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   125碘标记 在 基础医学 分类中 的翻译结果: 查询用时:0.105秒
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碘标记
相关语句
  i-labelled
    ~(125)I-LABELLED MONOCLONAL ANTIBODY IN THE DETECTION OF LEPTOSPIRAL ANTIGENS IN TISSUES OF GUINEA PIG
    ~(125)碘标记单克隆抗体检测钩端螺旋体抗原在豚鼠体内的分布
短句来源
  “125碘标记”译为未确定词的双语例句
    STUDY ON THE STABILITY OF RADIOIODINE-LABELED PORCINE ZONA PELLUCIDA ANTIGEN
    碘标记猪卵透明带抗原的稳定性研究
短句来源
    Effects of purified humanapoliporoteins AⅠ, CⅠ, CⅡ, CⅢ_(-1),, Csand E on the binding of ~(125)Ⅰ. -labeled apoE-deficient HDL, to isolated rat liverplasma membranes were investigated.
    以~(125)碘标记人不含apoE HDL_3为配体,研究纯化人apoAI、CⅠ、CⅡ、CⅢ、Cs及E对大鼠肝细胞膜HDL受体结合功能的影响。
短句来源
    In order to investigate the relationship between scavenger receptor type A and cell signal transduction, human U937 macrophages were treated with tyrosine protein kinase inhibitor genistein, then the cells were incubated with [ 125 I]ox LDL or ox LDL, and the cellular degradation of [ 125 I]ox LDL or binding were measured separately.
    为研究清道夫受体与细胞内酪氨酸蛋白激酶的关系 ,用酪氨酸蛋白激酶抑制剂genistein处理人U937细胞。 分别测定对照组和处理组细胞对碘标记的氧化低密度脂蛋白 [12 5I]ox LDL的结合、降解以及细胞内脂质蓄积的程度 ;
短句来源
    The metabolism of the antibodies in SKOV_3 was assayed by cellular RIA.
    采用氯胺T法进行碘标记抗体,用细胞放射免疫分析实验,检测抗体在SKOV3细胞中的代谢。
短句来源
    DETECTION OF EBV-DNA IN EPITHELIAL CELLS FROM NASOPHARYGEAL CARCINOMA BY IN SITU HYBRIDIZATION
    用碘标记核酸检查鼻咽癌上皮细胞中的EB病毒基因
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  i-labelled
Isolated muscle cells from adult rat heart have been used to study the effect of temperature and enzymic digestion on the binding of125I-labelled insulin.
      
Purification of125I-labelled aprotinin by hydrophobic interaction chromatography
      
125I-labelled aprotinin as reagent for simultaneous determination of serine proteinases by hydrophobic interaction chromatograph
      
The suitability of125I-labelled aprotinin has therefore been tested as a reagent in the analysis of mixtures containing trypsin, α-chymotrypsin and kallikrein taken as models, in the presence of ribonuclease and lysozyme.
      
Imaging studies to evaluate for EAPs include CT, MRI, and 131I-labelled metaiodobenzylguanidine scintigraphy.
      
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  i labelled
UEA I labelled endomysial capillaries and endothelial cells of perimysial blood vessels in all the examined muscles.
      
The heparin binding capacity of PEU/PIME/al was evaluated with 125I labelled heparin, using for comparison the analogous polymer obtained with a diamide-diol (i.e.
      
Using125I labelled monospecific antisera against human,μ-,γ-,α-,κ- andλ-chains we investigated the percntage of surface Ig-bearing lymphocytes in patients suffering from primary or acquired hypogammaglobulinaemias.
      
Autoradiography after injection of 125I labelled copper-zinc superoxide dismutase into normal rats showed no evidence that the enzyme enters viable islet cells, suggesting an extracellular site of protection against alloxan.
      
Scintigraphic distribution of 123 I labelled proinsulin, split conversion intermediates and insulin in rats
      
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Ten biopsies from the patients with nasopharygeal carcinoma were examined by nucleic acid hybridization. In situ hybridization was used for the localization of EBV genome in the epithelial cells of NPC.The epithelial cells from 9 biopsies harbour EB viral genome demonstrated by in situ hybridiztion.The DNA e :tracted from 4 biopsies was spotted on nitrocellulose paper and hybridized with EBV-DNA fragment.The nucleic acid hybridization were developed by using phage ss-DNA containing inserted fragment of EBV-DNA...

Ten biopsies from the patients with nasopharygeal carcinoma were examined by nucleic acid hybridization. In situ hybridization was used for the localization of EBV genome in the epithelial cells of NPC.The epithelial cells from 9 biopsies harbour EB viral genome demonstrated by in situ hybridiztion.The DNA e :tracted from 4 biopsies was spotted on nitrocellulose paper and hybridized with EBV-DNA fragment.The nucleic acid hybridization were developed by using phage ss-DNA containing inserted fragment of EBV-DNA and labeled with 12SI as probe.The labeled DNA was used for in situ hybridization and spot hybridization on filter.As a result, a more sensitive and effective probe (over 1×108 cpm/μg DNA ) was obtained.

用同位素碘化钠直接标记核酸。以细胞内原位杂交实验检查鼻咽癌上皮细胞中的EB病毒基因。共检查病理确诊的10例鼻咽癌活检标本的细胞涂片,其中9例直接证实有EB病毒核酸。本文建立并改进了细胞内原位核酸杂交技术,首先使用重组的单链DNA作探针,并将碘标记的核酸用于原位核酸杂交实验。

Spleen cells of BALB/c mice immunizedwith whole leptospira interrogans serovarLai strain 017 were fused with myelo-ma cell lines SP2/0. Hybridoma (L-B_1)secreted monoclonal antibody which wascharacterized as IgG_2b. The antibody reactedspecifically with leptospiral antigens inELISA (1: 64×10~4) and was purified withascitic fluid and injected intravenously intoguinea pigs with the PDH before the oozingof blood from the nostrils and mouth. Radio-activity of differed tissues was measuredwith FH408 medical well-typed-γ-counter....

Spleen cells of BALB/c mice immunizedwith whole leptospira interrogans serovarLai strain 017 were fused with myelo-ma cell lines SP2/0. Hybridoma (L-B_1)secreted monoclonal antibody which wascharacterized as IgG_2b. The antibody reactedspecifically with leptospiral antigens inELISA (1: 64×10~4) and was purified withascitic fluid and injected intravenously intoguinea pigs with the PDH before the oozingof blood from the nostrils and mouth. Radio-activity of differed tissues was measuredwith FH408 medical well-typed-γ-counter. The results were 112. 5±19. 5×10~3 cpm/200mg (Liver); 110±36. 7×10~3 cpm/200mg(Kidney); 82. 2±16×10~3 cpm/200mg(Lung);79.6±55. 4×10~3 cpm/200mg (Intestine) and52. 5±14×10~3 cpm/200mg (Spleen). Experi-ments in guinea pigs have demonstratedthat McAb provides a degree of specificityand sensitivity greater than that of conven-tionally prepared antibodies.

作者用~(125)碘标记抗黄疸出血群赖型017株钩体的McAb(LB_1株IgG_2b)静脉注入钩体病肺弥漫性出血型豚鼠,证实钩体抗原主要分布在肝、肾、肺及脾器官内。以往抗血清抗体放射自显影实验在肺内钩体抗原很少,本结果表明肺内有不少钩体抗原(平均为82.2+16×10~3cpm/200mg),揭示了用单克隆抗体结合~(125)碘探讨钩体特异性抗原在肺组织分布,具有高特异性和敏感性。

Purified porcine zona pellucida antigen(PPZA)was labeled with radioiodine by ch-loramine-T,lactoperoxidase and iodine monochloride methods.The immunoactivity andstability of the ~(125)I-PPZA labeled by the three methods were compared.Results showedthat lactoperoxidase method was the best in respect of stability and immunoactivity of~(125)I-PPZA,which should be considered suitable to be used for radioimmunoassay.

本工作研究了纯化的猪卵透明带抗原(PPZA)的放射性碘标记方法,比较了氯胺T法、一氯化碘法和乳过氧化物酶法制备的~(125)I-PPZA 的免疫活性及稳定性,发现用乳过氧化物酶法制备的~(125)I-PPZA 在稳定性和比放射性方面均优于其他两种方法,可满足猪卵透明带抗原的放射免疫测定的要求。

 
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