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诱导分化鉴定
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  “诱导分化鉴定”译为未确定词的双语例句
     Then, the neural stem cells were differentiated by 5% fetal bovine serum. The species and percent of the differentiated cells were examined to compare with those without Sapeptide. Finally, the differentiated cells were evaluated via immunohistochemistry stain by using connexin32 (CX32) and growth associated protein-43 (GAP-43).
     方法观察塑形的Sapeptide材料,将培养的脊髓神经干细胞接种其上,然后鉴定干细胞并通过体积分数5%胎牛血清诱导分化,鉴定分化细胞的类型及比例,并与无材料的干细胞的分化情况相比较,最后对材料上的分化细胞进行缝隙连接蛋白32(CX32)和生长相关蛋白43(GAP-43)染色评价。
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     Media were changed every 3 to 4 days.
     3、成骨诱导分化鉴定:细胞传代后,加入成骨诱导剂进行诱导。
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     [METHODS] BMSCs were identified by their morphology analysis and their capacities in the osteogenic、 chondrogenic and adipogenic differentiation.
     【方法】通过形态学观察及向成骨、成脂、成软骨定向诱导分化鉴定大鼠BMSCs。
短句来源
     Methods Culture the MSCs of Wistar rats, and then induce them with DMSO and BHA in vitro. Detect the specific marking proteins of neurons, glia and neural stem cells in the uninduced MSCs and induced MSCs. And observe their ultrastructure changes after the inducement.
     方法 培养大鼠MSCs,用二甲亚砜 (DMSO)和丁羟茴醚 (BHA)诱导分化 ,鉴定诱导分化前后的细胞是否表达神经细胞及神经干细胞的特异性标记蛋白 ,并研究其超微结构的变化。
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     Lower limb ischemia model was established, BMSCs amplified in vitro were labeled with fluorescence labeling and transplanted into left ischemic tissue, the same volume into the right ischemic tissue as control group. Three observation periods were 7, 14 and 28 days after transplantation respectively(n=6). Frozen slices were observed under microscope, and other slices were HE and vWF stained and observed to study time relationship between fluorescent positive cells and staining positive cells.
     体外诱导分化鉴定分离的细胞,建立下肢缺血模型,荧光标记的体外扩增的骨髓基质细胞被移植入左侧缺血组织作为移植组,右侧注射等量培养介质作为对照组,分为移植后第7,14,28天3个观察期(n=6),冷冻切片荧光显微镜观察,毗邻切片HE及vWF染色,检查荧光阳性细胞与染色阳性细胞的时空关系,并计算移植前后毛细血管密度的变化。
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     The cultured cells in vitro were induced with endothelial cell growth supplement.
     体外诱导分化鉴定分离的细胞。
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     2. Identification of the undifferentiated ESCs and neural induced cells.
     2.鉴定分化诱导分化后细胞
短句来源
     Purification,Induced Differentiation and Identification of Rat Embryonic Neural Stem Cells
     大鼠胚胎神经干细胞的纯化、诱导分化鉴定
短句来源
     The Induction and Differentiation of Neural Stem Cells
     神经干细胞的诱导分化
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     A Study on Isolation, Culture, Identification, and Induced in Vitro Differentiation of Dental Pulp Stem Cells
     牙髓干细胞分离培养鉴定和体外诱导分化的研究
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  differentiation and identification
Current research focuses more on understanding the mechanism of hair-cell regeneration/differentiation and identification of growth factors that can stimulate hair-cell regeneration.
      
The technique permits to prove different types of collagen in the eye, and allows the differentiation and identification of the tissues.
      
Origin, recruitment, differentiation and identification of osteoblasts, osteoclasts, stromal cells and cartilage cells
      
Origin, recuritment, differentiation and identification of osteoblasts, osteoclasts, stromal cells and cartilage cells
      
Analysis of whole-cell protein profiles was shown to be a relatively simple, easy, and reproducible procedure for the reliable and fast differentiation and identification of the enterococcal species.
      
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Objective To establish a method of isolating, culturing and identifying rat bone marrow mesenchymal stem cells (MSCs) in vitro and explore their biological properties in order to provide an experimental foundation for applying MSCs to trauma repair. Methods After the tibias and femurs were dissected from 5 to 6 week old rats, the marrow was flushed out with ice cold IMDM medium. The mononuclear cells of the marrow were obtained with density gradient centrifugation and then plated and cultured in IMDM...

Objective To establish a method of isolating, culturing and identifying rat bone marrow mesenchymal stem cells (MSCs) in vitro and explore their biological properties in order to provide an experimental foundation for applying MSCs to trauma repair. Methods After the tibias and femurs were dissected from 5 to 6 week old rats, the marrow was flushed out with ice cold IMDM medium. The mononuclear cells of the marrow were obtained with density gradient centrifugation and then plated and cultured in IMDM medium. The cultured cells in vitro were identified with their multipotential differentiation. And the growth curve, adhensive rate, cell cycle and ultrastructures of passaged cells were observed. Results The adherent, fibroblast shaped cells approached confluence in single layer 12~16 d after plating. The cultured MSCs in vitro differentiated into osteoblasts and lipoblasts. The double time of MSCs was about 38 h. More than 90% of subcultured MSCs were adhesive in 12 h. The cell cycle analysis showed that 80% of MSCs was in G0/G1 phase. The ultrastructures of MSCs demonstrated the features of infantile cells. Conclusion The subcultured MSCs possess multipotential differentiation. MSCs show stable growth in vitro , easy survival in the subculture and rapid proliferation in present culture condition. And MSCs also exhibit infantility in its ultrastructures and cell cycle. MSCs may be used in the healing of autogeneous trauma.

目的 建立一种体外分离、培养和鉴定小鼠骨髓间质干细胞的方法 ,探讨体外培养中间质干细胞的一些生物学特点 ,为利用MSCs促进创伤修复提供实验基础。方法 分离 5~ 6周龄的小鼠胫骨、股骨 ,用预冷的IMDM培养基冲洗出骨髓 ,经密度梯度离心得到骨髓单个核细胞 ,接种后 1 2~ 1 6d形成单层贴壁的成纤维状细胞。体外多向诱导分化鉴定分离的细胞 ,用传代的细胞进行生长曲线的测定、观察其接种贴壁率、检测细胞周期和超微结构。结果 体外传代培养的MSCs具有分化形成成骨和成脂细胞的能力 ;MSCs倍增时间约为 3 8h ,传代后 1 2h贴壁达 90 %以上 ,细胞周期显示有 80 %细胞处于G0 G1 期 ,并用电镜照片显示其超微结构。结论 在本实验条件下 ,体外培养的MSCs具有多向分化潜能 ,体外生长稳定 ,传代后的细胞适应性强 ,增殖较快 ,超微结构和细胞周期表现出较早期细胞特点。可望用于自体创伤促愈

Objective To establish a method of isolating, culturing and identifying mouse bone marrow stromal cells(MSCs) in vitro and vasculogenesis in vivo and explore their biological properties in order to provide an experimental foundation for applying MSCs to improve ischemia Methods After the tibias and femurs were dissected from 5 to 6 week old mice, the marrow was flushed out with ice cold DMEM/F12 medium The mononuclear cells of the marrow were obtained with density gradient centrifugation and the...

Objective To establish a method of isolating, culturing and identifying mouse bone marrow stromal cells(MSCs) in vitro and vasculogenesis in vivo and explore their biological properties in order to provide an experimental foundation for applying MSCs to improve ischemia Methods After the tibias and femurs were dissected from 5 to 6 week old mice, the marrow was flushed out with ice cold DMEM/F12 medium The mononuclear cells of the marrow were obtained with density gradient centrifugation and the plated and cultured in DMEM/F12 medium The cultured cells in vitro were identified with their potential differentiation, growth curve, adhesive rate, cleavage index, cell cycle, ultrastructure and vasculogenesis were observed Results The adherent fibroblast shaped cells approached confluence in single layer 12 to 16 d after plating The cultured MSCs in vitro differentiated into endothelium The double time of subcultured MSCs was about 42 h More than 90% of subcultured MSCs were adhesive in 10 h Cleavage index curve was similar with the growth curve The cell cycle analysis showed that about 83% of MSCs was in G 1 phase MSCs transplanted into ischemic tissues took part in vasculogenesis Conclusion The subcultured MSCs possess potential differentiation into vascular endothelium cells MSCs show stable growth in vitro, easy survival in the subculture and rapid proliferation in present culture condition MSCs may be able to be used in therapy of myocardium ischemia in the future

目的 探讨体外培养的骨髓基质细胞的一些生物学特性及体内移植后在缺血区新血管生成中的作用。方法 分离 5~ 6周龄的小鼠胫骨、股骨 ,用预冷的DMEM/F12培养基冲洗出骨髓 ,经密度梯度离心分离出骨髓单个核细胞 ,接种后 12~ 16d形成单层贴壁的成纤维样细胞。体外诱导分化鉴定分离的细胞 ,用传代的细胞进行生长曲线测定 ,观察其接种贴壁率、分裂指数 ,检测细胞周期和超微结构 ,并建立下肢缺血模型。荧光标记的体外扩增的骨髓单个核细胞被移植入缺血组织。移植后 2周 ,荧光显微镜及内皮细胞碱性磷酸酶染色 ,检查荧光阳性细胞与染色阳性细胞的时空关系。结果 体外传代培养的单个核细胞倍增时间约为 4 2h。传代 10h贴壁率达 90 %以上。分裂指数曲线与生长曲线相似。细胞周期显示约 83%的细胞处于G1期。结论 体外培养的骨髓基质细胞生长稳定 ,传代后的细胞适应性强 ,增殖较快 ,表现出较早期细胞特点 ,在体外及移植入体内缺血区能分化为血管内皮细胞 ,有望用于改善组织缺血

Objective:To establish a method for the isolation of porcine mesenchymal stem cells(MSCs) from bone marrow and to demonstrate their differentiation ex vivo into various mesenchymal tissue cells. Methods:MSCs were isolated from bone marrow and purified by centrifuge and in vitro.The proliferation and growth characteristics were observed in primary and passage culture.Cell cycle was analyzed by measuring DNA content with FACScan flow cytometer and cell multipotent was identified with specific staining....

Objective:To establish a method for the isolation of porcine mesenchymal stem cells(MSCs) from bone marrow and to demonstrate their differentiation ex vivo into various mesenchymal tissue cells. Methods:MSCs were isolated from bone marrow and purified by centrifuge and in vitro.The proliferation and growth characteristics were observed in primary and passage culture.Cell cycle was analyzed by measuring DNA content with FACScan flow cytometer and cell multipotent was identified with specific staining. Results:The adherent,fibroblast-like cells were confluent in single layer after plating for 12~14 days.The cultured MSCs in vitro differentiated into osteoblasts.The cell cycle analysis showed that 80% of MSCs were in G0/G1 phase.Conclusion:Porcine MSCs can be isolated from postnatal bone marrow through their adherent ability.Porcine MSCs may be introduced as a valuable model system to study the mesenchymal lineages for basic research and tissue engineering.

目的 :建立猪骨髓间质干细胞 (MSCs)的体外分离培养和鉴定的方法 ,探讨体外培养的间充质干细胞的一些生物学特点 ,为利用猪的实验研究提供实验基础。方法 :猪的髂嵴穿刺吸取骨髓 ,经密度梯度离心得到骨髓单个核细胞 ,接种后形成单层贴壁的成纤维样的细胞。检测细胞周期 ,多向诱导分化鉴定分离的细胞。结果 :体外培养的原代 MSCs 12~ 14 d达到融合 ,传代后仍具有分化成骨的能力 ,细胞周期显示有 80 %的细胞处于G0 / G1期。结论 :体外培养猪的 MSCs具有分化成骨的潜能 ,生长稳定 ,传代后仍保持未分化状态 .猪骨髓间充质干细胞分离培养体系的建立为基础研究和组织工程提供了一个有价值的动物模型。

 
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