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聚焦检测
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  focusing detection
     ALIGNMENT TECHNIQUE & FOCUSING DETECTION TECHNIQUE OF AN SUB-MICRON FIB SYSTEM
     亚微米聚焦离子束系统的合轴技术及聚焦检测技术
短句来源
  focus detecting
     A Study of Focus Detecting for the Head of Cutting Machine
     刻录头的聚焦检测
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  “聚焦检测”译为未确定词的双语例句
     DcR3 expression in COS-7 cells was verified by Western blotting and confocal microscopy.
     将重组子转染COS-7细胞,Western blotting和激光共聚焦检测到DcR3分子的表达。
短句来源
     Tamoxifen(10 -6 mol/L) also increased the releasing of calcium in Bcap-37 and HeLa cells.
     激光共聚焦检测到TAM( 10 -6mol/L)可使Bcap 37细胞和HeLa细胞内的Ca2 +浓度显著升高。
短句来源
     Discussion on focusing distance of ultrasonic focus detection
     超声波聚焦检测焦距的探讨
短句来源
     The apoptosis of HepG2 cells was induced by TNF-α and Act-D and then detected by the fluorescent cytometric way in 0,16th,32th,48th hour and by the laser cofocal way in 32th hour.
     以TNF-α、Αct-D诱导HepG2细胞株凋亡,0、16、32h和48h采用流式细胞术检测细胞凋亡,32h时采用共聚焦检测细胞凋亡比例。
短句来源
     Results: It was found that BH3-HIV-TAT peptide was mainly located in cell nucleus.
     结果:激光共聚焦检测结果显示BH3-HIV-TAT肽主要定位在细胞核内;
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  相似匹配句对
     Focus
     聚焦
短句来源
     A Study of Focus Detecting for the Head of Cutting Machine
     刻录头的聚焦检测
短句来源
     Discussion on focusing distance of ultrasonic focus detection
     超声波聚焦检测焦距的探讨
短句来源
     FOCUS
     热点聚焦
短句来源
     spectroscopy detection.
     能谱检测
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Human seminal plasma specific antigenp30.which has been characterized by otherauthors under the name of prostate-specificantigen or γ-seminoprotein, has beenporified. This communicaion describes thephysico-chemical characterization of p30demonstrated by various SDS-polyacrylami-de gel electrophoresis and isoelectrofocusingas well as amino acid analysis. The resultsshowed that the molecular weight of p30was 33 kd and its isoelectric point wasaround pH 6.9. Its amino acid compositionwas similar to that of prostate-specificantigen.It...

Human seminal plasma specific antigenp30.which has been characterized by otherauthors under the name of prostate-specificantigen or γ-seminoprotein, has beenporified. This communicaion describes thephysico-chemical characterization of p30demonstrated by various SDS-polyacrylami-de gel electrophoresis and isoelectrofocusingas well as amino acid analysis. The resultsshowed that the molecular weight of p30was 33 kd and its isoelectric point wasaround pH 6.9. Its amino acid compositionwas similar to that of prostate-specificantigen.It is concluded that p30 purified byour laboratory and prostate-specific antigennamed by Wang are the identical one ofisomers.

作者在分离纯化人类精浆特异性抗原p30的基础上,用不同凝胶浓度的SDS-PAGE检测该纯化p30的分子量,用等电聚焦检测其等电点,并分析其氨基酸组成。结果证明p30与前列腺特异性抗原属同一种糖蛋白。本文还探讨了不同作者所测p30理化参数不一致的原因。

The isolation and purification of thyrotropin (TSH) and gonadotropin (GTH) from the pituitary of Chinook Salmon were carried out by: (1). Alkaline extraction of acetone dried pituitary powder; (2). affinity chromtography of the pituitary extracts on Con A-Sepharose 4B; (3) filtration chromatography of glycoprotein on Ultro gel AcA 54; (4). cation exchange chromatography of fractions with thyrotropic and gonadotropic activities on carboxyme hyl (CM) Bio-gel A and (5). immunoaffinity chromatography of TSH active...

The isolation and purification of thyrotropin (TSH) and gonadotropin (GTH) from the pituitary of Chinook Salmon were carried out by: (1). Alkaline extraction of acetone dried pituitary powder; (2). affinity chromtography of the pituitary extracts on Con A-Sepharose 4B; (3) filtration chromatography of glycoprotein on Ultro gel AcA 54; (4). cation exchange chromatography of fractions with thyrotropic and gonadotropic activities on carboxyme hyl (CM) Bio-gel A and (5). immunoaffinity chromatography of TSH active fraction on immobili zed anti-beta SGTH antibody Sepharose 4B. The TSH molecule purified was homogenous in SDS-PAGE and exhibited high thyrotropic activity since this preparation stimulated thyroxine (T4) release in vivo in the homologous TSH bioassay. Gonadotropin (GTH), Somatotropin (GH) and prolactin (PRL) contaminations in the purified sTSH final preparation were very low. In an in vitro GTH bioassay, the sTSH exhibited no gonadotropic activity whereas the purified sGTH preparation exhibited high steroidogenic activities and maturational activities. Molecular weights were estimated by HPLC to be 27500 and 38000 dalton for sTSH and sGTH, respectively Isoelectric focusing showed that both sTSH and sGTH consisted of multiple components which differed in isoelectric points ranging between 5.4-7.0 for sTSH and between 3.5-5.5 for sGTH.

本文报道应用ConA-Sepharose 4B.亲和层析、凝胶过滤层析、离子交换层析及免疫亲和层析等技术,首次从大鳞大马哈鱼垂体中分离纯化了促甲状腺素(sTSH)和促性腺激素(sGTH)。在TSH生物测定中,纯化的。sTSH制品具有明显促进虹鳟幼鱼甲状腺体内分泌甲状腺素(T4)的生物活性,而sGTH无此活性。在GTH生物测定中,制备的sGTH具有显著诱导虹鳟卵母细胞体外培养成熟(GVBD)及分泌17α、20β-二氢孕酮的能力,而sTSH无此活性。HPLC表明sTSH和sGTH的分子量分别为27500和38000道尔顿。作者用SDS聚丙烯酰胺凝胶电泳(SDS-PAGE)和等电聚焦检测了激素的纯度及等电点,用RIA或ELISA方法测定了几种垂体激素在sTSH终产物中的污染程度。

A photothermal refraction experimental apparatus employing a glass capillary tube as the sample cell is developed for the determination of trace iron. Optimum signal-to-noise ratio is obtained by using a slit instead of a pinhole and focusing the probe beam. The detection limit of iron-phenanthroline is 4.0×10-8mol/L, corresponding to a absorbance of 4.0×10-6. This result is compared with other photothermal measured results, and a variety of possible ways to enhance the detection sensitivity are suggested.

以玻璃毛细管为样品池建立了测定痕量铁的光热折射光谱实验装置,选用狭缝取代针孔和采用探测束聚焦的检测方式,获得了较好的信噪比。测定铁一邻菲啉的检测下限为4.0×10-8mol/L,相当于4.0×10-6吸光度,并与其他光热测量结果相比,讨论了提高灵敏度的可能途径。

 
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