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Sandhya Gupta optimized vitrification and encapsulation cryopreservation protocols for several accessions of Rubus.
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| | The researches on conservation of conchocelies germplasm of Porphyra haitanensis were done.Three different kinds of conservation methods(Liquid conservation,solid conservation and encapsulation cryopreservation) were compared.The result showed: 1) The weights of conchocelies germplasm increased by-0.67% during the 30 days under the condition of temperature 5 ℃ and they could develop normally up to 120 days without changing culture medium under the temperature 10 ℃ when the method of liquid conservation was ... | | 采用液体培养基、固体培养基以及胶囊化冷冻法保存坛紫菜丝状体种质,探讨了不同保存条件对其生存的影响。结果表明:液体培养基保存丝状体,在低温5℃,培育30d时,藻丝的增重率为-0.67%;温度为10℃,有利于延长更换培养液的时间,即使120d不换培养液,丝状体也能正常生长。固体培养基保存丝状体,在5℃时固体平板上的细菌生长慢、数量少,但藻丝无法适应低温条件,逐渐死亡;丝状体在10℃固体平板上生长缓慢,但保存100d以上仍然正常生长,达到了长期保存的目的。在胶囊化低温保存中,丝状体存活率较高,而且随着保存时间的延长,成活率没有显著的变化,保持在75%以上。 | | 文摘来源 | |   |
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