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彗星检测
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  comet assay
     Comet assay or single-cell gel electrophoresis is now widely used to detect DNA damage in cultured animal cells, but only a few reports describe the application of comet assay in plant cells.
     单细胞凝胶电泳(彗星检测,comet assay)技术已广泛应用于动物细胞DNA损伤检测,但在植物细胞DNA损伤检测中的应用尚不多见。
短句来源
     COMET ASSAY ON THE DNA DAMAGE INDUCED BY UV RADIATION IN PLANT CELLS
     紫外辐射诱导植物细胞DNA损伤的彗星检测
短句来源
     The single cell gel electrophoresis or comet assay is now widely used to detect DNA damage in animal cells induced by radiation or chemicals.
     单细胞凝胶电泳(彗星检测)已广泛应用于动物细胞DNA损伤检测.
短句来源
     The specificity and sensitivity of comet assay on plant cell was improved by using protoplasts and T4 Endonuclease V.CASP software was used to analyze the comet image.
     以植物细胞原生质体为材料,并结合T4 Endonu lease V的运用,显著提高了UV-B诱导植物细胞DNA损伤提高彗星检测的敏感性和特异性.
短句来源
  “彗星检测”译为未确定词的双语例句
     Both UV-A and UV-B irradiation induced DNA damage in mesophylls.
     彗星检测结果表明,九里香叶片DNA的损伤程度与UV-B辐射的剂量呈正相关;
短句来源
     RESULTS: UV( 0.3 mW/cm2)induced a significant DNA damage (P<0.01) when the results were assayed by the four parameters (tail length,comet length,tail moment,olive tail moment). CONCLUSION: The results suggested that the four parameters indicated a good time-dependent effect. The method which we developed and the four parameters were reliable.
     结论0.3mW/cm2的紫外线照射K562细胞3s即可造成细胞DNA损伤,CASP分析软件可以用于彗星检测分析,其中尾长、彗星长、尾矩、Olive尾矩可作为彗星实验的分析指标,所改良的彗星实验系统可靠性较强,且基本上解决了实验中常见的脱胶现象,采图更方便。
短句来源
     Here, the accepted animal cell protocol was modified to adapt it to plant cells and the sensitivity to UV-B induced DNA damage was investigated in young and mature leaves of Murraya panicuata.
     本研究通过对动物细胞彗星检测方法的改进,利用植物细胞原生质体作为材料,研究了不同发育期九里香(Murraya panicuata)叶片对UV-B诱导的DNA损伤的敏感性差异。
短句来源
  相似匹配句对
     Detection of apoptotic cells by comet assay
     彗星试验检测细胞凋亡的研究
短句来源
     Detection of DNA crosslinks with comet assay
     彗星试验检测DNA交联的研究
短句来源
     spectroscopy detection.
     能谱检测
短句来源
     3)Surveillance and Monitoring;
     检测和监测 ;
短句来源
     The Statistics of Comet Orbits
     彗星轨道的统计
短句来源
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  comet assay
The single cell gel electrophoresis or comet assay was used to quantify DNA strand breaks as a measure of DNA damage induced by Cd and imidacloprid contamination in soil.
      
Roots ofVicia faba were exposed to the contaminated soil for 2 h at 25°C and were used in the comet assay.
      
By means of comet assay, a study of kinetics curve of DNA damage repair in irradiated SX-9 cells that came from mouse breast cancer proceeded.
      
Using the alkaline comet assay, we showed that antioxidants - vitamins C and E, quercetin, and melatonin - reduced the genotoxic effect of MNNG in H.
      
Carotenoid concentrations of lymphocytes were determined by HPLC and DNA damage was evaluated by the comet assay following an ex vivo treatment with H2O2.
      
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Objective:To investigate the variation of apoptotic comet in acute leukemia (AL) undergoing chemotherapy and the ralationship between apoptotic rate and treatment outcomes.Methods:Using improved singh′s method,the apoptotic comet rate was measured in 15 patients with AL after chemotherapy.Result:Before chemotherapy,the apoptotic comet rate were< 0.5 % in all cases. It started to increase at 8 hours and was up to peak at 24 hours after chemotherapy. In 15 patients,11 cases achieved CR after one course and all...

Objective:To investigate the variation of apoptotic comet in acute leukemia (AL) undergoing chemotherapy and the ralationship between apoptotic rate and treatment outcomes.Methods:Using improved singh′s method,the apoptotic comet rate was measured in 15 patients with AL after chemotherapy.Result:Before chemotherapy,the apoptotic comet rate were< 0.5 % in all cases. It started to increase at 8 hours and was up to peak at 24 hours after chemotherapy. In 15 patients,11 cases achieved CR after one course and all of their apoptotic comet rate> 98.0 %,MBDI> 65.0 %;Apoptotic comet rate 95.5 %、MBDI 18.5 % was observed in one case that showed CR after two courses;One case achieved PR after two courses and his apoptotic comet rate was 84.0 %,MBDI was 6.1 %;Two cases were unremission that showed apoptotic comet rate 3.0 %、 5.5 %, MBDI 1.1 %、 1.6 % respectively.Conclusion:Using comet assay,a large number of early stage apoptotic cells were detected easily in vivo in patients with AL during induction chemotherapy;Comet assay is a good method in predicting efficacy of chemotherapy.

目的 :观察急性白血病化疗后“凋亡彗星”的变化及其与临床疗效的关系。方法 :采用改良 Singh法 (改良碱性单细胞凝胶电泳 ,即“彗星试验”)检测 15例急性白血病患者化疗后 4、8、12、2 4、36和 72 h“凋亡彗星”的变化。结果 :化疗前“凋亡彗星”均 <0 .5 % ,化疗 8h开始增高 ,2 4h达高峰 ,“凋亡彗星”百分率 >98.0 %、骨髓白血病细胞减少指数 (MBDI) >6 5 .0 %的 11例经 1个疗程化疗完全缓解 ;“凋亡彗星”百分率为 95 .5 %、MBDI为18.5 %的 1例经 2个疗程化疗完全缓解 ;“凋亡彗星”百分率为 84.0 %、MBDI为 6 .1%的 1例经 2个疗程化疗部分缓解 ;“凋亡彗星”百分率为 3.0 %和 5 .5 %、MBDI为 1.1%和 1.6 %的 2例患者临床未缓解。结论 :应用“彗星试验”在急性白血病患者化疗过程中可检出大量早期凋亡细胞 ;“凋亡彗星”的检测是早期判断化疗疗效的良好指标。

BACKGROUND & AIM: This article exploved a modified method of comet assay from the Singh's alkaline comet assay and discussed the reliability of this method and the CASP software used to measure the DNA damage induced by UV light. MATERIAL AND METHODS: DNA damage of K562 cells induced by UV for different times (3 s,5 s,10 s,40 s,60 s,120 s,180 s,240 s) was measured by comet assay.The results were assayed by CASP. RESULTS: UV( 0.3 mW/cm2)induced a significant DNA damage (P<0.01) when the results were assayed by...

BACKGROUND & AIM: This article exploved a modified method of comet assay from the Singh's alkaline comet assay and discussed the reliability of this method and the CASP software used to measure the DNA damage induced by UV light. MATERIAL AND METHODS: DNA damage of K562 cells induced by UV for different times (3 s,5 s,10 s,40 s,60 s,120 s,180 s,240 s) was measured by comet assay.The results were assayed by CASP. RESULTS: UV( 0.3 mW/cm2)induced a significant DNA damage (P<0.01) when the results were assayed by the four parameters (tail length,comet length,tail moment,olive tail moment). CONCLUSION: The results suggested that the four parameters indicated a good time-dependent effect.The method which we developed and the four parameters were reliable.

背景与目的研究紫外线(Ultraviolet,UV)诱导K562细胞DNA损伤情况,评价彗星实验改良方法及分析参数的可靠性。材料与方法采用0.3mW/cm2UV以用不同时间(3、5、10、40、60、120、180、240s)照射K562细胞,诱导细胞DNA损伤,用彗星实验检测,CASP软件分析。结果尾长、彗星长、尾矩、Olive尾矩4个参数与紫外线照射时间有良好的时间依赖关系。结论0.3mW/cm2的紫外线照射K562细胞3s即可造成细胞DNA损伤,CASP分析软件可以用于彗星检测分析,其中尾长、彗星长、尾矩、Olive尾矩可作为彗星实验的分析指标,所改良的彗星实验系统可靠性较强,且基本上解决了实验中常见的脱胶现象,采图更方便。

The single cell gel electrophoresis or comet assay is now widely used to detect DNA damage in animal cells induced by radiation or chemicals.In the present study,the application of comet assay on UV-B induced DNA damage in the plant cell is reported.The standard protocol of the comet assay for the animal cell was modified for optimizing conditions for the plant cells.The specificity and sensitivity of comet assay on plant cell was improved by using protoplasts and T4 Endonuclease V.CASP software was used to...

The single cell gel electrophoresis or comet assay is now widely used to detect DNA damage in animal cells induced by radiation or chemicals.In the present study,the application of comet assay on UV-B induced DNA damage in the plant cell is reported.The standard protocol of the comet assay for the animal cell was modified for optimizing conditions for the plant cells.The specificity and sensitivity of comet assay on plant cell was improved by using protoplasts and T4 Endonuclease V.CASP software was used to analyze the comet image.Our results indicated that both UV-A and UV-B radiation can induce DNA single-strand breaking in the plant cell,however UV-B was more effective than UV-A for inducing pyrimidine dimers.

单细胞凝胶电泳(彗星检测)已广泛应用于动物细胞DNA损伤检测.该文报道了单细胞凝胶电泳应用于UV-B诱导植物细胞DNA损伤的检测.通过对动物细胞的单细胞凝胶电泳实验方法的改进,获得了在植物细胞中应用的最佳条件.以植物细胞原生质体为材料,并结合T4 Endonu lease V的运用,显著提高了UV-B诱导植物细胞DNA损伤提高彗星检测的敏感性和特异性.植物细胞DNA损伤的彗星图像经CASP软件处理并量化,结果表明:UV-A和UV-B均能诱导发生DNA单链断裂;UV-A在一定的剂量下诱导少量嘧啶二聚体的形成,而UV-B则强烈诱导嘧啶二聚体的产生.

 
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