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癌组织
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  cancer tissues
    USING PCR-SSCP METHOD TO DETECT THE ABNORMALITY OF HPV16 E_7 GENE IN THE CERVICAL CANCER TISSUES
    用PCR-SSCP方法研究子宫颈癌组织中人乳头瘤病毒E_7基因的变异
短句来源
    Positive cytoplasmic LN appeared in 63.3% of the cancer tissues.
    60.3%癌组织中癌细胞胞浆层粘连蛋白阳性。
短句来源
    Electrophoretic patterns of the AP-PCR products from stomach and its cancer tissues are of polymorphic characteristics
    人胃及其癌组织的RAPD多态性分析
短句来源
    Further more, in the EBV-positive cancer tissues, EBV genome has been detected in almost all malignant cells. These finding suggest that EBV may be the ideal target to cure the EBV-positive tumor. So we can take measure to kill EBV-positive tumor cells specifically based on the difference between EBV-positive tumor cells and normal cells.
    研究表明在EBV相关肿瘤组织中,EBV只感染癌细胞而不感染正常细胞,而EBV阳性癌组织中,EBV基因组存在于几乎所有的癌细胞中,提示EBV可能是治疗EBV阳性肿瘤的理想靶点,因此可以利用EBV阳性肿瘤细胞与正常细胞的差异,采用适当方法选择性地杀伤EBV阳性的肿瘤细胞。
短句来源
    In order to knowbetter about the abnormality of E_7 gene in the cervical cancer tissues, a sensitive PCR-SSCP methodwas used to detect 33 specimens. Twenty-sevenspecimens had mutations in their single strandDNA, which revealed that the mutations of E_7 genewere the frequent phenomina in the cervical cancers.
    进一步用灵敏的单链构象多态性分析法(SSCP)对33例阳性标本进行分析,发现有27例单链DNA迁移率与正常对照有不同,推测均发生了DNA序列的改变,提示HPV-16E7基因的变异在子宫颈癌组织中是较常见的现象,其与子宫颈癌的发生有一定的关系。
短句来源
  cancer tissue
    Western blot revealed that McAbGB2 recognized antigen with molecular weight of 116 and 45 ku. The antigen recognized by McAbGB2 exists in breast cancer tissue and serum.
    McAbGB2抗原决定簇不存在于铁蛋白及癌胚抗原; 蛋白质印边检测表明McAbGB2抗原有两条区带,分子量分别为116及45ku,分布于血液及癌组织
短句来源
    Both the epitope of antigen in feces and cancer tissue are protein in nature but with different molecular weight.
    粪便中也存有CL-3对应的肿瘤相关抗原,其性质同癌组织的相关抗原,但分子量明显小于它。
短句来源
    Results: With leisions progression,the number of positive cell per mm2 by PCNA increased,there was a significant difference (P<0.05) each other in all other groups except that normal mucosa one did not valublely correlated with superficial gastritis. In the gastric cancer tissue,expression of PCNA correlated positively with biological behavior of gastric cancer.
    结果:PCNA免疫阳性细胞数随病变发展呈上升趋势,其定量分析数据除浅表性胃炎与正常胃粘膜无明显差异外,其余各组间差异显著(P<005),癌组织中,PCNA表达变化与胃癌生物学行为密切相关。
短句来源
    but significant correlations was not observed between the ratio and clinical features Conclusion: Expression of CD44 epithelial form is obviously higher in colorectal cancer tissue than in matched normal mucosa The E/H ratio is not associated with progression and metastasis in colorectal cancer The role of CD44 variant forms in progression and metastasis of colorectal cancer needs to be elucidated
    结果 :E/H值癌组织明显高于相应正常粘膜组织 ,但未发现与肿瘤转移、浸润深度等临床资料有关。 结论 :CD44上皮源性亚型mRNA在大肠癌组织中表达明显增高 ,E/H值与大肠肿瘤进展转移无明显相关性。
短句来源
  “癌组织”译为未确定词的双语例句
    he technique of add-on PCR was used for isolating HPV16 E7 gene from cervical carcinomabiopsy DNA in Hubei province.
    采用加端聚合酶链反应技术,从湖北地区一宫颈癌患者癌组织DNA中分离出人乳头瘤病毒16型(HPV16)E7基因,并在pUC18载体中克隆。
短句来源
    The results showed that AF 091521, AF 091520, AF 152605 and AF 091517 cDNA sequences had distinct expression difference between primary cultural normal nasopharyngeal epithelial cell and NPC biopsies, and AF 091521, AF 091517 genes all had two transcripts whose sizes were 1.5, 2.3 and 1.1, 1.4 kb respectively, while AF 091520 and AF 152605 gene expressed one trans cript only, respectively, whose sizes were 1.6 and 2.2 kb.
    结果显示 ,AF0 915 2 1、AF0 915 2 0、AF15 2 6 0 5、AF0 915 17等cDNA序列在正常鼻咽上皮与鼻咽癌组织中存在表达差异 ; AF0 915 2 1和AF0 915 17基因均有 2个转录本 ,大小分别为 1.5、2 .3和1.1、1.4kb ;
短句来源
    The homogenate of pericarcinous tissues contained a higher levels of NO than those of carcinous tissues (P< 0.05) and it was more obvious when the muscularis was invaded (P< 0.001).
    癌周组织匀浆NO含量高于癌组织NO含量(P<0.05) ,在癌侵及肌层时更为明显(P<0.001) ;
短句来源
    After amplified with PCR, HPV16-E7 gene was cloned into pMD18-T vector, then the identified HPV16-E7 gene was cloned into shuttle plasmid pGAPZαA. The recombinant plasmid were linearized and transformed into pichia pastoris. Before fermentation, the positive clone was screened with zeocin +.
    依据新疆维吾尔妇女宫颈癌组织克隆获得的HPV16-E7基因序列设计引物,并分别在5’引物和3’引物中引入了EcoR Ⅰ和Xba Ⅰ酶切位点,经PCR扩增后连接到pMD18-T载体上,再将HPV16-E7克隆至穿梭质粒pGAPZαA上,获得的重组穿梭质粒pGAPZαA-E7经线性化后.
短句来源
    Methods The local HPV16-L1 gene was generated by polymerase chain reaction, inserted into pGEM-T Easy cloning vector, and then subcloned into the eukaryotic expression vector pcDNA3.1(-). The expression of recombinant plasmid pcDNA-L1, transferred in Cos-7 cells, was identified by immunofluorescence.
    方法采用PCR技术从宫颈癌组织中获得L1基因,以pGEM-T Easy为克隆载体构建重组质粒 pGEM-T-L1,进行限制性核酸内切酶及核苷酸序列分析,再将L1基因亚克隆到真核表达载体 pcDNA3.1(-)中,构建地方株HPV16L1基因疫苗,转染COS-7细胞,通过IFA试验验证L1蛋白的表达。
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  cancer tissues
To determine the correlation of Sef with human diseases, Sef expression patterns were observed in cell lines and human cancer tissues.
      
Results demonstrated that Sef might be up-regulated in the cancer tissues suggesting a possible role of Sef in pathophysiology of human diseases.
      
LOH and MSI of the FHIT were detected by using PCR at 4 microsatellite loci: D3S 1300, D3S 4103, D3S 1481, D3S 1234 in cancer tissues from 50 patients with primary GC, with normal mucosa acting as matched controls.
      
Our results showed over expression of EMMPRIN in advanced stages of breast cancer tissues compared with benign tumor tissue samples.
      
The expression level of Pescadillo was increased significantly in breast cancer tissues compared with their paired margin tissues.
      
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  cancer tissue
In addition, associated with the repair of cancer tissue (or relapse) after chemotherapy, telomerase activity in stem cells was markedly increased.
      
In a previous study, the content of sex hormone receptors in breast cancer tissue was found to be significantly correlated with tumor laterality and the time of year of surgery.
      
Influence of zinc, managenese and selenium on superoxide dismutase activity in lung cancer tissue and cell in culture
      
Objective: To investigate the expression of novel multidrug resistance transporter (BCRP gene) from human MCF-7/AdrVp breast cancer cells in normal lung tissue and non-small lung cancer tissue.
      
Conclusion: The expression of LEA is related to the differentiation degree of colorectal cancer tissue.
      
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An esophageal cancer epithelial cancer cell line (EC-56) derived from a surgically excised specimen of an esophageal squamous cell carcinoma in a 35 year-old male patient was established in vitro. Two clonal lines, designated EC-56 C-2 and C-5, were isolated from EC-56 and characterized. The C-2 line consisted of fusiform cells, while C-5 of epithelioid cells. Both cell lines had properties usually found in malignant cell lines, such as, the ahility to grow in vitro unlimitedly, aneu-ploid karyotypes, microvilli...

An esophageal cancer epithelial cancer cell line (EC-56) derived from a surgically excised specimen of an esophageal squamous cell carcinoma in a 35 year-old male patient was established in vitro. Two clonal lines, designated EC-56 C-2 and C-5, were isolated from EC-56 and characterized. The C-2 line consisted of fusiform cells, while C-5 of epithelioid cells. Both cell lines had properties usually found in malignant cell lines, such as, the ahility to grow in vitro unlimitedly, aneu-ploid karyotypes, microvilli formation, as well as agglutination by coneanavalin A. The C-5 line could form colonies in semisolid agar medium and induce tumors in ATS-treated suckling mice, while C-2 line failed to do so under similar conditions.Desmosomes on the cell membranes of both clonal lines were observed with an electron microscope, thus providing a further evidence concerning the epithelial nature of these cell lines.It is suggested that the results herein reported may reflect some differences in malignancy among individual clonal lines. Should this be true, it may provide a useful tool for studying carcinogenesis and decarcinogenesis in vitro.

从一名35岁男性食管鳞癌病人的手术切除的癌组织标本,经体外培养建立了一个食管癌上皮细胞系(EC-56),并由此分离出两个细胞克隆系,分别命名为EC-56 C-2及C-5,进行了一系列鉴定。C-2系由梭状细胞组成,而C-5系则由类上皮细胞组成。这两个细胞克隆系都具有恶性细胞系的一般特性:例如能在体外长期连续传代;具有非整倍体核型;有丰富的微绒毛以及能被刀豆球蛋白A凝集等。C-2和C-5系除形态不同外,在生物学特性上也有差别。C-5系能在半固体琼脂培养基中形成集落,并能在以抗胸腺免疫血清处理的乳小鼠体内形成肿瘤,而C-2系列缺乏上述两种能力。 在电镜下,这两个细胞克隆系的细胞膜上均可发现桥粒,进一步表明它们具有上皮细胞的特征。本实验结果表明,从人食管癌细胞系中可分离到恶性程度不同的克隆系,而且有可能把它们用于肿瘤细胞的痛变和去恶化研究。

Specimens of gastric mucosa were obtained from 16 cases of fetal stomach, aging from 11 weeks to full term, 5 cases from gastric adenocarcinoma, one case from gastritis and one case from normal adult. The contents of their mucosubstances were compared histochemically with Mowry's alcian blue and PAS techniques. The results are as follows:The mucosubstances of gastric mucosa undergo a conspicions change during the fetal life. In general, acid mucopolysaccharide appear at first in the epithelium and rudimentary...

Specimens of gastric mucosa were obtained from 16 cases of fetal stomach, aging from 11 weeks to full term, 5 cases from gastric adenocarcinoma, one case from gastritis and one case from normal adult. The contents of their mucosubstances were compared histochemically with Mowry's alcian blue and PAS techniques. The results are as follows:The mucosubstances of gastric mucosa undergo a conspicions change during the fetal life. In general, acid mucopolysaccharide appear at first in the epithelium and rudimentary gastric pits and it is replaced subsequently by neutral mucin. This mucosaccharide replacement varies within different areas and stages. The neutral mucin always takes its appearance about the 11th week at the cardiac and pyloric regions where the mucosubstance have begun to reveal pruple colour in AB-PAS reaction. After the 4th month, the neutral mucosacchaide has completed its replacement in both areas except the lower parts of pyloric glands which still show some mixed mucins. In the corpus tomach, acid mucin is present almost during the whole fetal life but converts into mixed type only at birth. The parietal cell do not contain any mucous material during its histogenesis, but the chief cell may contain some alcianophilic granules which will change into faintly PAS-positive at birth. With alcian blue at low pH, it proves that most of the acid mucosaccarides are sulfated mucosubstance.Adjacent to the bottoms of gastric pits and the necks of gastric glands, some acid mucin often remains in 1he epithelial and neck mucous cells of normal adult just as those of newborn. The acid mucosubstance occurs in various degree within the spaces or lumen of adenocarcinoma tissue. It could be considered that the bottoms of pits and necks of glands would be a cellular bases for further development of gastric carcinnoma under certain conditions.

1.胎儿胃上皮内粘液物质随组织不断分化而由酸性向中性转变。足月时除贲门部与幽门部转变成中性粘液外,胃体部上皮仍未完全变成中性粘液,小凹底部与胃腺颈部仍保留有较多的酸性粘液。 2.成人胃体部之小凹底部与腺颈部仍为保留少量酸性粘液的唯一部位,从粘液分泌功能看,可能是整个胃粘膜内分化较低之区域。 3.正常成人幽门部之肠化生区与癌组织附近的肠化生区出现含有酸性粘液之上皮,以及腺癌组织含有不同数量的酸性粘液提示,它们可能来源于小凹底部与腺颈部之低分化细胞,表明有向胚胎性胃组织逆转的倾向。

Sulfonated aluminum phthalocyanine (ALSPC), a new photosensitizer of pontential use in photodynamic cancer therapy, the intermediates of its photo-sensitization were studied in photochmical level. Using 2 ,2,6,6,-tetramethy 1-1 -piperidone (TMHP) as a probe, the nitroxyl free radical produced in ALSPC photochemical reaction was detected by ESR spectrum technic. The effects of enhancement and inhibition of D2O, NaN3 β-carotene, cytochrome C, SOD, mannitol and alcohol be tested, and the results shown that there...

Sulfonated aluminum phthalocyanine (ALSPC), a new photosensitizer of pontential use in photodynamic cancer therapy, the intermediates of its photo-sensitization were studied in photochmical level. Using 2 ,2,6,6,-tetramethy 1-1 -piperidone (TMHP) as a probe, the nitroxyl free radical produced in ALSPC photochemical reaction was detected by ESR spectrum technic. The effects of enhancement and inhibition of D2O, NaN3 β-carotene, cytochrome C, SOD, mannitol and alcohol be tested, and the results shown that there were 102 Of and OH intermediates produced in ALSPC photochemical reaction but the O seemed the main product in process.

铝酞菁(AISPC)是有潜力用于光动力癌症疗法(PDT)的新光敏剂.本文应用ESR波谱技术,对AISPC光敏反应的中间产物进行实验研究,探讨了重水,β—胡萝卜素、迭氧钠、SOD、细胞色素C、甘露醇及乙醇等活性氧增进或抑制剂对光敏反应的影响.结果表明,(Al-SPC)在水溶液中的光敏反应,除之有~1O_2生成外,还有O_2~-及·OH自由基产生.但以~1O_2.中间产物为主.光敏疗法是一种有希望的肿瘤治疗新方法.但由于光敏剂血卟啉衍生物(HPD)组份复杂、红光吸收太弱等局限性,1985年以来,探寻结构明了、成份稳定、主吸收峰位于红光区(人体绍织透射的最佳波段)的新光敏剂的研究工作不断增多,其中尤以对酞菁类染料的研究较为集中.我组在研究出可溶于水的磺化铝酞菁后,亦已在离体人癌细胞及小鼠移植瘤实验中(待发表)获得杀伤癌细胞、癌组织的功效.随着酞菁染料作为新光敏剂的潜力被挖掘,其反应机制的研究也受到重视,但目前尚不甚明了,且较系统的研究也不多.本文应用ESR波谐技术,可2、2.6、6—四甲基—4羟基哌啶醇为探针,并利用~1O_2,0_2~1·OH消除剂,研究了磺化铝酞菁的光敏反应中间产物,探讨了其可能的反...

铝酞菁(AISPC)是有潜力用于光动力癌症疗法(PDT)的新光敏剂.本文应用ESR波谱技术,对AISPC光敏反应的中间产物进行实验研究,探讨了重水,β—胡萝卜素、迭氧钠、SOD、细胞色素C、甘露醇及乙醇等活性氧增进或抑制剂对光敏反应的影响.结果表明,(Al-SPC)在水溶液中的光敏反应,除之有~1O_2生成外,还有O_2~-及·OH自由基产生.但以~1O_2.中间产物为主.光敏疗法是一种有希望的肿瘤治疗新方法.但由于光敏剂血卟啉衍生物(HPD)组份复杂、红光吸收太弱等局限性,1985年以来,探寻结构明了、成份稳定、主吸收峰位于红光区(人体绍织透射的最佳波段)的新光敏剂的研究工作不断增多,其中尤以对酞菁类染料的研究较为集中.我组在研究出可溶于水的磺化铝酞菁后,亦已在离体人癌细胞及小鼠移植瘤实验中(待发表)获得杀伤癌细胞、癌组织的功效.随着酞菁染料作为新光敏剂的潜力被挖掘,其反应机制的研究也受到重视,但目前尚不甚明了,且较系统的研究也不多.本文应用ESR波谐技术,可2、2.6、6—四甲基—4羟基哌啶醇为探针,并利用~1O_2,0_2~1·OH消除剂,研究了磺化铝酞菁的光敏反应中间产物,探讨了其可能的反应机制.

 
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