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木质素过氧化物酶基因
相关语句
  lignin peroxidase genes
     TRANSCRIPTIONAL RESPONSIVENESS OF LIGNIN PEROXIDASE GENES TO NUTRITION IN Phanerochaete chrysosporium
     黄孢原毛平革菌木质素过氧化物酶基因对营养的转录应答
短句来源
  lignin peroxidase gene
     MOLECULAR CLONING AND ANALYSIS OF A LIGNIN PEROXIDASE GENE FROM PHANEROCHAETE CHRY SOSPORIUM
     黄孢平革霉(Phanerochaete chrysosporium)木质素过氧化物酶基因的克隆与分析
短句来源
     The medium and fermentation method of Pichia methanolica with lignin peroxidase gene(LipH8)of Phanerochaete chrysosporium was investigated. The optimum fermentation method of lignin peroxidase was studied by shake-flask culture. It is good that Pichia methanolica was cultured in the 11°Bx wort medium and then induced in the high temperature malt extract medium.
     对含有黄孢原毛平革菌木质素过氧化物酶基因(LipH8)的甲醇毕赤酵母培养基和发酵方法进行了研究.摇瓶培养条件研究结果表明:用11°Bx麦芽汁培养工程菌,然后用高温浸提液置换诱导产酶较好.5 L发酵罐产酶放大实验结果表明,木质素过氧化物酶较稳定,在72 h木质素过氧化物酶酶活达到最高7 568 U/L.
短句来源
  lip genes
     RT-PCR Analysis of Phanerochaete chrysosporium lip Genes in Colonized Fir Wood
     培养于天然冷杉木片的黄孢原毛平革菌木质素过氧化物酶基因表达的RT-PCR分析
短句来源
  “木质素过氧化物酶基因”译为未确定词的双语例句
     Expression of Phanerochaete chrysosporium lipA2(GLG3)、lipC1(GLG2)、lipC2(GLG5)、lipD2(GLG1)、lipE(LP0811) genes were analyzed by RT-PCR method. It was showed that some genes were expressed in special colonized period.
     利用RT PCR方法分析了生长于冷杉木片上的黄孢原毛平革菌木质素过氧化物酶基因lipA2 (GLG3)、lipC1 (GLG2 )、lipC2 (GLG5 )、lipD2 (GLG1 )、lipE(LP0 81 1 )的表达。
短句来源
     Cloning of cDNA Encoding Lignin Peroxidase (LipH8) from Phanerochaete chrysosporium and Expression in Pichia methanolica
     木质素过氧化物酶基因(LipH8)的克隆及在甲醇毕赤酵母中的表达
短句来源
     Analysis of 5′ Upstream Regulatory Sequences of Lignin Peroxidase( LIP ) Genes of Phanerochaete chrysosporium
     木质素过氧化物酶基因5'端上游调控序列的分析
短句来源
     Analysis of the sequences showed that there were many cis-regulatory elements in these DNA segments, implying that these sequences may be bound by some transcriptional regulation protein factors.
     对这些片段的DNA序列分析表明,它们均存在各种顺式作用元件,由此推测它们可能是被一些木质素过氧化物酶基因转录调控相关的蛋白质所结合的序列。
短句来源
     A genomic library of a filamentous white-rot fungus basidiomycete Phanerochaete chrgsosporium ME446 was constructed in the Bam HI site of Escherichia coli vector pUC 19 and screened with the lignin peroxidase(LIP)-encoding cDNAs:CLG 4 and CLG 5 isolated from strain BKM-F1767 as mixed probes. A positive clone,designated pGLG-M1,was obtained in this study.
     用大肠杆菌克隆载体pUC10构建白腐丝状担子真菌黄孢平革霉(Phanerochaetechrysosporium)ME446基因组文库,用分离自BKM-F1767菌株的木质素过氧化物酶cDNA片段CLG4和CLG5为探针,从构建的基因组文库中分离到一个含木质素过氧化物酶基因的重组子pGLG-M1。
短句来源
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      lignin peroxidase genes
    Screening of basidiomycetes for lignin peroxidase genes using a DNA probe
          
    PCR amplification of lignin peroxidase genes in white rot fungi
          
    The 3'-untranslated region conserved in certain lignin peroxidase genes is double underlined.
          
    The cloning of lignin peroxidase genes has also been reported by Asadaet al.
          
    Unfortunately, expression of lignin peroxidase genes in heterologous systems has proven to be problematic.
          
      lignin peroxidase gene
    Molecular analysis of a Bjerkandera adusta lignin peroxidase gene
          
    Comparison of these sequences reveals a lignin peroxidase gene structure consisting of 1,116 bp of protein-encoding DNA that is interrupted by four intervening sequences.
          
      lip genes
    All LIP genes, except LIP7, also encode an N-terminal signal sequence.
          
    These data indicate lipid-independent, highly flexible in vitro and in vivo expression of a large number of LIP genes, possibly reflecting broad lipolytic activity, which may contribute to the persistence and virulence of C.
          
    All the LiP-encoding genes were expressed on wood with predominance of Pr-lip3 transcript abundance, in particular on spruce wood chips, where also time-dependent expression of the multiple lip genes was observed.
          
    Further, the MnP and LiP genes fall within clearly defined clades discriminated by certain key residues.
          
    However, there is still considerable uncertainty about the exact number and structure of LiP genes.
          
    更多          


    A genomic library of a filamentous white-rot fungus basidiomycete Phanerochaete chrgsosporium ME446 was constructed in the Bam HI site of Escherichia coli vector pUC 19 and screened with the lignin peroxidase(LIP)-encoding cDNAs:CLG 4 and CLG 5 isolated from strain BKM-F1767 as mixed probes. A positive clone,designated pGLG-M1,was obtained in this study. Restriction mapping of the LIP-encoding fragment indicated that it had a restriction map very similar to that of of GLG2 which wsa isolated from another strain...

    A genomic library of a filamentous white-rot fungus basidiomycete Phanerochaete chrgsosporium ME446 was constructed in the Bam HI site of Escherichia coli vector pUC 19 and screened with the lignin peroxidase(LIP)-encoding cDNAs:CLG 4 and CLG 5 isolated from strain BKM-F1767 as mixed probes. A positive clone,designated pGLG-M1,was obtained in this study. Restriction mapping of the LIP-encoding fragment indicated that it had a restriction map very similar to that of of GLG2 which wsa isolated from another strain BKM-F1767.

    用大肠杆菌克隆载体pUC10构建白腐丝状担子真菌黄孢平革霉(Phanerochaetechrysosporium)ME446基因组文库,用分离自BKM-F1767菌株的木质素过氧化物酶cDNA片段CLG4和CLG5为探针,从构建的基因组文库中分离到一个含木质素过氧化物酶基因的重组子pGLG-M1。对该重组子插入片段所作的限制酶谱分析结果表明,它与来自BKM-F1767的GLG2片段的限制酶谱十分相似。

    The white rot fungi, Phanerochaete chrysosporium Burds can produce lignin peroxidase in degrade lignin and the model compounds, which expression is affected by various factors. By RT PCR, the transcription of GLG1, GLG2, GLG3 and GLG 5 genes were analyzed under the conditions of different nutrition combinations and air. It was showed that the four genes appeared in extensively different transcriptional responsiveness to different nitrogen...

    The white rot fungi, Phanerochaete chrysosporium Burds can produce lignin peroxidase in degrade lignin and the model compounds, which expression is affected by various factors. By RT PCR, the transcription of GLG1, GLG2, GLG3 and GLG 5 genes were analyzed under the conditions of different nutrition combinations and air. It was showed that the four genes appeared in extensively different transcriptional responsiveness to different nitrogen and carbon resources. However, the transcriptions of GLG 1 and GLG 5 genes did not occur in the experimental conditions. The transcriptions of GLG 2 and GLG 3 genes occurred in most nutrition combinations, but with great difference.

    白腐担子真菌黄孢原毛平革菌 (PhanerochaetechrysosporiumBurds)能产生降解木质素及其模式化合物的木质素过氧化物酶同工酶 ,其表达受诸多因素的影响 .利用反转录聚合酶链式反应 (RT PCR) ,分析了编码三个主要过氧化物酶同工酶H2 ,H8和H10的基因GLG1,GLG3和GLG2及其相关基因GLG5在 7种不同组合的氮、碳等营养培养基中和不充纯氧条件下的转录 .结果显示 ,所分析的 4个木质素过氧化物酶基因对不同碳氮组合的转录应答存在显著的差异 .在不同的 7种组合培养基中 ,都没有检测到GLG1和GLG5基因的转录产物 .GLG2和GLG3基因能够在一些营养组合条件下转录 ,但也存在一定的差异 .

    Expression of Phanerochaete chrysosporium lipA2(GLG3)、lipC1(GLG2)、lipC2(GLG5)、lipD2(GLG1)、lipE(LP0811) genes were analyzed by RT-PCR method. It was showed that some genes were expressed in special colonized period. Only lipA2(GLG3)transcription occured in the 2nd week and the 8th week, both lipC1(GLG2) and lipD2(GLG1)gene transcription was checked out in the period of 6 weeks. However, no lip genes were expressed in the time of 4 weeks. These results indicated that lip gene expression is relied on the colonized...

    Expression of Phanerochaete chrysosporium lipA2(GLG3)、lipC1(GLG2)、lipC2(GLG5)、lipD2(GLG1)、lipE(LP0811) genes were analyzed by RT-PCR method. It was showed that some genes were expressed in special colonized period. Only lipA2(GLG3)transcription occured in the 2nd week and the 8th week, both lipC1(GLG2) and lipD2(GLG1)gene transcription was checked out in the period of 6 weeks. However, no lip genes were expressed in the time of 4 weeks. These results indicated that lip gene expression is relied on the colonized period and transcript patterns are re dramatically different from those in previous studies with defined media.

    利用RT PCR方法分析了生长于冷杉木片上的黄孢原毛平革菌木质素过氧化物酶基因lipA2 (GLG3)、lipC1 (GLG2 )、lipC2 (GLG5 )、lipD2 (GLG1 )、lipE(LP0 81 1 )的表达。结果发现在不同的培养时间里仅有特定的基因表达 ,在第 2周时仅有lipA2 (GLG3)基因表达 ,在第 4周时未检测到任何基因的表达 ,在第 6周时lipD2 (GLG1 )和lipC1 (GLG2 )基因表达 ,在第 8周时仅有lipA2 (GLG3)基因表达。这些结果说明 ,在冷杉木片上培养的黄孢原毛平革菌的lip基因表达具有明显的时间特异性 ,并且与限定培养基中得到的结果明显不同。

     
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