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In three of the cell lines, C5 was detected in both culture supernatants and whole cell detergent lysates, whereas in the other five cell lines, C5 was detected only in the cell lysates.
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garinii) and ELISAs with whole-cell detergent extracts of strains PKo, PKa2 (B.
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The sensitivities of the three whole-cell detergent-extract ELISAs were similar (46.6 - 49.2%), whereas those of the recombinant ELISAs were highest with p41/i:PBi (57.1%) and lowest with p41/i:PKo (21.5%).
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The correlation of absorbance values of different assays was very high for the three whole-cell detergent-extract ELISAs, whereas the absorbance values obtained with the three recombinant tests differed considerably.
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Whole cell detergent-soluble extracts of untransfected 293 cells were included as a negative control.
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| | Objective To compare the effect of fabricating decellularized scaffold of homograft bioprosthetic tube valved (HBTV) with two kinds of cell detergents and to provide a homograft bioprosthetic scaffold for fabrication of tissue-engineering heart valve (TEHV). Methods The active cells in the HBTV, which conserved by liquid nitrogen, were decellularized by low osmotic pressure of Tris buffer, in which containing sodium dodecylsulphate (SDS) and deoxycholic acid (DOA) respectively. The leaflets or aortic wal... | | 目的 比较去氧胆酸钠(deoxycholic acid,DOA)和十二烷基硫酸钠(sodium dodecylsulphate,SDS)两种细胞洗涤剂制备脱细胞的同种生物带瓣管道(homograft bioprosthetic tube valved,HBTV)支架的效果,为体外构建组织工程瓣(tissue- engineering heart valve,TEHV)提供同种生物瓣支架材料。 方法 对同种带瓣主动脉管道和带瓣肺动脉管道,分别用含0 .5 % DOA或0 .0 3% SDS的Tris低渗缓冲液共同孵育4 8h,脱去经液氮保存、具有活性的HBTV表面的内皮细胞,固定剂固定后分别进行HE、胶原纤维和弹力纤维染色的光镜观察,以及扫描电镜观察,摄片。 结果 两种洗涤剂均完全地脱去了HBTV表面的内皮细胞,中心部位的成纤维细胞有部分存留;细胞外基质,如胶原纤维和弹力纤维等均较完整地保留下来,其形态结构与脱细胞前无明显改变。 结论 0 .0 3%~0 .1% SDS或0 .5 % DOA的低渗缓冲液对制备HBTV... | | 文摘来源 | |   |
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