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碘标记
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  “125碘标记”译为未确定词的双语例句
     Methods: Serum level of Epo in 114 hemopathic patients and 30 normal controls was determined by 125 I-RIA.
     方法:用放射免疫125碘标记法检测114例血液病性贫血患者血清中Epo水平,并将同期正常人30例为对照进行统计学分析。
短句来源
     Using the Radio Isotope125I to examinate 114 cases with anemia and normal adult EPO in anemia were higher than in normal abults (P<0. 01 and P<0.05).
     用放射免疫125碘标记法对114例血液病性贫血患者血清EPO进行测定,并与间期30例正常人对照。
短句来源
     Biodistribution of 125 I labeled 17α vinyestradiol 3 acetate ( 125 I VE 2A)in nude mice bearing human breast cancer containing different estrogen receptor (ER) content was studied to understand the relation between this compound and ER and, consequently, to develop the ER imaging.
     为探讨测定乳腺癌细胞内雌激素受体( E R),以判断肿瘤对内分泌治疗的敏感性和预后,利用1 25 碘标记 17α乙烯雌二醇3醋酸酯(1 2 5 I V E2 A)在不同 E R 含量的荷人乳腺癌裸鼠体内进行生物学分布研究,观察其与受体含量的关系,为进一步进行肿瘤 E R 显像奠定基础。
短句来源
     125I-radiolabeled amoxycillin is used to study for the penicillin-binding proteins (PBPs) of clinical isolated MRSA. The resistsnt strains did not produce detectable β-lactamase activity.
     目的:本文以放射性125碘标记阿莫西林研究-临床分离MRSA青霉素结合蛋白(PBPs),探讨高耐药及低耐药不产酶MRSA青霉素结合蛋白的改变。
短句来源
     Objective To detect the role of implantable biodegradable polymers for the local delivery of 125 I radiolabeled idoxuridine (IUdR).
     目的研究脑局部缓释125碘标记的5-碘脱氧尿苷(IUdR)的作用。
短句来源
  相似匹配句对
     ~(125)I-labelled cholylglycine
     -125标记甘胆酸
短句来源
     IODINATION OF DNA IN VITRO
     DNA的-125体外标记
短句来源
     Under the conditions as described, HCGwas radioiodinated to 2.35-15.1 μCi/μgwhich corresponded to 0. 054-0. 35 atoms of~(125)I per molecule assuming a mol wt of 40000daltons.
     对~(125)标记条件作了讨论。
短句来源
     Synthesis of 125I labelled derivatives of thyroxine and triiodothyronine for RIA
     -125标记甲状腺激素衍生物的合成
短句来源
     DISTRIBUTION OF ~(125)I-LABELLED VENOM PROTEINS OF PIT VIPER (Agkistrodon halys Pallas) IN MICE
     -125标记蝮蛇毒蛋白在小鼠体内的分布
短句来源
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  125 i-labelled
Isolated muscle cells from adult rat heart have been used to study the effect of temperature and enzymic digestion on the binding of125I-labelled insulin.
      
Purification of125I-labelled aprotinin by hydrophobic interaction chromatography
      
125I-labelled aprotinin as reagent for simultaneous determination of serine proteinases by hydrophobic interaction chromatograph
      
The suitability of125I-labelled aprotinin has therefore been tested as a reagent in the analysis of mixtures containing trypsin, α-chymotrypsin and kallikrein taken as models, in the presence of ribonuclease and lysozyme.
      
The intratumoral remnants of125I-labelled OK-NK cells were 61, 27 and 8% at 4, 12 and 36h after intratumoral transfer, respectively.
      
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A double antibody radioimmunoassay for detecting luteinizing hormone inrat serum was developed by using rabbit anti-ovine LH serum and radioiodinatedovine LH.In this heterogeneous system the dose-response curve of ovine LHwas parallel to that of rat LH.When ovine LH_(2-2-1)was used as a referencestandard,the sensitivity of the assay was found to be 0.1 ng per tube.The im-munoassay potency of LH_(2-2-1)was equal to that of NIH-LH-S_(18).By using ratLH-RP-1 as a reference standard,the sensitivity was found to...

A double antibody radioimmunoassay for detecting luteinizing hormone inrat serum was developed by using rabbit anti-ovine LH serum and radioiodinatedovine LH.In this heterogeneous system the dose-response curve of ovine LHwas parallel to that of rat LH.When ovine LH_(2-2-1)was used as a referencestandard,the sensitivity of the assay was found to be 0.1 ng per tube.The im-munoassay potency of LH_(2-2-1)was equal to that of NIH-LH-S_(18).By using ratLH-RP-1 as a reference standard,the sensitivity was found to be 10 ng per tube.Since rat FSH did not show crossreaction with anti-ovirie LH serum,the inter-ference of FSH appeared to be negligible in the measurement of serum LH.By using this assay system we have studied the changes of serum LH levels inrats after ovariectomy and with treatment of progesterone and diethylstilboestroltwo months after the operation.The results confirm that sex steroid hormonespossess a negative feedback mechanism to pituitary LH secretion.The effect ofgossypol on LH secretion has also been investigated.It has been found that inadult male rats there are no significant changes either on the serum LH level or inthe response of pituitary to LRH injection following gossypol administration.The data seem to indicate that this new antifertile agent for male,gossypol,does not influence the LH secretion of pituitary.

用兔抗羊 LH 血清和放射性碘标记的羊 LH 建立了大白鼠血清中 LH 的双抗体放射免疫测定法。在这异源的系统中羊 LH 和大鼠 LH 的剂量反应曲线是平行的。当用羊LH_(2-2-1)作参考标准时测量的灵敏度为0.1ng/管。当用大鼠 LH-RP-1作参考标准时测量的灵敏度为10ng/管。大鼠的 FSH 没有交叉反应,所以 FSH 不会干扰血清 LH 的测定。用这个系统我们研究了去卵巢以及去卵巢后给予孕酮和巳烯雌酚以后大鼠血清中LH 水平的变化。结果表明性类固醇激素对大鼠垂体 LH 的分泌具有一个负反馈的调节机制。本文中还研究了棉酚对大鼠 LH 分泌的影响。我们发现成年雄鼠服用棉酚以后血浆 LH 的水平以及垂体对 LRH 的反应均无显著的变化。结果表明新的男性抗生育药物——棉酚似乎不影响垂体的 LH 分泌功能。

It was reported that cobalt chelate of ~(131)I labeled succinyl bleomycin A_5 (Co-~(131)I-BL (Ⅱ) had high affinity for cancer cells. In order to identify the effect of cobalt on the behavior of bleomycin, a comparison between the compounds labeled before and after cobalt chelation was made. It was found that the radioactive iodine could successfully label the bleomycin before chelation. On the contrary the labeling rate decreased to less then 1% if the bleomycin was previously chelated. In vivo, the uptake of...

It was reported that cobalt chelate of ~(131)I labeled succinyl bleomycin A_5 (Co-~(131)I-BL (Ⅱ) had high affinity for cancer cells. In order to identify the effect of cobalt on the behavior of bleomycin, a comparison between the compounds labeled before and after cobalt chelation was made. It was found that the radioactive iodine could successfully label the bleomycin before chelation. On the contrary the labeling rate decreased to less then 1% if the bleomycin was previously chelated. In vivo, the uptake of Co-~(131)I-BL(Ⅱ) in tumor-bearing mice was compared to that of cobalt free ~(131)I-BL(Ⅱ) and it was proved that the cobalt chelation could remarkably enhance the affinity of bleomycin for cancer cells. The clearance rate of Co-~(131)I-BL (Ⅱ) in vivo was very fast as most of the radioactivity was excreted from the kidneys 30 minutes after injection. Two hours after injecticn, the remaining radioactivity in normal tissue and blood decreased to 1% or even less. The cobalt chelate of ~(131)I-BL(Ⅱ) had been shown to be stable in vitro, as no detectable degradation could be observed in paper chromatography autoradiogram after incubation with normal serum at 37℃ for 2 hours or standing at 4℃ for 10 days. Our data demonstrated that cobalt chelation was essential for augmentating markedly the oncophilic properties of bleomycin. The previous concept that bleomycin itself is a good oncophilic agent should be re-appraised.

本文报道~(131)碘标记争光霉素沪Ⅱ钴螯合物的亲肿瘤性与~(131)碘标记和钻螯合次序的先后有关.实验证明,只有先标记~(131)碘再螯合钴才能使标记获得成功,且~(131)碘标记争光霉素一旦与钴螯合后,其亲肿瘤作用显著增强.同时,还对螫合物的稳定性、体内分布和血液廓清情况进行了讨论.

The isolation purification, iodination and identification of a-Bungarotoxin(a-BTX)were recorded.Purified a-BTX was prepared from the venom of Hunan Bu-ngarus multicinctus (Blyth) by CM-Sephadex C~50 ionexchange chromatography and Sephadex G~25 chromatography.The homogeneous a-BTX was obtained by rechromatography of purified ct-BTX (fraction 3) on a CM-Cellulose CM-32, and was labelled with 1251 by chloramine-T method.The 1251-α-BTX was then separated from Na125I and purified by Sephadex G-25 column.The results...

The isolation purification, iodination and identification of a-Bungarotoxin(a-BTX)were recorded.Purified a-BTX was prepared from the venom of Hunan Bu-ngarus multicinctus (Blyth) by CM-Sephadex C~50 ionexchange chromatography and Sephadex G~25 chromatography.The homogeneous a-BTX was obtained by rechromatography of purified ct-BTX (fraction 3) on a CM-Cellulose CM-32, and was labelled with 1251 by chloramine-T method.The 1251-α-BTX was then separated from Na125I and purified by Sephadex G-25 column.The results yield a specific activity of 90~100 Ci/mM.40~50% labelling.40~60% recovery of protein and less than 3 % contamination with free iodine in 125I-α-BTX.The purified a-BTX was identified by determining its N-terminal amino acid, amino acid composition, isoelectric and polyacrylamide gel electrophoresis.Biological activity of the α-BTX was determined by its toxicity to mice, its neuromuscular blocking action and binding to acetylcholine receptors at the motor entplate of the rat diaphragm.

本文介绍了α-银环蛇毒素的分离提纯、碘标记和鉴定。用羧甲基葡聚糖C-50离子交换层析和葡聚糖G-25层析,从湖南银环蛇毒液中制备出纯α-银环蛇毒素。均质α-银环蛇毒素是用羧甲基纤维素CM-32重层析纯α-银环蛇毒素(第3峰)而获得。 用氯胶-T法进行125~I标记α-银环蛇毒素。经Sephadex G-25柱层析分离纯化,产品放射性比度为90~100Ci/mM,标记率40~50%,蛋白回收率40~60%,游离碘含量小于3%。用测定N-末端氨基酸,氨基酸成分,等电点和聚丙烯酰胺凝胶电泳进行纯α-银环蛇毒素的鉴定。以小白鼠毒性,神经肌肉阻断作用和结合胆硷能受体等测定α-银环蛇毒素的生物活性。

 
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