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烟草再生植株
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  “烟草再生植株”译为未确定词的双语例句
     A lot of para-nodules of approximate hemosphere and sphere could form on their roots of regeneration plantlets of tobacco in Jensen medium contained 0.3mg/L 2,4-D and 5×10 8/L R. Leguminosarum.
     烟草再生植株在含有 0 .3mg/L 2 ,4 D和 5× 10 8个 /L豌豆根瘤菌的Jensen培养基中 ,可以在其根部形成大量近似半球形和球形的类根瘤 .
短句来源
     The effect of nodulation was relation with the consistency of 2,4-D and rhizobium, the species of rhizobium and the time added rhizobium and 2,4-D.
     烟草再生植株的结瘤除受制于 2 ,4 D和根瘤菌的浓度外 ,还与根瘤菌的种类和加入根瘤菌与 2 ,4 D的时期有关 .
短句来源
     PCR amplification results with primer designed according to the fad2 gene fragment and NPTⅡ gene in pBI121 demonstrated that antisense fad2 was integrated into tobacco genomes.
     将所得片段经BamHI和SacI酶切后插入表达载体质粒pBI121,构建反义表达载体pBI121fad2,并利用农杆菌介导法转入烟草叶片,获得了抗卡那霉素和安苄青霉素的烟草再生植株
短句来源
     Here, we inserted the RRD3 fragment into the plant promoter-capture vector, pCAMBIA1391Z, and examined whether the RRD3 fragment has promoter activity in plants. Transgenic tobacco and rice calli both showed β-glucuronidase (GUS) activity, indicating that RRD3 can act as a promoter in both monocot and dicot plants.
     我们将RRD3片段插入到植物启动子捕获载体pCAMBIA1391Z中,检测RRD3片段是否在植物中也有启动子活性,转基因烟草再生植株和水稻愈伤组织均显示了gusA基因的表达,表明其在单子叶和双子叶植物中均可行使启动子功能。
短句来源
     transformed regeneration plants derived from three varieties of tobacoo whic h were transformed by the leaf-method and Kanamycin selection were tested throug h PCR electrophoresis assay, under the media of plasmoid PBLGC (PBLGC contained chitinase gene and β-1, 3-glucanase gene and NTP-II gene) by using promtor 35S, chitinase gene and β-1, 3-glucanase gene primer separately. 92, 72 and 47 posi tive plants were obtained respectively.
     对采用叶盘法以质粒PBLGC为介导转化烟草的 3个品种 ,经Kan抗性筛选获得的 119株转化的烟草再生植株分别以启动子CaMV3 5S、几丁质酶基因和β -1,3 -葡聚糖酶基因的引物进行PCR检测 ,分别获得 91、72、47株阳性植株。
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  相似匹配句对
     Regenerated Plant from Protoplast of Nicotiana alata
     花烟草原生质体的再生植株
短句来源
     Plant regeneration of Nicotiana tabacum through somatic embryogenesis
     烟草体细胞胚胎发生再生植株
短句来源
     : Plant Regeneration from Mesophyll protoplasts in N. glauca.
     粉兰烟草原生质体再生植株
短句来源
     Hairy root induction and plant regeneration in Nicotiana tabacum
     烟草发状根的诱导和植株再生
短句来源
     The Factors Affecting Plant Regeneration from Somatic Hybridization in Tobacco
     烟草体细胞杂交再生植株的影响因素
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The Soybean Kunitz Trypsin Inhibitor gene was obtained from soybean immature cotyledons total RNA by RT-PCR, and the amplified DNA fragment was cloned into Smal site of pBluescript KS (+). DNA sequence analysis indicates that the cloned DNA fragment composed of 663hp has 99. 5% homologies in nucleotide sequence and 98. 2% in amino acid sequence respectively compared with previously published sequence. A plant expression vector pBinSKTI was constrncted and used in plant transformation. The transgenic tobacco...

The Soybean Kunitz Trypsin Inhibitor gene was obtained from soybean immature cotyledons total RNA by RT-PCR, and the amplified DNA fragment was cloned into Smal site of pBluescript KS (+). DNA sequence analysis indicates that the cloned DNA fragment composed of 663hp has 99. 5% homologies in nucleotide sequence and 98. 2% in amino acid sequence respectively compared with previously published sequence. A plant expression vector pBinSKTI was constrncted and used in plant transformation. The transgenic tobacco was confirmed by PCR and scutnern blotting. Results of Bioassays show that the transgenic tobacco displays notable insectrestnnt ability to the Larvae of Heliothis armigera compared with wild tobacco.

从未成熟的大豆子叶中提取总RNA,利用RT-PCR方法扩增出大豆Kunitz型胰蛋白酶抑制剂基因单一目的片段,克隆到pBluescriptKS(+)SmaI位点上。序列分析表明,该基因与报导的序列高度同源:其核苷酸序列及推论的编码氨基酸序列的同源率分别为99.5%和98.2%。由此,构建了大豆Kunitz型胰蛋白酶抑制剂基因的植物高效表达载体pBinSKTI。并采用农杆菌介导的叶盘法转化了烟草,获得的转基因烟草再生植株经抗虫测试表明:与对照烟草相比,具有明显的抗棉铃虫能力。

he production of enzyme capable of degrading the cell walls of invading phytopathogenic fungi is an important component of the defense response of plants.The baculovirus derived chitinase,which can dissolve fungal wall,was amplified by polymerase chain reaction(PCR),inserted into plasmid vector pROK2,and transferred into Escherichia coli XL1 Blue.The recombinant plasmid pROK2 DNA were mobilized into Agrobacteria tumefaciens LBA 4404 by using Freeze thaw for transformation method.With the help of infection...

he production of enzyme capable of degrading the cell walls of invading phytopathogenic fungi is an important component of the defense response of plants.The baculovirus derived chitinase,which can dissolve fungal wall,was amplified by polymerase chain reaction(PCR),inserted into plasmid vector pROK2,and transferred into Escherichia coli XL1 Blue.The recombinant plasmid pROK2 DNA were mobilized into Agrobacteria tumefaciens LBA 4404 by using Freeze thaw for transformation method.With the help of infection of A.tumefaciens  the chitinase were transferred into tobacco leaf disks.By tissue culture,shoots were induced.The induced shoots were regenerated roots in MS Medium containing kanamicin sulfate(50μg/ml)The results of PCR and western blot assays showed the chitinase gene was expressed in flue cured tobacco varieties CF 80,K326 and Xanthinc.The chitinse enzyme activity in transformed tobacco plants is higher than untransformed ones.

利用多聚酶链式反应(PCR)对来源于一种生物杀虫剂(baculovirus)的几丁质酶基因进行体外扩增,并插入载体质粒pROK2中,然后转化大肠杆菌(Escherichiacoli)XL1Blue。用重组质粒pROK2DNA转化植物转基因载体——土壤农杆菌(Agrobacteriatumefaciens)LBA4404菌株。借助于土壤农杆菌侵染烟草叶圆片,将目的基因导入。通过组织培养诱导生芽和生根,获得烟草再生植株。利用含卡那霉素的培养基对再生烟株进行初步筛选。进一步PCR和Western印迹检测结果表明:转基因烟草中有几丁质酶基因和蛋白的表达。初步检测结果表明:转基因烟草具有较高的几丁质酶活性。

A lot of para-nodules of approximate hemosphere and sphere could form on their roots of regeneration plantlets of tobacco in Jensen medium contained 0.3mg/L 2,4-D and 5×10 8/L R.Leguminosarum. The effect of nodulation was relation with the consistency of 2,4-D and rhizobium, the species of rhizobium and the time added rhizobium and 2,4-D.

烟草再生植株在含有 0 .3mg/L 2 ,4 D和 5× 10 8个 /L豌豆根瘤菌的Jensen培养基中 ,可以在其根部形成大量近似半球形和球形的类根瘤 .烟草再生植株的结瘤除受制于 2 ,4 D和根瘤菌的浓度外 ,还与根瘤菌的种类和加入根瘤菌与 2 ,4 D的时期有关 .

 
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