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| | A yellow mutant of Aspergillus which can produce thermostable acid protease was obtained.Authors adjusted the fermentation conditions and come to a conclusion. The optimum medium is(g/L): bran 21 6, bean cake powder 48 4, NH 4Cl 2, KH 2PO 4 0 8, pH4~5 The enzyme activity can reach 6700 u/ml after 72 h when it is cultured at 30℃. | | 经筛选诱变得到一株产生耐温酸性蛋白酶的黑曲霉黄色变株 A-2 5 80 ,对其发酵条件进行了优化 :通过单因子试验和正交试验 ,确定其最适培养基为 (g/L) :麸皮 2 1 .6、豆饼粉 48.4、 NH4Cl 2、 KH2 PO40 .8,p H4~ 5 ,温度 3 0℃的条件下进行振荡培养 ,发酵 72 h,酶活可达 670 0 u/ml. | | 文摘来源 | |   |
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