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癌组织
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  cancer tissue
    Methods PCR was used to amplify HPV16E4 gene from cervical cancer tissue infected by HPV16.The plasmid,pET21b-HPV16E4,was then constructed by inserting the amplified HPV16 E4 gene into pET21b,a prokaryotic expression vector. PCR was used to screen the positive recombinant clones.
    方法从宫颈癌组织中提取DNA,用PCR方法扩增HPV16E4 DNA片段,定向克隆到原核表达载体pET21b质粒中,利用菌液PCR方法筛选重组阳性菌株。
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    Zinc,manganese aud selenium were respectively added to the medium in which the Lewis lung cancer tissue and A_(549) lung cancer cell line were cultur- ed for 24 hours and then the superoxide dismutase(SOD)activity of the tiss- ue and the cell was measured in every group.
    在培养的 Lewis 肺癌组织块和 A_(549)肺癌细胞系细胞培养液中加入微量元素锌、锰和硒,24小后测定各组的 SOD 活性发现:锌、锰明显地提高了癌组织和癌细胞的 SOD 活性.
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    Immunotherapy of Cancer by Tumor Vaccine Prepared from Paraffin Embeded Cancer Tissue (PEIV) in 615 Mice
    石蜡包埋癌组织制备的瘤苗(PETV)对荷瘤615小鼠的治疗作用
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    Results: With leisions progression,the number of positive cell per mm2 by PCNA increased,there was a significant difference (P<0.05) each other in all other groups except that normal mucosa one did not valublely correlated with superficial gastritis. In the gastric cancer tissue,expression of PCNA correlated positively with biological behavior of gastric cancer.
    结果:PCNA免疫阳性细胞数随病变发展呈上升趋势,其定量分析数据除浅表性胃炎与正常胃粘膜无明显差异外,其余各组间差异显著(P<005),癌组织中,PCNA表达变化与胃癌生物学行为密切相关。
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  “癌组织”译为未确定词的双语例句
    Restriction analysis and sequencing were used to confirm the construction of pET21b-HPV16E4.Results HPV16E4 gene(0.3kb) was amplified and recombinated into pET21b vector.
    提取重组质粒,利用酶切图谱分析方法和核酸序列测定验证重组质粒pET21b-HPV16E4构建的正确性。 结果用PCR方法从宫颈癌组织中扩增出HPV16 E4片段,大小为0.3kb。
短句来源
    LMP1 is the only oncogene derives from
    从鼻咽癌组织中获得的EBV BNLF1基因(N-LMP1,N-LMP1)与标准的B95-8细胞来源的BNLF1基因(B-LMP1)序列存在一定的差异。
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    The modified two-protocol PCR technique was used for detection of the HPV-16 E_6/E_7-related sequences in 34 Chinese cervical cancers,the positive percentage of HPV-16 E_6/E_7 in cervical cancers, was 67.6%(23/34).
    应用改良的二步温控法 PCR 技术,检测34例人宫颈癌组织 DNA,发现宫颈癌中 HPV-16 E_6/E_7相关序列存在的阳性率为67.6%(23/34)。
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    The results showed that P ̄(21) protein expressed in 27 cases of scrotum and 8 cases of paracarcinoma tissues,but they did not express in 20 cases of normal scrotum. Expression of P ̄(21) protein was related to the pathological grading and clinical stage.
    结果27例癌组织,8例癌旁组织中有P~(21)蛋白表达,而20例正常阴囊组织中P~(21)阴性。
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    Methods Immunohistochemistry (LAB SA and S P) was used to detect bFGF、FGFR I and Ki67 antigen.
    方法 应用免疫组化S P法和Lab SA法检测bFGF、FGFR I、Ki67抗原在肝胆管结石症及结石合并肝胆管癌组织中的表达。
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  cancer tissue
In addition, associated with the repair of cancer tissue (or relapse) after chemotherapy, telomerase activity in stem cells was markedly increased.
      
In a previous study, the content of sex hormone receptors in breast cancer tissue was found to be significantly correlated with tumor laterality and the time of year of surgery.
      
Influence of zinc, managenese and selenium on superoxide dismutase activity in lung cancer tissue and cell in culture
      
Objective: To investigate the expression of novel multidrug resistance transporter (BCRP gene) from human MCF-7/AdrVp breast cancer cells in normal lung tissue and non-small lung cancer tissue.
      
Conclusion: The expression of LEA is related to the differentiation degree of colorectal cancer tissue.
      
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The purpose of the present experiment was to observe the morphological characteristics and invasiveness of a transplantable cancer NO 27 (U 27) after serial passages about 20 years. The results were as follows: The morphological characteristics of this tumor strain was low-differentiated carcinoma which were demonstrated by light microscopy and transmission electron microscopy. In the cytoplasm the single irregular distribution of the tensile fibrils and some semi-desmosome between adjacent cells were discovered...

The purpose of the present experiment was to observe the morphological characteristics and invasiveness of a transplantable cancer NO 27 (U 27) after serial passages about 20 years. The results were as follows: The morphological characteristics of this tumor strain was low-differentiated carcinoma which were demonstrated by light microscopy and transmission electron microscopy. In the cytoplasm the single irregular distribution of the tensile fibrils and some semi-desmosome between adjacent cells were discovered by transmission electron microscopy. Two types of cancer cells were observed on the surface of the cancer nest by scanning electron microcopy:Ⅰ), There were many microvilli on the surface of the first type cancer cells; 2). The surface of the cancer cells of the second type were more flat sometimes with single small blebs. Many cancer cells had been released from cancer cell clump after 3 days of intramuscular implantation. Later they actively invaded into the spaces between muscle fibers in the form of amebae. Some cancer cells stretched out their filopodia directly in contact with target cells. The degenerative and atrophic changes of muscle tissue appeared in the invasive area of cancer cells. Finally these changes were leading to the disappearance of the entire structure of the muscle with the replacement by cancer tissues.

本文目的是检查可移植性子宫颈癌(U27),经二十多年传代后形态的改变特征及其侵袭能力。组织学和透射电镜检查证明本瘤株已变为低分化的上皮癌;电镜检查胞浆内仅查见少数张力原纤维,细胞连接仅见半桥粒及类桥样结构,未见典型鳞状上皮癌的特征。扫描电镜检查可见癌细胞表面有两种主要表现类型:一种带有大量微绒毛;另一种表面比较光滑仅有少量泡状突起。肌肉内小块移植后3天,即见大量癌细胞脱离癌实体,向肌肉纤维之间活跃侵袭,侵袭方式主要沿肌细胞之间的自然间隙连续侵入,也见沿肌细胞表面呈阿米巴样运动,有的癌细胞表面伸出不同突起与靶细胞直接连接,有的癌细胞伸出丝状伪足,用其分叉的末端紧紧抓住靶细胞表面。在癌组织侵袭之处可见肌细胞变性、进而萎缩,最终可完全被癌细胞所代替。

The surface features of human squamous cell esophageal carcinoma were observed by applying scanning electron microscope. The observation results are as follows: 1. The surface features of carcinoma tissue vary with the macroscopic types of esophageal carcinoma. 2. The surface features of carcinoma cells: Four different structures, i. e. fragment material, cell coat, plasmolemma, and cytoskeleton were found in turn from the most superficial surface to deep ulcerated area. 3. The junctions of carcinoma cells:...

The surface features of human squamous cell esophageal carcinoma were observed by applying scanning electron microscope. The observation results are as follows: 1. The surface features of carcinoma tissue vary with the macroscopic types of esophageal carcinoma. 2. The surface features of carcinoma cells: Four different structures, i. e. fragment material, cell coat, plasmolemma, and cytoskeleton were found in turn from the most superficial surface to deep ulcerated area. 3. The junctions of carcinoma cells: The junctions between small spherical carcinoma cells and the junctions between carcinoma cells covered with cell coat were not well connected. The junctions between well-differentiated polygonal carcinoma cells were connected with the cytoplasmic processes.

利用扫描电镜观察人食管鳞癌的表面形态。观察结果如下: 1.不同肉眼类型的食管癌,其癌组织表面形态各异。 2.癌细胞表面形态:从细胞最表面直到细胞溃烂处,依次可见4层不同结构,即碎屑物质、细咆外衣、质膜及细胞骨架。 3.癌细胞的细胞连接:小圆球形及包裹细胞外衣的癌细胞,其连接不好。分化较好的多角形癌细胞以胞浆突起相连接。

Nucleic acid molecular hybridization ofthe DNA of human nasopharyngeal tissuesfrom 20 cases was studied.14 of themwere nasopharyngeal carcinoma(NPC), 2cases malignant lymphoma,2 cases naso-pharyngitis,1 case papilloma and 1 casenasopharynx in a fetus.These DNA weredigested with the restriction enzyme BamHI in standard conditions,then the frag-ments were separated by electrophoresis on0.8% agarose gels and transferred to nitrocel-lulose filters by Southern blot method,and were hybridized with nick translated~(32)P-labelled...

Nucleic acid molecular hybridization ofthe DNA of human nasopharyngeal tissuesfrom 20 cases was studied.14 of themwere nasopharyngeal carcinoma(NPC), 2cases malignant lymphoma,2 cases naso-pharyngitis,1 case papilloma and 1 casenasopharynx in a fetus.These DNA weredigested with the restriction enzyme BamHI in standard conditions,then the frag-ments were separated by electrophoresis on0.8% agarose gels and transferred to nitrocel-lulose filters by Southern blot method,and were hybridized with nick translated~(32)P-labelled EBV-W(Bam HI) probe.The autoradiograme showed that the naso-pharyngeal carcinoma had similarpattern of restriction enzyme and thatthe presence of 6.7. 5.3, 3.1 and 2.0Kb of four hybridizing bands wasobserved in the DNA of NPC. Thesepositive hybridizing bands had a geneticsequence homologous to EBV-W (BamHI) fragment.Hybridizing bands werenot observed in the DNA of non-cancernasopharyngeal tissue.The experimentalresults demonstrated the presence of EB-viral genomes in nasopharyngeal carcinomatissue. The close relationship betweenEB-virus and the carcinogenesis ofnasopharyngeal carcinoma was furtherevidenced by molecular hybridizationtechniques.This study has provided someexperimental evidence for the elucidationof the mechanism of carcinogenesis andpathogeny of nasopharyngeal carcinoma.

20例人鼻咽部组织DNA,经酶切后,与EBV-W(Bam H Ⅰ)片段探针作核酸分子杂交。Southern印迹杂交发现鼻咽低分化癌组织DNA,经Bam H Ⅰ酶切后出现6.7、5.3、3.1、2.0Kb的4条杂交带,呈现了与W片段相同顺序的阳性区带,而鼻咽良性疾患和胎儿鼻咽组织DNA均为阴性,此结果提示在人鼻咽癌组织中,确实存在EB病毒基因组。

 
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