Methods Forty male SD rats aged 12-month were randomly divided into four groups by MDA contents, which were low dose, middle dose and high dose treatment groups and a control group, after continously intragastric infusion for 60 days, serum MDA, SOD and GSH-Px were determined.
The duration of administration was 4 weeks, and the weight of mice was measured weekly. After the last administration of the medicines, the serum levels of ALT and AST were measured, the liver index was calculated, and the GSH-PX, GST, and MDA of the liver were measured.
The level of blood glucose,the total antioxidative capacity(T-AOC),the activity of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),and xanthine oxidase(XOD),and the content of malondiadehyde(MDA) were detected respectively in liver and testis.
RESULTS:BA could reverse the GM induced low activities of T SOD (from 46.50 to 27.86 NU/mL,P< 0.01) and GSH PX(from 83.77 to 42.34 U,P< 0.01) ,and limited the GM induced high content of MDA(from 11.87 to 17.03 μmol/L,P< 0.01) in SGC.
The activities of blood GSH Px in quenching,welding and sealing groups were (71.42±26.52)NU/ml,(70.77±20.12)NU/ml and (71.67 ±18.87)NU/ml respectively,significantly lower than that in the control[(97.02±21.76)NU/ml, P <0.05 or P <0.01].
Method: 23month old SD rats are observed as naturally senile rate, of brainmitochondria membrane are conjugateddienes (CD), molondialdehyde (MDA), glutathione peroxidase (GSH Px), superoxide dismutase (SOD), Na + K + ATP ase and Ca 2+ Mg 2+ ATP ase measured as indexes to observe the effect of the PNPY.
The rats were killed 48 hours after the last injection,and the activity of glutamic-pyruvic transaminase(GPT) in serum and the contents of mercury,malondialdehyde(MDA),and glutathione(GSH) and the activities of glutathione-peroxidase(GSH-Px) and superoxide dismutase(SOD) in the liver and renal cortex were determined.
Lipid peroxide (LPO), superoxide dismutase (SOD) and glutathione-peroxidase (GSH-PX) activities were determined in renal cell carcinoma (RCC) and tumor-uninvolved renal tissues in 21 patients with renalcancer. The results showed that LPO in RCC was increased than in nomal renal tissues (P<0.05).
Hepatic glutathione, lipid peroxides, glutathione peroxidase, alcohol dehydrogenase, aldehyde dehydrogenase, glycogen and total protein in liver were also significantly altered.
The activity of glutathione-dependent enzymes (glutathione peroxidase, glutathione reductase, and glutathione transferase) did not differ in the brain of SAMP1 and SAMR1 animals, and catalase activity was similarly low in both cases.
Low SOD and catalase activity and high activity of glutathione-dependent enzymes in tumors suggest that glutathione peroxidase and glutathione S-transferase play a major role in peroxide utilization in malignant tumors.
Significant increase in mRNA level and activity of Mn-superoxide dismutase (Mn-SOD), catalase, and selenium-dependent glutathione peroxidase-1 (GPx-1) and reduced ROS level was found in resistant K562/DOX and SKVLB cells.
This review considers the peculiarity of functions of mitochondrial GSH and enzymes of its metabolism, especially glutathione peroxidase 4, glutaredoxin 2, and κ-glutathione transferase.
In this stage of leucosis, the activities of Cu,Zn-SOD, GSH-Px, and G-6-PDH in cytosol were unchanged; at the same time, the GR activity and the concentration of reduced glutathione smoothly decreased.
Co-treatment with melatonin inhibited the elevation of lipid peroxidation and significantly increased GSH-Px activity in the brain regions studied.
Hyperglycemia resulted in significant increases in the antioxidative enzymes, Cu, Zn-SOD, CAT, GSH-Px, and GSSG-R after four and eight weeks, respectively.
For all the subjects, the superoxide dismutase (SOD-1), glutathione peroxidase (GSH-Px), catalase (CAT) activities in the erythrocytes and the antioxidant activity of the blood plasma were determined.
During the carvedilol therapy, an increase in the SOD-1, GSH-Px and CAT activities was observed.
Enhancement of glutathione-peroxidase activity was induced by intrastriatal kainate injection, not only in the cortical area of control and melatonin-treated rats, but also in striatum of control rats.
We have tested normal and FA cells for oxygen consumption and for the activity of the antioxidant phospholipid-hydroperoxide-glutathione-peroxidase (PHGPx).
The various diets did not influence the activity of liver catalase (EC 18.104.22.168) nor superoxide dismutase (EC 22.214.171.124), but glutathione-peroxidase activity (EC 126.96.36.199) was higher in monkeys fed the MO diet.
Plasma catalase activity in both sexes was significantly higher (P>amp;lt;0.01) in RA and OA groups while RBC glutathione-peroxidase showed significantly (P>amp;lt;0.001) higher activity only in the female RA group, as compared to the control subjects.
A difference was found between RA and OA groups in malondialdehyde content and in glutathione-peroxidase activity in subjects of both sexes.