Expression of Nucleocapsid Protein Gene of Porcine Reproductive and Respiratory Syndrome Virus in Soluble Form and Development of an Indirect ELISA for the Detection of Viral Antibodies with the Expression Product
Four primers were designed and the multiplex PCR were developed. Its sensitivity were CSFV 10 TCID50,PPV 10 TCID50,PRRSV 1 TCID50,PRV 100 TCID50 CSFV 10 TCID50,PPV 10 TCID50,PRRSV 1 TCID50,PRV 100 TCID50.The multiplex PCR for CSFV Shimen strain,CSFV lapinized virus strain,PRV Minnan A strain,PPV,PRRSV ky35 strain and B13 strain were done by this method.
Combined with burae tissue suspension of IBDV strain JD2, antigens were prepared with the allantoic liquid pooled from embryos of chickens and ducklings inoculated respectively with live virus strain ND LaSota, EDS76, IBD D78 and IBD isolates JD2. After inactivated with formalin, the antigens were mixed in suitable proportion immediately before addition of im-munopotentiator containing Se.
cyclotriazadisulfonamides), viral envelope gp120-binding agents such as plant lectins and glycopeptide antibiotics, HIV integrase inhibitors such as the pyranodipyrimidine V-165, and two new classes of compounds (i.e.
Both genes were determined to be nonessential in viral replication and infection.
Pseudorabies virus (PRV), an alpha-herpesvirus, has been developed as a live viral vector for animal vaccines.
Meanwhile, other findings reveal a relationship between host miRNA and viral infection.
These findings suggest a tight relationship between host and viral infection via miRNA pathway.
The coagulation kinetics of the dispersions of the A/Mississippi/1/85 influenza virus strain in NaCl solutions was studied at various pH values via the flow ultramicroscopy technique.
Common generalities inherent to the process of superfast coagulation of the A/Mississippi/1/85 influenza virus strain and strains previously studied are found.
A study was made of the coat protein (CP) of thermosensitive semidefective tobacco mosaic virus strain K1 (TMV-K1).
A mutation resulting in substitution of positively charged Lys53 with negatively charged Glu in the coat protein was introduced in the infectious cDNA copy of the genome of wild-type tobacco mosaic virus strain U1.
TP revealed fine structural differences between the wild-type tobacco mosaic virus (strain U1) and its temperature-sensitive mutant with an altered coat protein and host specificity.