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  “5'上游区”译为未确定词的双语例句
     We first characterized 5′flanking promoter in the mouse embryonic carcinoma (EC) P19 cells. Primerextension showed that mouse nestin mRNA was transcribed from at least threedifferent initiation sites.
     利用小鼠P19胚胎性癌细胞(Embryonal carcinoma, EC),我们详细分析了小鼠Nestin基因5'上游区的启动子序列。
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     5).
     5).
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     heat preservation time (t)5h;
     t为5h。
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     SOIL EROSION ON SLOPE LAND AND EROSION CONTROL IN THE UPPER REACHES REGION OF THE YANGTZE RIVER
     长江上游区的坡面土壤流失及侵蚀防治
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     The soll and Water Erosion and Soil and Water Conseration in the Upper and Middle Reaches of Four Rivers in Hunan Province
     湖南四水中上游区水土流失与水土保持
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     The Preliminary Study of Information & Forecast System about Landslides & Debris Flows in Up-reach of Jingling River
     嘉陵江上游区滑坡泥石流预警信息系统初步研究
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  5 ' upstream region
In our preceding studies, we have identified microsatellite polymorphisms inside the PSMA6 gene and in its 5' upstream region.
      
The OPBP1 interacted specificallyin vivo with FA, a DNA sequence from the 5 upstream region of osmotin gene, which was essential for osmotin responsiveness.
      
The results suggest that the 5' upstream region flanking the coding sequence of thericMT may contain a fairly strong promoter.
      
Computer analysis of the sequence homology showed that the 5' upstream region included a putative TATA box, a putative CAAT box, and a typical metal-responsive element TGCGCGCG.
      
Therefore, the regulatory sequence conveying responsiveness of the PNMT gene to nicotinic stimuli has been characterized in the 5' upstream region of the rat PNMT promoter.
      
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This paper presents devices, measuring equipments, data processing and its major experimental results for turbulent boundary and boundary layer/ shock wave interaction in 2-D flate plate at Mach number 2.048. Cross-sectional area of wind tunnel test section is 0.6×0.6 m2, Reynolds number is 2.1×107 per metre and Reynolds number Res0 which .take boundary layer thickness of shock wave interaction region as reference length is approximately equal to 106.Experimental results of velocity profile u/u, for turbulent...

This paper presents devices, measuring equipments, data processing and its major experimental results for turbulent boundary and boundary layer/ shock wave interaction in 2-D flate plate at Mach number 2.048. Cross-sectional area of wind tunnel test section is 0.6×0.6 m2, Reynolds number is 2.1×107 per metre and Reynolds number Res0 which .take boundary layer thickness of shock wave interaction region as reference length is approximately equal to 106.Experimental results of velocity profile u/u, for turbulent boundary layer in 2-D flate plate are approaching to(h/δ)1/7 , and relatively agree well with numerical results of finite difference solutions for 2-D compressed boundary layer equations. The qualitative distributed rules for dynamic eddy viscosity coefficient εm along flow lines which are derived from experimental results in boundary layer/shock wave interaction are also presented in this paper.

本文介绍了M=2.048的二元平板紊流附面层和附面层与激波(激波板相对气流为8.9°)干扰实验的设备、测试仪器、数据处理以及主要的实验结果。实验用的风洞试验段截面积为0.6×0.6米~2,每米长雷诺数月Re=2.1×10~7,以激波干扰区附面层厚度为参考长度的雷诺数Reδ_0)≈10~6。 二元平板紊流附面层速度型的实验结果接近于u/ue=(h/δ)~(1/7),而且与二元可压缩附面层方程有限差分解的数值结果符合较好。实验结果与二元平板不可压附面层壁面定律和尾迹定律公式计算值的变化规律接近,但计算结果偏大些,而与速度亏损定律的结论则是一致的。 文中亦给出了由实验结果推算的激波附面层干扰时动涡粘系数ε_m沿流线的定性分布规律:在附面层内层区域,ε_m通过干扰区是逐渐增大的,至干扰区下游时恢复到某一大干上游区的ε_m值。而在外层区域,ε_m在干扰区是先减小后再增大,这时下游区的ε_m略大于上游区的ε_m值。

In the previous study (Cancer Res. 47, 3195, 1987) we have found three Bam H 1 digested fragments, namely 8.8 kb, 6.6 kb and 2.5 kb, from the clone A 120, which contains the c-Ha ras oncogene of human gastric carcinoma cell line BGC-823. The 6.6 kb fragment has been cloned and a part of its sequence was analyzed. The 2.5 kb fragment, which contains the Alu Sequence, was found at the upstream of the 6.6 kb fragment, which does not contain the Alu sequence, In the present study both the 2.5 kb and the 6.6 kb fragments...

In the previous study (Cancer Res. 47, 3195, 1987) we have found three Bam H 1 digested fragments, namely 8.8 kb, 6.6 kb and 2.5 kb, from the clone A 120, which contains the c-Ha ras oncogene of human gastric carcinoma cell line BGC-823. The 6.6 kb fragment has been cloned and a part of its sequence was analyzed. The 2.5 kb fragment, which contains the Alu Sequence, was found at the upstream of the 6.6 kb fragment, which does not contain the Alu sequence, In the present study both the 2.5 kb and the 6.6 kb fragments were labeled with 32P-dCTP and were used as probes to detect the DNA-binding nuclear proteins of the Rat 3-3 transformed cells and the Rat 1. untransformed cells.Both the Western and Southern blottings and also the gel shift techniques showed that only in the Rat 3-3 transformed cells there was a nuclear protein fraction strongly bound to the 2.5 kb DNA, but not to the 6.6 kb DNA. The Mr. of this nuclear protein is about 35 KD. The c-Ha-ras in the Rat 3-3 cell nuclei was hypersensitive to DNase I at concentration of 1 μg/mL, while that in the Rat 1 cell nuclei was not degraded by DNase I even at concentration of 15 μg/ml. In the Northern blotting for mRNAs extracted from Rat 3-3 and Ret 1 cells, by using 6.6 kb DNA as a probe, it was found that the expression of Ha-ras in the former was five times higher than the latter From the results shown above it seems that the 2.5 kb stretch has a function to regulate the expression of c-Ha-ras by binding to the 35 KD protein, thus enhancing the transcription of the activated c-Ha-ras oncogene.

以前的工作曾用人胃癌基因Ha-ras转化了大鼠全胚细胞系Ratl细胞,得到转化细胞Rat3-3。克隆了Ha-ras癌基因6.6kb及其上游区2.5kb DNA片段,并发现2.5kb有Alu重复顺序,说明这个片段是来源于人胃癌细胞,虽族观察到p21蛋白编码12位点突变,我们又发现转化的Rat3-3细胞的Ha-ras mRNA水平比未转化的Rat1高大约五倍;通过DNase I超敏感实验证明只有转化细胞核中的Ha-ras基因对DNaseI敏感,1μg/mL的DNaseI就有明显的降解,而未转化细胞Rat1细胞核的Ha-ras基因在15μg/mL的DNaseI中也未发现有任何降解;另外还发现转化细胞核有一种能为Ha-ras基因上游区2.5kb特异结合的核蛋白,分子量大约35kD,此核蛋白不能与6.6kb Ha-ras基因本身结合,在未转化细胞中未发现此蛋白。从这些结果推测,癌基因Ha-ras的活化,除了点突变外,还可能存在另一条活化途径,即它的上游区可能有类似增强子的调控区。

A new plasmid was ob(?)ained from pMCP1000 treated with restricted endonucleasc EcoRI, modified by enzyme Pal 31 and linked by T_4 DNA ligase. It was named as pAY series. A short and active alkA Gore p(?), pAY 108, was selected out by EMB plate culture, treatment with E coRI, Sal I and BamH I, and polyacrylaminde electrophoresis. sequence analysis of alkA Gene promoter showed the same from -49 upstrcam to initiator codon as reported by Nakabeppu in 1984, and the β-galactesidase activity was similar to wild type....

A new plasmid was ob(?)ained from pMCP1000 treated with restricted endonucleasc EcoRI, modified by enzyme Pal 31 and linked by T_4 DNA ligase. It was named as pAY series. A short and active alkA Gore p(?), pAY 108, was selected out by EMB plate culture, treatment with E coRI, Sal I and BamH I, and polyacrylaminde electrophoresis. sequence analysis of alkA Gene promoter showed the same from -49 upstrcam to initiator codon as reported by Nakabeppu in 1984, and the β-galactesidase activity was similar to wild type.

含有alkA基因启动子的质粒pMCP1000经用限制性内切酶EcoRI酶切、酶Ba131修饰和T_4DNA连接酶连接,重组成新质粒,命名为pAY系列。EMB琼脂板培养筛选,限制性内切酶EcoRI、SalI和BamHI酶切和聚丙烯酰胺凝胶电泳分离出最短的有活性的alkA基因启动子,pAY108。DNA序列分析证明在启动子上游区-49到起始密码区与1984年Nakabeppu报道的alkA基因启动子序列一致。β-半乳糖苷酶试验证明与野生型活性近似。

 
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