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秆形变异
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     STUDIES ON THE STRUCTURE OF CULM FORM OF PHYLLOSTACHYS PUBESCENS
     毛竹结构的研究
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     Study on the Structure of Culm Form of Bambusa wenchouensis
     大木竹结构的研究
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     Current Research on Bamboo Culm Form
     竹子生长和变异的研究进展
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     Shape * Shadow
     *影
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     The Effect of Management on the Growing and Form of Moso Bamboo Culms
     人工经营对毛竹结构变异的影响
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The total RNA was extracted from the bamboo shoots of normal and abnormal internode of Bambusa ventricosa respectively. After mRNA was purified,cDNA libraries were constructed with mRNA using Uni-ZAP XR vector. The cDNA libraries of normal and abnormal internode consisted of 7.4×109 pfu·mL-1 and 8.7×109 pfu·mL-1 respectively. Both cDNA libraries contained over 99% recombinant phages,in which inserts ranged between 500bp and 3 000 bp in sizes. The successful construction of the cDNA libraries has established...

The total RNA was extracted from the bamboo shoots of normal and abnormal internode of Bambusa ventricosa respectively. After mRNA was purified,cDNA libraries were constructed with mRNA using Uni-ZAP XR vector. The cDNA libraries of normal and abnormal internode consisted of 7.4×109 pfu·mL-1 and 8.7×109 pfu·mL-1 respectively. Both cDNA libraries contained over 99% recombinant phages,in which inserts ranged between 500bp and 3 000 bp in sizes. The successful construction of the cDNA libraries has established a base for the study on the molecular mechanism of internodal elongation in Bambusa ventricosa.

以同一丛小佛肚竹新萌生的正常杆笋尖和畸形杆笋尖为材料,分别提取总RNA,经纯化成mRNA后,合成cDNA双链,以Uni-ZAPXRvector为载体,构建了两个cDNA文库。正常杆笋和畸形杆笋cDNA文库的滴度分别为7.4×109pfu·mL-1和8.7×109pfu·mL-1,含插入片段的频率均达到是99%以上,插入片段的大小均在500~3000bp。这两个cDNA文库的成功构建为探究小佛肚竹秆形变异的分子机理奠定了基础。

 
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