellular enzyme levels in Trametes versicolor have been investigated during wood colonization and degradation.
      
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  cell-enzyme
The total and direct reacting bilirubin concentrations depended on the red cell-enzyme phenotypes (ACP1, PGM1, and GLO1) in both TC and control groups.
      
Methodologically, the visualization of PTL adhesion gave more reliable results for measurement of PTL adhesion than the cell-enzyme immunoassay (EIA) for P-selectin.
      
AECA titer was determined by cell-enzyme-linked immunosorbent assay and expressed as mean percentage of control from triplicate determinations.
      
  cell enzyme
Changes in their spectral properties are used for monitoring of cell enzyme activities or changes in concentrations of particular molecules.
      
Comparison of its properties towards substrates and inhibitors with those of brain Ca2+/calmodulin-dependent protein kinase II suggests that the beta-cell enzyme may be similar or identical to the brain enzyme.
      
One must assume that the mechanism of cell enzyme release into the blood is identical in all species.
      
Multiple sampling in single-cell enzyme assays using capillary electrophoresis with laser-induced fluorescence detection
      
The methods we used here, a cell enzyme-linked immunosorbent assay and flow cytometry, are simple methods for development and purification of a bimAb when a functional selection method is not a priori available.
      
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E. coli cells having high penicillin acylase activity were entrapped in gelatin gel and crosslinked with glutaraldehyde.The immobilized cells were used for the continuous hydrolysis of benzylpenicillin, no loss of enzymatic activity was found after 103 days at 37℃. They have been satisfactorily applied to industrial production of 6-aminopenicillanic acid, no significant reduction of the hydrolytic rate was observed after 285 cycles within seven and a half months.The enzymatic properties of immobilized E. coli...

E. coli cells having high penicillin acylase activity were entrapped in gelatin gel and crosslinked with glutaraldehyde.The immobilized cells were used for the continuous hydrolysis of benzylpenicillin, no loss of enzymatic activity was found after 103 days at 37℃. They have been satisfactorily applied to industrial production of 6-aminopenicillanic acid, no significant reduction of the hydrolytic rate was observed after 285 cycles within seven and a half months.The enzymatic properties of immobilized E. coli cells have been investigated and compared with those of intact cells. It was found that penicillin acylase activity of the immobilized cells was more stable than that of the intact cells.

高产青霉素酰化酶的大肠杆菌细胞用明胶包(?),并由戊二醛将其固定。这种固定化细胞用来连续水解苄基青霉素,在37℃使用103天没有发现损失酶活性。它们已被用于6-氨基青霉烷酸的工业生产,效果令人满意。在七个半月内使用285次,水解速率没有明显下降。研究了固定化大肠杆菌细胞的酶性质,并与相应的天然细胞作了比较。发现固定化细胞的青霉素酰化酶比天然细胞的更稳定。

Three species of nitrogen-fixing blue-green algae, Anabaena azotica (HB 686), Anabaenacylindrica and Anabaena 7120, were studied with reference to nitrogenase activity in intactcells as well as in cell-free extracts. Although the nitrogenase activity of intact cells of HB 686(51.9mmC_2H_4/O. D./30min.) was higher than A. 7120 (16.4mmC_2H_4/O. D./30min.), itsactivity in the crude extract was the lowest. This may be due to the abundance of mucilagesin the algal mass. The effects of Mn~(++) and Fe~(++) on the nitrogenase...

Three species of nitrogen-fixing blue-green algae, Anabaena azotica (HB 686), Anabaenacylindrica and Anabaena 7120, were studied with reference to nitrogenase activity in intactcells as well as in cell-free extracts. Although the nitrogenase activity of intact cells of HB 686(51.9mmC_2H_4/O. D./30min.) was higher than A. 7120 (16.4mmC_2H_4/O. D./30min.), itsactivity in the crude extract was the lowest. This may be due to the abundance of mucilagesin the algal mass. The effects of Mn~(++) and Fe~(++) on the nitrogenase activity of these algae weretudied also, and the reaction kinetics with different enzyme concentrations were estimated. Theresults obtained point out that in A. cylindrica there is a lack of activating cofactor, which hasbeen demonstrated in Rhodospirillum rubrum. Besides, the filaments of Anabaena 7120 weretreated with toluol-ethanol and their N_2-fixing activity in situ was estimated in an attempt tofind out the mechanism of protection from oxygen.

对三种固氮蓝藻:固氮鱼腥藻(水生686)、柱孢鱼腥藻和鱼腥藻7120的整细胞及无细胞抽提液的固氮酶活性,进行了比较研究。水生686的整细胞酶活虽然不低(51.9毫米乙烯峰高/光密度/30分),仅次于柱孢鱼腥藻,但其无细胞抽提液的酶活却最低。这可能与它含有大量藻胶有关。研究了Mn~(++)、Fe~(++)对蓝藻固氮酶的作用,以及测定其在不同酶浓度下的反应动力学表明:柱孢鱼腥藻中不存在象深红螺菌中所看到的那种激活因子。用甲苯-乙醇溶液处理藻细胞,对固氮酶作原位测定,探索了它的氧损伤及氧保护机理。

E.coli cells having high penicillin acylase activity were entrapped in gelatin geland crosslinked with glutaraldehyde.The immobilized cells were used for the continuous hydrolysis of benzylpenicillin,no loss of enzymatic activity was found after 103 days at 37℃. They have been satis-factorily applied to industrial production of 6-aminopenicillanic acid, no significantreduction of the hydrolytic rate was observed after 285 cycles within seven and a halfmonths.The enzymatic properties of immobilized E.coli cells...

E.coli cells having high penicillin acylase activity were entrapped in gelatin geland crosslinked with glutaraldehyde.The immobilized cells were used for the continuous hydrolysis of benzylpenicillin,no loss of enzymatic activity was found after 103 days at 37℃. They have been satis-factorily applied to industrial production of 6-aminopenicillanic acid, no significantreduction of the hydrolytic rate was observed after 285 cycles within seven and a halfmonths.The enzymatic properties of immobilized E.coli cells have been investigated andcompared with those of intact cells. It was found that penicillin acylase activity of theimmobilized cells was more stable than that of the intact cells.

高产青霉素酰化酶的大肠杆菌细胞用明胶包埋,并由戊二醛将其固定。这种固定化细胞用来连续水解苄基青霉素,在37℃使用103天没有发现损失酶活性。它们已被用于6-氨基青霉烷酸的工业生产,效果令人满意。在七个半月内使用285次,水解速率没有明显下降。研究了固定化大肠杆菌细胞的酶性质,并与相应的天然细胞作了比较。发现固定化细胞的青霉素酰化酶比天然细胞的更稳定。

 
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