In control group,the genotypes of H~+H~+ and H~+H~-showed higher apoCⅡ,apoCⅢ,HDL3b levels,but lower apoE/CⅢ,HDL2a,HDL2b levels compared with the genotype of H~-H~-(P<0.05).
FITC labeled HDL 3 were used to observed the cellular metabolic process of HDL 3. Results BSA (control group) mediated 2.9% cellular cholesterol efflux from the cells. In HDL 3 group and N-acetylimidazole-HDL 3 group,they were 40.7% and 8.7% respectively.
Results Compared with normal controls,the contents of small-sized preβ_1-HDL,HDL_(3a) were significantly higher(P<0.01),but those of large-sized HDL_(2a),HDL_(2b) were significantly lower (P<0.01).
In addition,males had significantly higher small-sized pre β_1-HDL,HDL_(3b)(P<0.05), but lower large-sized HDL_(2b)(P<0.01) than females in both normal controls and Ⅳ-HLP patients.
The incubation increased the content of lipid hydroperoxides in HDL3 significantly (by 32 and 40% calculated per ml of sample or mg of protein) and of malondialdehyde (by 27 and 34%, respectively) compared to control (incubation of HDL3 alone).
It seems likely that an increase in LPP (at least hydroperoxides) in HDL3 after their incubation with oxidized membranes occurs via transport of phospholipids containing LPP from erythrocyte membranes to lipoproteins.
The data on the ability of HDL3 to accept LPP from erythrocyte membranes in vitro suggest that HDL3 may have a protective action on cell membranes undergoing oxidation in vivo as well.
Im Bereich der HDL-Cholesterinfraktion reagierte vorzugsweise der Typ IIb mit einem signifikanten Anstieg, der sich im wesentlichen auf Ver?nderungen der HDL3-Subfraktion zurückführen lie?.
The HDL subfractions, however, seem to differ in their capacity to confer protection, with the large HDL2 subfraction appearing to be more important than the small HDL3 subfraction.
Compared to controls, at birth, macrosomic newborns had higher serum lipids, apolipoprotein A-I and B-100, and lipoprotein (very low density lipoprotein, low density lipoprotein, high density lipoprotein-2 and high density lipoprotein-3) levels.
This study examines the relationship between high density lipoprotein-3 (HDL-3) glycation and cholesteryl ester transfer mediated by cholesteryl ester transfer protein (CETP).
A method of using dextran-Sulfate ( D.S. ) and Catt in combination with poly-aerylamide gel preparative electrophoresis to isolate serum high density lipoprotein ( HDL ) in a pure form was developed. In this method HDL was precipitated by D.S, and Catt from serum freed previously of very low density lipoprotein (VLDL ) and low density lipoprotein ( LDL ) in a form of complex, which can be redissolved in 12% NaCl and reprecipitated by dilution and addition of D.S. and Catt. The above process was repeated twi...
An improved microanalytioal method for determination of serum high-density lipoprotein (HDL)-cholesterol is herein presented. 80 μl of serum in a small test tube was added to sodium phosphotungstate and Mg3+ mixed reagent. This solution was drawn up into a capillary tube, which was sealed and centrifuged at room temperature for 30 minutes for precipitation of both the serum low-density lipoprotein (LDL) and very low-density lipoprotein (VLDL). Cholesterol was then determined in the supernatant by means of i...
Serum high density lipoprotein (HDL)-cholesterol levels in 2,399 healthy subjects from newborn to 78 years of age were analyzed. Factors influencing on their variations were summarized as follows: (1) The frequency distribution of HDL-cholesterol levels was approximately normal in most age groups, although a skew to the right was observed before the age of 20 years for both sexes. (2) Serum HDL-cholesterol and ratio of HDL-cholesterol/total cholesterol varied with age. The mean value ( ± SD) of HDL-choleste...