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A plasmid carrying lambda PL promoter was constructed to efficiently express unfused human interferon αD in E.Coli using TGATG site per sc in its signal sequence.The unfused human interferon aD expressed by pBV867 was confirmed by SDS-PAGE,activity assay and N terminal sequencing.Results suggested that E.Coli is able to process the signal sequence of human interferon αD.It was also demonstrated that the expression level by ATG-TGATG initiation mode was 10 times higher than that by single ATG mode,and the PV40...

A plasmid carrying lambda PL promoter was constructed to efficiently express unfused human interferon αD in E.Coli using TGATG site per sc in its signal sequence.The unfused human interferon aD expressed by pBV867 was confirmed by SDS-PAGE,activity assay and N terminal sequencing.Results suggested that E.Coli is able to process the signal sequence of human interferon αD.It was also demonstrated that the expression level by ATG-TGATG initiation mode was 10 times higher than that by single ATG mode,and the PV40 DNA Hind 1 B fragment can enhance the expression of human interferon αD in E.Coli.

本文利用人αD型干扰素基因信号多肽编码区内自身的TGATG序列,在大肠杆菌中高效表达非融合的人αD型干扰素。发现“翻译联结”(ATG-TGATG)方式起始的基因表达水平比一般ATG起始方式高约10倍;证明大肠杆菌能够识别并加工人αD型干扰素信号多肽。发现SV_(40) DNA HindⅢ B片段对人αD型干扰素基因在原核细胞中表达有增强作用。

Signals of left ventricular pressure,carotid arterial pressure and

用心血管功能程序,将左心室压、动脉压和升主动脉流量3个生理信号经微机运算后获得29个心血管生理参数,证明此程序在心血管功能研究中较为理想。电针家兔内关穴区时,由于刺激参数不同,可引起降压或升压反应,在升压或降压中,副交感神经均处于抑制状态,提示副交感神经系统的抑制状态可能与针刺作用有关。针刺内关穴区时,特别在升压反应中,交感神经处于兴奋状态,可能是机体应激反应的表现。

In order to veelfy the Subtype of IFN-α-1 structural gene in engineeing strain BMH_(71)-18/ pBV857 and to monitor any change of the gene that might happen after felmentation,we isolate its structural gene form harvestied culture via high density fermentation and then sequence it by the Sanger dideoxynucleotide chain termination method and by the Ray method based on adding separately synthesised primers.In eomparisoh of this Sequence with the sequece of original strain and the sequence reported in 1iterature,we...

In order to veelfy the Subtype of IFN-α-1 structural gene in engineeing strain BMH_(71)-18/ pBV857 and to monitor any change of the gene that might happen after felmentation,we isolate its structural gene form harvestied culture via high density fermentation and then sequence it by the Sanger dideoxynucleotide chain termination method and by the Ray method based on adding separately synthesised primers.In eomparisoh of this Sequence with the sequece of original strain and the sequence reported in 1iterature,we did not find any change of base pair.

为监测工程菌 BMH71-18/pBV867中所带干扰素结构基因的亚型,及在发酵培养中是否发生变化,我们将高密度发酵培养所得的菌体用 Sanger 双脱氧末端终止法及 Ray 的分步加入引物法,测定 IFN-α结构基因及其信号肽的碱基对序列。并把所测出的人α_1型干扰素基因的碱基对序列与原菌种及文献资料相核对,未发现有任何改变

 
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