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线粒体游离钙
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  mitochondrial free calcium
     Effect of Yi-Xin-Kang Capsule and Dan Shen Tablet Containing Serum on Change of Mitochondrial Free Calcium and the Ability of Ca~(2+) Uptake Induced by Fe~(2+) /Ascorbic Acid in Rat Myocardium
     益心康胶囊和复方丹参片含药血清对Fe~(2+)/抗坏血酸致大鼠心肌线粒体游离钙及摄钙能力改变的影响
短句来源
     Objective: We studied the protective effects of Yi Xin Kang capsule (H303) and Dan Shen tablet (DS) containing serum,two kinds of traditional Chinese prescription,on mitochondrial injury of myocardium in rat. Method:The effects of drugs containing serum on mitochondrial free calcium and the ability of Ca 2+ uptake were evaluated by mean of oxygen stress induced by free radical generating system (Fe 2+ /Ascorbic acid) in vitro.
     目的 :研究益心康胶囊 (H30 3)和复方丹参片 (FD)对大鼠心肌线粒体损伤的保护作用。 方法 :利用体外自由基产生体系造成线粒体氧应激损伤 ,观察两药含药血清对线粒体游离钙及摄钙能力的影响。
短句来源
  “线粒体游离钙”译为未确定词的双语例句
     Result:The free Ca 2+ of mitochondria changed after mitochondria were injured by Fe 2+ /ascorbic acid(2.5/2.5mM) for 30min.
     结果 :2 5 2 .5mMFe2 + 抗坏血酸损伤心肌线粒体 30min后 ,可使线粒体游离钙及其摄钙能力发生不同的变化。
短句来源
     When comparison was made between 5 - HD + IP group and IP group, the increase of LVEDP, the lowering of LVDP and the increase of calcium content, were more marked in the former than in the latter (P < 0.01);
     5HD+IP组与IP组比较,再灌注各时间点大鼠心脏收缩功能指标LVDP有显著性差异(P<0.01),缺血末、再灌注末线粒体游离钙浓度显著高于IP组(P<0.01);
短句来源
     Effect of Exhaustive Exercise on Free Calcium and Phospholipase A2 Activity in Rat Myocardial Mitochondria
     力竭运动对大鼠心肌线粒体游离钙及磷脂酶A_2的影响
短句来源
     Results:The liver mitochondrial respiratory function、the activity of oxidase、free calcium concentration、liver Na +-K +-ATPase, serum free triiodinethyroxine(FT 3)、free tetra iodinethyroxine(FT 4)、testosterone(T)and hypothalamus NE content in “kidney yang deficiency” group were significantly decreased than the “kidney yin deficiency” group, mitochondrial MDA content of “kidney yang deficiency”, however, was higher obviously.
     结果 :老年“肾阳虚”鼠RCR、P O、OPR、细胞色素C氧化酶、Na+-K+-ATP酶活性、线粒体游离钙和血清T、FT3、FT4 及下丘脑NE含量均低于“肾阴虚”鼠 ,而“肾阳虚”组MDA含量却明显升高。
短句来源
     Objective: To explore the mechanisms of hypoxia pulmonary artery constriction(HPV) by researching the fluorescence intensity change of mitochondrial [Ca~(2+) ] of pulmonary artery smooth muscle cell(PASMC). Methods: PASMCs from adult Wister rats were isolated,cultured and identified.
     目的:通过观察肺动脉平滑肌细胞(pulmonary artery smooth muscle cells,PASMCs)在化学缺氧、细胞膜去极化和血管舒缩活性物质等不同因素作用下线粒体游离钙([Ca2+]mit)的动态变化特点,探讨:[Ca2+]mit与缺氧性肺动脉收缩(hypoxia pulmonary artery constriction,HPV)发生之间的关联。
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  相似匹配句对
     ANTIHYPERTENSIVE DRUGS AND PLATELETS IONIZED CALCIUM
     抗高血压药与血小板游离
短句来源
     Berberine-induced Changes of Mitochondria Membrane Potential and Free Intracellular Calcium of HaCaT Cells
     盐酸小檗碱对HaCaT细胞内游离离子及线粒体膜电位的影响
短句来源
     The activities of SOD,ATPase and contents of MDA,concentration of free Ca~(2+) in the brain mitochondria of rats were detetmined.
     测定脑线粒体中SOD、ATPase活性和MDA含量、基质游离浓度。
短句来源
     Mitochondria and Calcium Homeostasis.
     线粒体和细胞内自稳平衡
短句来源
     Objective:To explore the role of mitochondria calcium.
     目的本文旨在探讨线粒体
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  mitochondrial free calcium
Mitochondrial free calcium regulation in hypoxia and reoxygenation: relation to cellular injury
      
Specifically, mROS and mitochondrial free calcium (mCa2+) were simultaneously measured from cells received subcellular PDT with BPD-MA in different compartments including the mitochondrion, the cytosol and the nucleus.
      
Parkin does not prevent the ceramide-induced increase in mitochondrial free calcium levels.
      


Objective To observe the protective effects of ischemic preconditioning on the mitochondria (Mi)and energy metabolism in immature rat myocardial cells under ischemic condition followed by reperfusion. Methods Isolated working heart models were established using 4-weed-old rats, divided into 3 groups (12 in each group). The control rats (NC group) underwent 120 min of gllobal ischemia followed by 60 min of reperfusion with Krebs-Henseleit(K-H) solution at 37 ℃. The rats subjected to cardioplegic arrest with cold...

Objective To observe the protective effects of ischemic preconditioning on the mitochondria (Mi)and energy metabolism in immature rat myocardial cells under ischemic condition followed by reperfusion. Methods Isolated working heart models were established using 4-weed-old rats, divided into 3 groups (12 in each group). The control rats (NC group) underwent 120 min of gllobal ischemia followed by 60 min of reperfusion with Krebs-Henseleit(K-H) solution at 37 ℃. The rats subjected to cardioplegic arrest with cold (4 ℃) St.Thomas Ⅱ Hospital Solution before prolonged global ischemia and reperfusion served as ST group. The rats in ischemic preconditioning group (PI group) was given the same treatment as ST group after 5 min of global ischemia followed by 5 min of reperfusion at 37 ℃ with K-H solution, Malodiadehyde (MDA), free calcium(Ca2+) and superoxide dismutase(SOD) content in the mitochodria and myocardial ATP level were assessed before ischemia and during reperfusion. In addition, the cardiacfunction was measured and changes in the ultrastructures of the myocardial cells observed. Results The recovery of left ventricular function was much better in PI group than in NC and ST groups(P<0.05). MDA and Ca2+ content in the mitochondria were markedly lowered and SOD level elevated in PI group compared with those in the other 2 groups (P<0.05). The myocardial ATP content was markedly higher in PI and ST group. Slight ultrastuctural changes of the mitochondria were observed in IP group,in contrary to the condition observed in NC and ST group. Conclusion ischemic preconditioning may provide protection in addition to the effect of crystollid cardioplegic ischemia on immature myocardium against reperfusion injury.The mechanisms by which ischemic preconditioning protects the myocardium may lie in the decrease of oxgen free radical and Ca2+ overload in the mitochondria and the preservation of ATP as well as the maintance of integration of myocardial and mitochondria ultrastructure.

目的研究内源性心肌保护机制一缺血预适应(Ischemic-preconditioning,IPC)对未成熟心肌线粒体及能量代谢的 影响,并探讨其作用机制。方法采用改良Langendorff-Neely离体鼠心灌流装置,建立未成年大鼠(4周龄)离体心脏顺 行灌注左心作功模型。36只大鼠随机等分为缺血对照组(NC)、St.ThomasⅡ晶体停搏液组(ST)、预缺血加停搏液组 (PI)。比较再灌注后各组心功能(LWSP,LVEDP,±dp/dtmax,CO)的恢复率;测定缺血前、再灌注后线粒体游离钙(Ca2+)、 丙二醛(MDA)、超氧化物歧化酶(SOD)及心肌ATP含量;观察再灌注后心肌及线粒体超微结构的变化。结果 IP组心 功能指标的恢复率明显好于ST组和NC组(P<0.05);再灌注后ST组、NC组心肌线粒体Ca2+、MDA含量明显增高, SOD含量明显减少(P<0.05),而 IP组则无明显变化;IP组、ST组 ATP含量无显著差别,均明显高于 NC组;ST组、NC 组心肌及线粒体超微结构破坏明显,IP组则仅有轻微改变。结论St.ThomasⅡ晶体停搏液能减轻未成熟心肌能量损耗, 但对心肌线粒体...

目的研究内源性心肌保护机制一缺血预适应(Ischemic-preconditioning,IPC)对未成熟心肌线粒体及能量代谢的 影响,并探讨其作用机制。方法采用改良Langendorff-Neely离体鼠心灌流装置,建立未成年大鼠(4周龄)离体心脏顺 行灌注左心作功模型。36只大鼠随机等分为缺血对照组(NC)、St.ThomasⅡ晶体停搏液组(ST)、预缺血加停搏液组 (PI)。比较再灌注后各组心功能(LWSP,LVEDP,±dp/dtmax,CO)的恢复率;测定缺血前、再灌注后线粒体游离钙(Ca2+)、 丙二醛(MDA)、超氧化物歧化酶(SOD)及心肌ATP含量;观察再灌注后心肌及线粒体超微结构的变化。结果 IP组心 功能指标的恢复率明显好于ST组和NC组(P<0.05);再灌注后ST组、NC组心肌线粒体Ca2+、MDA含量明显增高, SOD含量明显减少(P<0.05),而 IP组则无明显变化;IP组、ST组 ATP含量无显著差别,均明显高于 NC组;ST组、NC 组心肌及线粒体超微结构破坏明显,IP组则仅有轻微改变。结论St.ThomasⅡ晶体停搏液能减轻未成熟心肌能量损耗, 但对心肌线粒体的保护作用较小,影响了?

Objective:To research on the energy metabolism of mitochondris and its regulation of aging rats with “deficiency of Kidney Yin or Kidney Yang”. Methods:According to the post pedes temperature, the SD senile rats were divided into “kidney yang deficiency” group、control、“kidney yin deficiency” group. The liver mitochondrial respiratory function the activity of oxidase MDA and free calcium concentration liver Na +-K +-ATPase, serum free triiodinethyroxine(FT 3)、free tetra iodinethyroxine(FT 4)、testosterone(T)、hypothalamus...

Objective:To research on the energy metabolism of mitochondris and its regulation of aging rats with “deficiency of Kidney Yin or Kidney Yang”. Methods:According to the post pedes temperature, the SD senile rats were divided into “kidney yang deficiency” group、control、“kidney yin deficiency” group. The liver mitochondrial respiratory function the activity of oxidase MDA and free calcium concentration liver Na +-K +-ATPase, serum free triiodinethyroxine(FT 3)、free tetra iodinethyroxine(FT 4)、testosterone(T)、hypothalamus NE content and heart rate、were estimated by the oxygen electrode method and radioimmunoassay etc. Results:The liver mitochondrial respiratory function、the activity of oxidase、free calcium concentration、liver Na +-K +-ATPase, serum free triiodinethyroxine(FT 3)、free tetra iodinethyroxine(FT 4)、testosterone(T)and hypothalamus NE content in “kidney yang deficiency” group were significantly decreased than the “kidney yin deficiency” group, mitochondrial MDA content of “kidney yang deficiency”, however, was higher obviously. Conclusion:The energy production and energy expenditure of “kidney yang deficiency” group were lower, oxidative damage were more serious than “kidney yin deficiency” group and was correlated to the mitochondrial self regulation and neuro endocrinal regulation.

目的 :观察老年期肾阴阳虚损大鼠的线粒体能量代谢变化及其调控机制。方法 :将 2 4月龄大鼠按掌温高低区分为老年“肾阴虚”(掌温高 )、老年对照 (常温 )和老年“肾阳虚”(掌温低 )三组 ,采用氧电极法和放免法等技术观察各组老年鼠肝线粒体呼吸功能、氧化酶活性、游离钙、MDA浓度、肝Na+-K+-ATP酶活性和血清FT3、FT4 、T、下丘脑NE含量以及心率等变化。结果 :老年“肾阳虚”鼠RCR、P O、OPR、细胞色素C氧化酶、Na+-K+-ATP酶活性、线粒体游离钙和血清T、FT3、FT4 及下丘脑NE含量均低于“肾阴虚”鼠 ,而“肾阳虚”组MDA含量却明显升高。结论 :老年“肾阳虚”鼠线粒体能量代谢低下 ,氧化损伤加剧 ,可能与神经内分泌调控及线粒体微观失调有关

Objective: We studied the protective effects of Yi Xin Kang capsule (H303) and Dan Shen tablet (DS) containing serum,two kinds of traditional Chinese prescription,on mitochondrial injury of myocardium in rat. Method:The effects of drugs containing serum on mitochondrial free calcium and the ability of Ca 2+ uptake were evaluated by mean of oxygen stress induced by free radical generating system (Fe 2+ /Ascorbic acid) in vitro. Result:The free Ca 2+ of mitochondria changed after mitochondria...

Objective: We studied the protective effects of Yi Xin Kang capsule (H303) and Dan Shen tablet (DS) containing serum,two kinds of traditional Chinese prescription,on mitochondrial injury of myocardium in rat. Method:The effects of drugs containing serum on mitochondrial free calcium and the ability of Ca 2+ uptake were evaluated by mean of oxygen stress induced by free radical generating system (Fe 2+ /Ascorbic acid) in vitro. Result:The free Ca 2+ of mitochondria changed after mitochondria were injured by Fe 2+ /ascorbic acid(2.5/2.5mM) for 30min. The ability of Ca 2+ uptake decreased significantly,from 73.1% to 30.8%. The abilities of Ca 2+ uptake in the groups of H303 and DS containing serum(50~800 mg/kg) were highter than that in injured control group. Conclusion:Free radical may cause the changes of free Ca 2+ and ability of Ca 2+ uptake of myocardial mitochondria in rat,drugs containing serum possessed protective effects on mitochondrial injury.

目的 :研究益心康胶囊 (H30 3)和复方丹参片 (FD)对大鼠心肌线粒体损伤的保护作用。方法 :利用体外自由基产生体系造成线粒体氧应激损伤 ,观察两药含药血清对线粒体游离钙及摄钙能力的影响。结果 :2 5 2 .5mMFe2 + 抗坏血酸损伤心肌线粒体 30min后 ,可使线粒体游离钙及其摄钙能力发生不同的变化。线粒体摄钙能力明显降低 ,由正常血清对照组的 73 1%降至 30 8%。H30 3及FD( 50~ 80 0mg kg)含药血清组可使钙摄取能力有不同程度的增加。结论 :自由基损伤可引起大鼠心肌线粒体游离钙及摄钙能力改变 ,H30 3和FD含药血清对此具有保护作用

 
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