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肿瘤
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A comparative study of phosphate-activated glutaminase activity has been conducted in some normal and tumour tissues. It was found that:(1) There was a wide range of difference in the glutaminase activity among the tumours so far investigated, which was however not as wide as that observed in the normal tissues. The highest level of glutaminase activity was found in the transplanted thymolymphosarcoma, which was about seven times higher than that in the normal thymus.(2) During the growth of transplanted solid...

A comparative study of phosphate-activated glutaminase activity has been conducted in some normal and tumour tissues. It was found that:(1) There was a wide range of difference in the glutaminase activity among the tumours so far investigated, which was however not as wide as that observed in the normal tissues. The highest level of glutaminase activity was found in the transplanted thymolymphosarcoma, which was about seven times higher than that in the normal thymus.(2) During the growth of transplanted solid tumours, the glutaminase activity was found to vary with the weight of tumour tissue. An approximate parallelism between the increase in glutaminase activity and the weight of tumour has been observed in some, but not all of the tumours so far studied.(3) The enzyme activity in the regenerating rat liver was lower than that in the normal liver.(4) The possible significance of glutaminase to the growth of some tumours has been discussed.

(1) 本实验比较了一些正常组织与癌瘤组织中谷氨酰胺酶活性。结果发现移植性癌瘤的酶活性以淋巴肉瘤最高。肿瘤间酶活性的变动范围不如正常组织间的变动范围大。诱发性淋巴肉瘤的酶活性较其来源组织为高,但皮肤癌则较正常表皮低。(2) 在肿瘤生长过程中,谷氨酰胺酶活性随肿瘤的重量而有所变化,如艾氏腹水癌(实质型)、脑瘤22、胸腺淋巴肉瘤等的谷氨酰胺酶活性有随肿瘤生长而升高的趋势,但这并不是肿瘤的一般规律,如脑瘤53、Walker癌、肉瘤180等则无此现象。(3) 再生肝的谷氨酰胺酶活性较正常肝低。(4) 讨论了谷氨酰胺酶与某些肿瘤生长的可能关系。

According to the results of tissue culture,determining the index of anti-leukemiccells and method of spermatogonium,the total alkaloids of Tylophora ovata showedantitumour activity and inhibitory action against sarcoma 180,U14,L615 and Walkercarcinosarcoma 256.The main toxicity of total alkaloids was the emetic effect.Itinhibited also the phagocytic function of macrophages in vivo,but could be recoveredafter withdrawal of the drug.The antitumour activity,pharmacological actions andtoxic reactions of tylophorinidine...

According to the results of tissue culture,determining the index of anti-leukemiccells and method of spermatogonium,the total alkaloids of Tylophora ovata showedantitumour activity and inhibitory action against sarcoma 180,U14,L615 and Walkercarcinosarcoma 256.The main toxicity of total alkaloids was the emetic effect.Itinhibited also the phagocytic function of macrophages in vivo,but could be recoveredafter withdrawal of the drug.The antitumour activity,pharmacological actions andtoxic reactions of tylophorinidine and tylophorinine were similar to those of the totalalkaloids.

娃儿藤总碱在组织培养、抗白指数测定和精原细胞法中均显示有抗肿瘤活性,且对小鼠S180、U14、L615和大鼠W256都有抑制作用。总碱的主要毒性为呕吐反应。并对机体巨噬细胞的吞噬功能有抑制作用,但停药后可恢复。娃儿藤宁定和娃儿藤宁的抗肿瘤活性和药理、毒性作用与总碱类似。

0.05 ml of blood was taken by finger tip puncture and the whole blood wasincubated for 94 hours at 37℃.Sixteen hours before the cells were harvested 0.6μc~3H-thymidine was then added to each of the cultures.At the end of the culture period,radioactivity outside the cell in the culture was removed.Then trichloroacetic acid wasemployed to obtain DNA,the ~3H-thymidine radioactivity of which was determinedby liquid scintillation counting.The radioactivity represented the lymphocytetransformation.One hundred and...

0.05 ml of blood was taken by finger tip puncture and the whole blood wasincubated for 94 hours at 37℃.Sixteen hours before the cells were harvested 0.6μc~3H-thymidine was then added to each of the cultures.At the end of the culture period,radioactivity outside the cell in the culture was removed.Then trichloroacetic acid wasemployed to obtain DNA,the ~3H-thymidine radioactivity of which was determinedby liquid scintillation counting.The radioactivity represented the lymphocytetransformation.One hundred and twenty-five tests(118 persons)were carried out.The averagenormal value of lymphocyte transformation was determined.The lymphocytetransformation of some hemopoietic and tumor patients exhibited definite lowering.It was also found that ionizing radiation affected lymphocyte transformation.

本文介绍关于微量血液淋巴细胞转化的放射性测量法:指尖采血0.05毫升,全血培养37℃,94小时,收获细胞前16小时加入氚-胸腺嘧啶核苷(~3H-TdR)0.6微居里。培养结束时洗脱培养液中游离的放射性,然后用三氯醋酸提取细胞中的DNA,用液体闪烁计数器测定其中所含的~3H-TdR 的放射性,即可反映淋巴细胞转化。在125人次的测定中,初步得出输血者淋巴细胞转化的正常值,并发现某些血液病人及肿瘤病人的淋巴细胞转化能力明显降低,电离辐射对它亦有抑制作用。

 
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