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重组
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  recombinant
    STUDY ON THE RECOMBINANT DNA VACCINE COEXPRESSING NEWCASTLE DISEASE VIRUS F GENE AND CHICKEN IL-2
    新城疫病毒F基因与鸡IL-2重组DNA疫苗的研究
短句来源
    The Fusion Protein Gene of Newcastle Disease Virus Strain F_(48)E_8:Sequence Analysis and Expression by a Recombinant Fowlpox Virus
    新城疫病毒F48E8株融合蛋白基因和表达该基因的重组鸡痘病毒
短句来源
    PROTECTION OF CHICKENS AGAINST AVIAN INFLUENZA WITH RECOMBINANT FOWLPOX VIRUE EXPRESSING HA-NA, HA-NP OR NP OF AVIAN INFLUENZA VIRUS
    表达禽流感病毒HA-NA、HA-NP及NP基因重组禽痘病毒的构建及其免疫效力的研究
短句来源
    Construction of Two Recombinant Herpesviruses of Turkey rHVT-gB1/EGFP and rHVT-EGFP
    两种重组火鸡疱疹病毒rHVT-gB1/EGFP和rHVT-EGFP的构建
短句来源
    Recombinant Fowlpox Viruses Expressing HA from Subtype H9N2 of Avian Influenza Virus and/or Chicken Type Ⅱ Interferon and Their Protective Efficacies Against Homologous Challenge in Chickens
    表达和共表达H9亚型禽流感病毒血凝素基因和鸡Ⅱ型干扰素基因的重组鸡痘病毒
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  recombination
    Infectious Bursal Disease Virus's Influence on Certain Gene Expression of CEF Cells and Recombination of Infectious Laryngotracheitis Virus
    传染性法氏囊病病毒对CEF细胞某些基因表达的影响和传染性喉气管炎病毒的重组
短句来源
    Studies on the Cloning, Recombination and Expression of Calcium Metabolism Regulation Related Genes of the Chicken
    鸡钙代谢调节相关基因的克隆重组与表达研究
短句来源
    The S1 gene of Infectious bronchitis virus(IBV) was segmented and cloned into the expression vector pGEX-6p-1 by gene recombination technology. The glutathiones transferase ( GST) fusion proteins were induced by IPTG.
    利用基因重组技术将鸡传染性支气管炎病毒(IBV)S1基因分段(A和B段)克隆到pGEX-6p-1载体中,用IPTG诱导表达。
短句来源
    A recombinant plasmid comprising adenovirus genome with rabies virus glycoprotein gene in E1 region was constructed by homologous recombination.
    利用大肠杆菌内质粒间同源重组的方法,将狂犬病病毒G基因插入腺病毒基因组的E1基因区,构建了带有狂犬病病毒G基因的重组腺病毒质粒。
短句来源
    Western blot and IFA results showed that the ORF2 gene can express in the recombinant PRV.TCID_50 of this recombination virus on different cell lines was measured to be IBRS-2 10-7.1,CEF 10-4.8,Marc-145 10-6.8 TCID_50/0.1 mL,respectively.
    重组病毒在IBRS-2细胞上连续传15代后,遗传稳定。 该重组病毒在IBRS-2细胞上增殖滴度为10-7.1TCID50/0.1mL,在鸡胚成纤维细胞上增殖滴度为10-4.8TCID50/0.1mL,在Marc-145细胞上增殖滴度为10-6.8TCID50/0.1mL。
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  a recombinant
    The Fusion Protein Gene of Newcastle Disease Virus Strain F_(48)E_8:Sequence Analysis and Expression by a Recombinant Fowlpox Virus
    新城疫病毒F48E8株融合蛋白基因和表达该基因的重组鸡痘病毒
短句来源
    The foundational study on Canine Adenovirus type 2 as a recombinant vaccine vector
    犬2型腺病毒基因重组活疫苗的基础研究
短句来源
    Construction of a Recombinant Fowlpox Virus Expressing F gene of NDV and gB gene of ILTV and Immune Potency of the Recombinant Virus Against ILTV and NDV Challenge
    表达新城疫病毒F基因和传染性喉气管炎病毒gB基因重组禽痘病毒的构建及其免疫效力的研究
短句来源
    Construction of a Recombinant Pseudorabies Virus (PRV) Expressing HA Gene of Swine Influenza Virus (SIV) and Development of Recombinant Diagnostics for Detecting Antibodies Against PRV and SIV
    表达H3亚型猪流感病毒HA基因的重组伪狂犬病毒的构建及猪流感病毒和伪狂犬病毒特异性鉴别诊断方法的建立
短句来源
    Construction and Efficacy of a Recombinant Pseudorabies Virus Expressing GP5 of Porcine Reproductive and Respiratory Syndrome Virus
    表达PRRSV GP5蛋白的重组伪狂犬病毒的构建及其免疫效力的研究
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  “重组”译为未确定词的双语例句
    Isolation and Identication of PRV LA Strain and Construction of TK~- Recombinant PRV Expressing EGFP and CSFV-E2
    PRV鲁A株的分离鉴定及表达EGFP和CSFV-E2的TK~-重组伪狂犬病毒的构建
短句来源
    Preparation of Specific McAb-PE40 Chimeric Toxin and Its Kill Action to Cryptosporidium Parvum
    特异性单抗-PE40重组毒素的制备及其对隐孢子虫杀灭作用研究
短句来源
    Characterization of Caged Pet Birds H3N8 Influenza a Virus and Generation of a Reassortant Virus by Reverse Genetics
    宠物鸟H3N8亚型流感病毒的鉴定及利用反义遗传学技术产生重组病毒
短句来源
    Mechanisms of in Vitro Delivering CD8~ +T Epitopes Through Attenuated Bacterica and Immunobiological Properties of Attenuated Salmonella Typhimurium Harbouring DNA Vaccine Against Newcastle Disease Virus
    重组减毒细菌运送CD8~+T细胞表位的机理及携带新城疫病毒DNA 疫苗鼠伤寒沙门氏菌的免疫生物学特性研究
短句来源
    Genomic Characterization of an Avian Influenza Virus of H9N2 Subtype and Generation of H9N2 Reassortants and Attenuated H5 Recombinants by Reverse Genetics
    H9N2亚型AIV基因组序列分析及用反向遗传技术产生多个H9N2和H5重组流感病毒
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  recombinant
Screening of Peptide Inhibitors of TACE from a Phage Display Random 15-Peptide Library by Recombinant TACE Ectodomain
      
After Ni2+-NTA resin affinity chromatography, the purity of the recombinant T800/T1300 protein was more than 90%.
      
The recombinant plasmid containing mutant E83V pPIC3.5K-lip-E83V was expressed in Pichia pastoris GS115.
      
A recombinant strain of Salmonella choleraesuis C500, containing a eukaryotic expression plasmid pBO1 with the immune-dominant epitope of foot-and-mouth disease virus, was constructed.
      
Specific immune response to this recombinant strain was evaluated by oral administration of the recombinant live bacteria pBO1/S.
      
更多          
  recombination
Base composition in the sequences was high in A+T content between 64.17% and 64.95%, and no recombination event occurred (Rm = 0).
      
Ex situ conservation efforts should focus on enhancing the exchange of seeds and seedlings among populations to facilitate gene exchange and recombination, and to help conserve genetic diversity.
      
The ability for heterophilic antigen-binding mode of interaction was apparently acquired due to the introduction of recombination-activating retroviral genes (RAG1 and RAG2) into the genome of Gnathostomata ancestors.
      
This band is attributed to the charge recombination between acceptor QA- and a redoxactive histidine residue on the donor side of PS II.
      
FEN-1 participates in DNA replication, repair, and recombination.
      
更多          
  a recombinant
A recombinant strain of Salmonella choleraesuis C500, containing a eukaryotic expression plasmid pBO1 with the immune-dominant epitope of foot-and-mouth disease virus, was constructed.
      
Cloning and expressing a recombinant human tissue inhibitor of metalloproteinase-2 (TIMP-2) in methylotrophic yeast Pichia pasto
      
The verified gene of TIMP-2 was cloned to the Escherichia coli-yeast shuttle vector of pPIC9 to construct a recombinant plasmid pPIC9-T2.
      
Construction of a recombinant yeast strain converting xylose and glucose to ethanol
      
fluorescens strain 5RL, harboring a recombinant construct in the chromosome, were more resistant to the combined action of the stress factors under study than P.
      
更多          


Experiments of cloning and expressing sequences coding for a nonstructural protein gene NSI from the long leftward open reading frame of the autonomous parvovirus FPV by using bacteriophage λgtll were conducted.These experiments composed of preparing the target gene DNA, extracting the λgtll DNA, Iigating the target gene DNA with λgtll DNA, in vitro packaging, screening the phage plagues and lysogens, detecting the recombinant gene products.Fusion proteins expressel by the recombinant gene were thus obtaincd.These...

Experiments of cloning and expressing sequences coding for a nonstructural protein gene NSI from the long leftward open reading frame of the autonomous parvovirus FPV by using bacteriophage λgtll were conducted.These experiments composed of preparing the target gene DNA, extracting the λgtll DNA, Iigating the target gene DNA with λgtll DNA, in vitro packaging, screening the phage plagues and lysogens, detecting the recombinant gene products.Fusion proteins expressel by the recombinant gene were thus obtaincd.These fusion proteins were specifically bound by both antisera raised against one of the fusion proteins and antisera from a canine parvovirus(CPV) in fected dog.The results obtained showed that the bacteriophage λgtll is a good vector for gene manipulation and its extending use will open vast vistas in serodiagnosis and vaccine prevention.

大肠杆菌噬菌体λgt11是在原有基础上经不断改进而组建成的一个新的基因载体,通过一系列检测方法的建立,已成为一个完整的基因操作系统。本文应用此系统对细小病毒的非结构蛋白基因NSI进行了克隆和表达试验,包括目的基因的制备、载体DNA的提取、基因拼接、噬菌体的体外包装、重组噬菌体蚀斑和溶原菌的抗体探子筛选以及重组体基因产物的检测,最后获得了由重组体基因表达产生的融合蛋白质。此蛋白质能被兔源抗NSI抗体和自然感染细小病毒的犬血清特()性地结合。试验结果表明,λgt11是基因操作的一个有效的载体系统,它在疫病的血清学诊断和疫病预防的研究上具有广阔的应用前景。

Bam HI partial digestion fragments from Pseudomonas mallei chromosome were ligated into the Bam HI site of the cloning vector pBR 322 and tranferred into E. coli strain RRI. In about 5000 tran(?)rmants there were 182 transformants which have AP' TC' phenotype. The morphological and biochemical properties of these transformants were the same as the original host bacterium. The No.50 and the No.28 transformants which expressed the specific antigen of Pseudomonas mallei were screened by radioautography and ELISA....

Bam HI partial digestion fragments from Pseudomonas mallei chromosome were ligated into the Bam HI site of the cloning vector pBR 322 and tranferred into E. coli strain RRI. In about 5000 tran(?)rmants there were 182 transformants which have AP' TC' phenotype. The morphological and biochemical properties of these transformants were the same as the original host bacterium. The No.50 and the No.28 transformants which expressed the specific antigen of Pseudomonas mallei were screened by radioautography and ELISA. The No.50 transformant is a high expression strain while the No.28 is a weaker one. The positive expression of foreign gene in the No.50 transformant was observed with immuno-electron microscopy. The expression can be seen at the outer membrane and inner Part of the cell. The No.50 transformant possessed some expected antigenicity. Immunizing guinia pigs with this strain, it may induce production of antibody against Ps. mallei. The characters of recombinated plasmid pBM50 of the No.50 transformant were determined.It was found that there are no Hind Ⅲ, Pvu Ⅱ, SalⅠ sites but an Eco RI site at the inserted fragment of the plasmid. This plasmid is about 10kb. The inserted foreign gene is about 5.7kb. The plasmid can be cut into two parts with Eco RI. The size of the two parts were 3.9 and 6.1 kb respectively.

用“鸟枪法”分子克隆技术将鼻疽杆菌基因重组到载体质粒pBR 322的BamHⅠ酶切位点上,再转化到大肠杆菌RRⅠ中。经过耐药表型筛选,在5000个转化菌中找出了182个具有重组质粒的细菌株,其一般细菌学性质与大肠杆菌RRⅠ相同。用放射自显影法及ELISA法筛选出50号阳性表达菌株及28号弱阳性表达菌株。用免疫电镜法观测50号转化菌,可见在细菌外膜及内部都有外源基因表达。50号转化菌只与抗鼻疽菌血清反应,不与抗类鼻疽菌血清反应;它具有较弱的免疫原性,给豚鼠注射后,可使豚鼠产生抗鼻疽杆菌抗体;在其重组质粒pBM 50的外源基因插入片段上无HindⅢ、PvuⅡ及SalⅠ酶切点,只有一EcoRⅠ酶切点。质粒pBM 50的分子量约为10 kb(kilobase pair),插入的外源基因片段为5.7 kb。Eco RⅠ可把pBM 50质粒切成分子量分别为3.9及6.1 kb两部分,从而推断出质粒pBM 50的简单酶切图。

This paper gives a brief account of advances during the last decade ontechnology related to animal husbandry.These include realms in(1)animalhygiene such as new dianostic,pharmaceutical and immunological techniques;(2)feed supply such as single cell protein,amino acid fermentation and cel-lulotic disintegration;(3)superovulation and embryo transfer;(4)genetic en-gineering such as cloning,gene transfer and fusion,(5)physiological manipu-lation such as control of metabolic rate and protein synthesis,promotiongrowth...

This paper gives a brief account of advances during the last decade ontechnology related to animal husbandry.These include realms in(1)animalhygiene such as new dianostic,pharmaceutical and immunological techniques;(2)feed supply such as single cell protein,amino acid fermentation and cel-lulotic disintegration;(3)superovulation and embryo transfer;(4)genetic en-gineering such as cloning,gene transfer and fusion,(5)physiological manipu-lation such as control of metabolic rate and protein synthesis,promotiongrowth and milk secretion.Some had already been put to use and others areawaiting application in the near future.

本文扼要介绍了近十年生物技术在家畜保健、饲料生产、胚胎培育、基因工程和生理调节领域的进展。兽医方面,有诊断新法,生物防治与抗病免疫;饲料方面有细胞蛋白与氨基酸的生产工艺、纤维的微生物分解;胚胎方面,有超数排卵,合子分割与移植;道传工程有单克隆分离培养、基因重组与融合;生理调节有节约维持消耗、生长的促进与控制、泌乳的增产等。有些项目已用于生产,有的尚在开拓中。

 
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