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vero细胞毒
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  toxicity to vero cells
     Results New eha gene exited in one copy in some Et chromosomes. Moreover,the distribution of the product of pED102 for hemolysis and toxicity to Vero cells was main in the periplasmic compartment and cytoplasm.
     结果 新的迟缓爱德华氏菌 (Et)溶血活化基因以单拷贝形式存在于一部分Et菌染色体上 ,且pED10 2菌的Vero细胞毒和溶血活性作用主要位于胞浆蛋白和胞隙蛋白中。
短句来源
  vero cytotoxin
     A Comparison Study Between Multiplex PCR and Vero Cytotoxin Assays for Shiga Toxin-Producing Escherichia Coli
     产志贺毒素大肠杆菌多重PCR与Vero细胞毒实验对比研究
短句来源
     coli without stx 2 and stx 1 were negative in Vero cytotoxin assay.
     82株不含有 stx2 或 stx1 基因大肠杆菌 Vero细胞毒试验均阴性。
短句来源
  “vero细胞毒”译为未确定词的双语例句
     Development of Antigen Capture Indirect ELISA for Detecting IBDV in vero Cells
     抗原捕捉间接ELISA检测IBDV-vero细胞毒方法的建立
短句来源
     The productivity and activity of Stx2 through Stx2 preparations fromthe three E. coli O157:H7 strains carrying stx2::IS1203v were examined bythe HeLa and Vero cells assays and by measuring lactate dehydrogenase(LDH) release from HeLa and Vero cells as an indicator of cytotoxicity.
     此外,对这 3 株携带 stx2::IS1203v 的大肠杆菌 O157:H7 进行常规方法制备志贺毒素 2 并对其制备的产物进行 HeLa、Vero 细胞毒试验分析,将测定 HeLa、Vero 细胞乳酸脱氢酶释放量做为志贺毒素 2 细胞毒性的一个指示。
短句来源
     coli with stx 2 or stx 1 identified by multiplex PCR were positive, while 82 strains of E.
     [结果 ]6 0株多重 PCR stx2或 stx1 阳性大肠杆菌 Vero细胞毒试验均阳性。
短句来源
     In preparation of virus seed of HFRS vero cells vaccine,PS-6(TypeⅠ) and L99(TypeⅡ)strains of HFRSVs were continuously propagated on the vero cells,and the both strains were examined on virus titers,immunogenicity and passage stability.
     在肾综合征出血热(HFRS)疫苗Vero细胞毒种研制中,将肾综合征出血热(HFRS)病毒PS-6(Ⅰ型)、L99(Ⅱ型)在Vero细胞上连续传代,并对其病毒滴度、免疫原性、传代稳定性等进行检测。
短句来源
     Results The two kinds of IgY can neutralize the cytotoxin activity of Vero cells.
     结果  2种IgY抗体分别能够中和培养上清和菌体蛋白诱导的Vero细胞毒活性。
短句来源
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  相似匹配句对
     Cytochalasin B
     细胞B
短句来源
     Adaptation and passages of rabies virus strains in vero cell cultures
     狂犬病Vero细胞上的传代适应
短句来源
     Cytotoxic T Cell
     细胞T细胞
短句来源
     The Culture of Vero Cells and Babies Virus by A Domestic Cell Bioreactor
     用国产生物反应器培养Vero细胞和狂犬病
短句来源
     ADAPTATION AND PASSAGES OF CTN-1 STRAINS RABIES VIRUS IN VERO CELL CULTURES
     狂犬病CTN-1株在Vero细胞上的适应传代
短句来源
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  vero cytotoxin
Vero cytotoxin-producing strains of Escherichia coli in children with haemolytic uraemic syndrome and diarrhoea in Czechoslovaki
      
Isolation of vero cytotoxin-producingEscherichia coli serotypes O9ab:H- and O101:H-carrying VT2 variant gene sequences from a pa
      
Vero cytotoxin-producingEscherichia coli (VTEC) were isolated from the faecal specimen of a patient with haemolytic uraemic syndrome.
      
Seven persons who attended the Glastonbury Music Festival were infected with Vero cytotoxin-producing Escherichia coli O157 and an eighth person had serological evidence of infection.
      
Escherichia coli O157 strains from seven persons and from a cow belonging to a herd that had previously grazed the site all belonged to phage type 2 and possessed genes for Vero cytotoxin 2.
      
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After treatment 7 field isolates of infectious bursal disease(IBD) virus from six provinces inChina blindly passaged for three to four generation on Vero cell Iine,all of them could produce the CPE,Group specific anti infectious bursal disese virus(IBDV)monoclonal antibodies were chosen to detect Vero cell culture adapted isolates of IBDV at the 8th passage by indirect immunofluorescent assay(IFA ) and alkalinephosphatase antialkaline phosphatase complx bridge(APAAP)techniques,and the cell culture adapted...

After treatment 7 field isolates of infectious bursal disease(IBD) virus from six provinces inChina blindly passaged for three to four generation on Vero cell Iine,all of them could produce the CPE,Group specific anti infectious bursal disese virus(IBDV)monoclonal antibodies were chosen to detect Vero cell culture adapted isolates of IBDV at the 8th passage by indirect immunofluorescent assay(IFA ) and alkalinephosphatase antialkaline phosphatase complx bridge(APAAP)techniques,and the cell culture adapted iso-Iates at 1 4th the passage were further comfirmed by reverse transcription polymerase chain reaction (RT PCR).The results shwed that the tissue viruses of 7 field isolates all had adapted to the Vero cell Iine,this isfirst reported in China.

将国内6个省市(广东、巾东、河北、辽宁、、新疆和北京)的7株鸡传染性法氏囊病(IBD)的法氏囊组织处理后,直接在Vero细胞上盲传3~4代,均能不同程度的产生特征性细胞病变效应(CPE),并通过选用具有广谱性的抗法氏囊病病毒的单克隆抗体(IBI)V-McAb)采用间接免疫荧光(IFA)和碱性磷酸酶—抗碱性磷酸酶桥联酶显色技术(APAAP)的方法对7株分离毒的Vero第8代细胞进行鉴定,又用逆转—聚合酶链式反应(RT-PCR)对7株分离毒的第14代Vero细胞毒进行进一步证实,结果表明这7株IBD囊毒均适应于Vero细胞上,这是国内首次报道将组织毒直接适应于Vero传代细胞上。

An antigen capture indirect ELISA(ACI ELISA)for detecting viral titres in vero cells is developed.ACI ELISA and TCID50 are used for detecting X strain and N strain of IBDV in vero cell supernatant, the result showed that P/N values of ACI ELISA and TCID50 in vero cells had a good positive correlation (P<0.001).The correlation coefficient of ACI ELISA to X and N strains are 0.966 8,0.938, respectively.ACI ELISA can be used for detection of...

An antigen capture indirect ELISA(ACI ELISA)for detecting viral titres in vero cells is developed.ACI ELISA and TCID50 are used for detecting X strain and N strain of IBDV in vero cell supernatant, the result showed that P/N values of ACI ELISA and TCID50 in vero cells had a good positive correlation (P<0.001).The correlation coefficient of ACI ELISA to X and N strains are 0.966 8,0.938, respectively.ACI ELISA can be used for detection of IBDV in vero cells.

建立了检测用vero细胞增殖IBDVX、N毒滴度的抗原捕捉间接ELISA法(ACI-ELISA),并与常规TCID50测定法作了比较。结果表明,ACI-ELISA法测定的P/N值与TCID50呈明显的正相关,与X、N毒的相关系数分别为0.9668(P<0.001),0.938(P<0.001)。ACI-ELISA法可用于IBDV-vero细胞毒的定量监测。

Aim Explore the functions of the hemolysin activator (eha) gene of Edwardsiella tarda.Mathods The eha gene probe enlarged by PCR was labelled with Digoxigenin and was hybridized in situ and Southern blot with 26 strains Edwardsiella tarda.Results New eha gene exited in one copy in some Et chromosomes.Moreover,the distribution of the product of pED102 for hemolysis and toxicity to Vero cells was main in the periplasmic compartment and cytoplasm.Recombination plasmid pED102 with eha gene entered nonhemolytic...

Aim Explore the functions of the hemolysin activator (eha) gene of Edwardsiella tarda.Mathods The eha gene probe enlarged by PCR was labelled with Digoxigenin and was hybridized in situ and Southern blot with 26 strains Edwardsiella tarda.Results New eha gene exited in one copy in some Et chromosomes.Moreover,the distribution of the product of pED102 for hemolysis and toxicity to Vero cells was main in the periplasmic compartment and cytoplasm.Recombination plasmid pED102 with eha gene entered nonhemolytic Et and Escherichia coli(LE392?JM109)by electroporation,then no hemolytic phenomenon of these Et and hemolytic phenomenon of Escherichia coli were observed.Conclusion eha gene wasn't the hemolysin gene and was the hemolysin activator (eha) gene of Edwardsiella tarda.

目的 探讨新的迟缓爱德华氏菌溶血活化基因 (eha)的功能。方法 PCR扩增部分溶血活化基因 ,制成地高辛探针和 2 6株Et菌进行斑点杂交和Southernblot。结果 新的迟缓爱德华氏菌 (Et)溶血活化基因以单拷贝形式存在于一部分Et菌染色体上 ,且pED10 2菌的Vero细胞毒和溶血活性作用主要位于胞浆蛋白和胞隙蛋白中。将有溶血活化基因的 pED10 2重组质粒电击入不溶血的Et菌和大肠杆菌 (LE3 92、JM 10 9) ,Et菌仍无溶血现象 ,大肠杆菌有溶血现象。结论 eha基因不是溶血素结构基因 ,而是溶血活化基因

 
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