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The disease severity of bacterial blight decreased after sprayed with nickel nitrate solution in rice seedilings. According to a plate test,nickel nitrate was slightly toxic to Xanthomonas oryzae pv.oryzae,so the decrease in disease index of bacterial blight was mainly due to induction resistance to X.oryzae pv.oryzae,by nickel nitrate in rice seedlings. The concentration of nickel nitrate ranging from 0.55 to 2.75 mmol/L was effective, of which,2.2 mmol/L had the optimum induction. Rice seedlings displayed... The disease severity of bacterial blight decreased after sprayed with nickel nitrate solution in rice seedilings. According to a plate test,nickel nitrate was slightly toxic to Xanthomonas oryzae pv.oryzae,so the decrease in disease index of bacterial blight was mainly due to induction resistance to X.oryzae pv.oryzae,by nickel nitrate in rice seedlings. The concentration of nickel nitrate ranging from 0.55 to 2.75 mmol/L was effective, of which,2.2 mmol/L had the optimum induction. Rice seedlings displayed the strongest induction resistance to X.oryzae pv.oryzae when challenge inoculated with X.oryzae pv.oryzae 76 25 strain (high virulent) 3 days after the treatment of spraying the parts under the third leaf with 2.2 mmol/L nickel nitrate. The lesion length of pre treated rice leaves was 69.6% shorter than that of the control. On the 4th day after the treatment,the 3rd leaf of the crop was challege inoculated,from which the leaf acquired systemic resistance to bacterial blight.Its relative induction effect was 42.9%. The resistance effect induced by nickel nitrate lasted about 21 days. 硝酸镍溶液喷施处理后 ,水稻幼苗的白叶枯病发病程度降低 .经平板测定 ,硝酸镍对稻白叶枯菌只有轻微的直接毒性 ,表明水稻幼苗白叶枯病病情减弱 ,主要由于硝酸镍诱导了水稻幼苗对白叶枯病的抗性 .0 .0 5~2 .75 mmol/ L的硝酸镍均有明显的诱抗作用 ,其中 2 .2 m mol/ L为最佳诱导浓度 .2 .2 m mol/ L硝酸镍喷施处理后 ,水稻幼苗叶片病斑长比对照降低了 6 9.6 % .处理后第 3d水稻幼苗表现出最大的诱导抗性 .用 2 .2 mm ol/ L喷雾处理第 3叶以下部位 ,第 4d挑战接种第 3叶 ,使第 3叶产生了对白叶枯病的系统抗性 ,相对诱导效果达 42 .9% .硝酸镍的诱导抗病作用持续 2 1 d左右 Salicylic acid (SA), K2HPO4, filtrates of Cercospora zeae-maydis and oxalic acid (OA) were used to inducing the resistance of maize to gray leaf spot of maize (GLS) and searched changes of activities of phenylalanine ammonia lyase (PAL) which associated with the induced resistance among the four kinds of inducer. SA was the best inducer the filtrates of C. zeae-maydis and K2HPO4 were next, and after the weakest OA was the last one. PAL activities were low and the changes were stable and reached the peak value... Salicylic acid (SA), K2HPO4, filtrates of Cercospora zeae-maydis and oxalic acid (OA) were used to inducing the resistance of maize to gray leaf spot of maize (GLS) and searched changes of activities of phenylalanine ammonia lyase (PAL) which associated with the induced resistance among the four kinds of inducer. SA was the best inducer the filtrates of C. zeae-maydis and K2HPO4 were next, and after the weakest OA was the last one. PAL activities were low and the changes were stable and reached the peak value in 8 days after treatment by inducer. 用水杨酸、磷酸氢二钾、灰斑病菌滤液和草酸作诱抗剂 ,研究了玉米对灰斑病的诱导抗病作用 ,同时测定了挑战接种前后的PAL酶活性的变化。结果表明 :上述4种诱抗剂均有不同程度的诱导抗病性 ,其中水杨酸诱抗效果最好 ,病菌培养滤液和磷酸氢二钾次之 ,草酸诱抗效果最差。诱导后挑战接种 ,PAL酶活性变化都有增加 ,且酶活性曲线波形变化较大 Resistance effect induced by salicylic acid (SA), p- aminophenol (p-AP) ,nickel nitrate, paraquat (PQ), cupric chloride and Cu reagent in rice seedlings to Xanthomonas oryzae pv. oryzae (XOO) was compared. The optimum induction conc entrations and optimum induction phase of these factors were determined. Their o ptimum induction concentrations were 0.05mmol/L, 0.5mmol/L, 2.2mmol/L, 0.15mg/L, 0.3mmol/L and 0.5mmol/L respectively. SA and PQ displayed the most effective in duction effect when the pre-treated... Resistance effect induced by salicylic acid (SA), p- aminophenol (p-AP) ,nickel nitrate, paraquat (PQ), cupric chloride and Cu reagent in rice seedlings to Xanthomonas oryzae pv. oryzae (XOO) was compared. The optimum induction conc entrations and optimum induction phase of these factors were determined. Their o ptimum induction concentrations were 0.05mmol/L, 0.5mmol/L, 2.2mmol/L, 0.15mg/L, 0.3mmol/L and 0.5mmol/L respectively. SA and PQ displayed the most effective in duction effect when the pre-treated rice seedlings were inoculated with XOO 2 an d 4 days later respectively, while the other factors that the 3rd day after tr eatment. The local relative induction effect of SA, p-AP, nickel nitrate, PQ , Cupric acid and Cu reagent was 19.7%、34.4% 、69.6%、46.1%、12.3% and 18.9% resp ectively at their optimum induction concentrations. Obviously effective systemic resistance was induced by nickel nitrate, PQ and p-AP. On the 4th day after tre atment, they displayed the most significant induction effect of resistance to XO O systemically. The severity of bacterial blight was reduced by 40.9%、35.5% and 27.6% respectively. Moreover, resistance induced by them was still relatively significant on the 8th day after treatment. Comparatively, nickel nitrate was th e best chemical inducer. 比较了水杨酸 (SA) ,对氨基苯酚 (p -AP)、硝酸镍、百草枯 (PQ)、氧化铜和铜试剂对水稻幼苗抗白叶枯病的诱导抗病作用、它们的最佳诱导浓度和最佳诱导时期 .它们的最佳诱导浓度分别为 0 .0 5mmol/L、0 .5mmol/L、2 .2mmol/L、0 .1 5mg/L、0 .3mmol/L和 0 .5mmol/L .除SA和PQ的最佳诱导期分别为处理后第 2天和第 4天外 ,其余因子均在处理后第 3天 .在最佳浓度下 ,SA、p -AP、硝酸镍、PQ、氯化铜和铜试剂的相对诱导效果 (局部抗性 )分别为 1 9.7%、3 9.5%、69.6%、4 6.1 %、1 2 .3 %和 1 8.9% .硝酸镍、PQ和P -AP均有明显的系统诱导抗病作用 .三种因子均在处理后第 4天表现出最大的系统诱导抗病效果 ,相对诱导效果分别为 4 2 .9%、3 5.5%和 2 7.6% .并且 ,在处理后第 8天这种诱导作用也较明显 .
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