L3,L5,L6 serial section of spinal cord of each group was taken out separately and was made into 20 μm frozen sections. Then the sections were stained under the same condition by using specific c jun(1∶200) antibody by the immunohistochemical ABC method.
The stereotactic coordinates of FN were decided. The size of FN wasmeasured by Nissl's stained serial sections. RESULTS: Thestereotactic coordinates of FN in guinea pigs weighing 300 g~650 g were as followings: P: (2. 4 0. 1 ) mm, LL/RL: (2. 0 0. 07) mm, H: (- 1. 9 0. 08) mm.
In order to accelerate immunofluorescence histochemical staining (Indirect and Direct), microwave irradiation method was used and compared with routine incubation method in demonstrating 15 different tissue antigens on serial sections.
Methods：The density of apoptotic cell(DAC) of the brachial plexus,thoracic and lumbar plexus of spinal cord (SC)and spinal ganglion (SG)in the serial sections of fetal ICR mice aged 12～15.5 days was measured with the stereological method.
方法：以体视学方法观察E12d～E15．5d ICR胎鼠H．E染色石蜡连续切片中臂丛段、胸段、腰从段的SC和SG的凋亡细胞密度（Density of apoptotic cell，DAC）。
This paper introduces the progress in the technique of computer for three dimensional reconstruction from biological tissue series slices images. Emphasis is laid on registration, reconstruction & display,image data compressing and image automatic segmentation.
This method includes three steps,firstly,making series slices of biological tissue,secondly,capturing the pictures of the series slices by CCD camera,and then storing the pictures in sequence to a computer,finally,reconstructing the three dimensional images from the sequential pictures by the computer.
METHODS:The mouse embryos were taken at E 9.0 d,E 9.5 d,and E 10.5 drespectively,and were performed by preparation for paraffin series section using the staining of terminal deoxynucleotidyl transferase mediated dUTP biotin nick end labeling(TUNEL).
The SD rats were categorized in2groups:Sham operated groups(SOG)and Operated groups(OG),which were classified on1,2,4,7,14,21,30,45,60days afteroperaˉtion. The morphometry of the cardiac,pulmonary and renal arterioles was studied microscopically on series section of cardiac,pulmonary and renal tissure combining computer_assistant image analysis.
CP embedding agent and its application to the serial section of the body wall tissue of insects
It solves the 3 difficult problems in the serial section of the organ and tissue of insect, namely stiff materials, thin section and continuity of sections.
Using serial section electron tomographic methods, we have constructed accurate 3D models of the nodal complex from mouse spinal roots with resolution better than 7.5nm.
All p53-positive cases showed a concomitant immunohistochemical mdm-2 expression; an immunohistochemical colocalization on serial section was demonstrated in 12 anaplastic carcinomas.
Three-dimensional scale models constructed on the basis of serial section electron microscopy showed that only a little fraction of the mitochondria in the cells ofChlamydomonas reinhardii has a simple spherical or elliptical shape.