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猪肾细胞
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  porcine kidney cell
     Furthermore,apoptosis of the porcine kidney cell line PK-15 % PK-IBRS2 cells infected with hog cholera lapinized virus (HCLV), GXWZ, SM strain of CSFV in vitro was studied in this paper. The cells were collected at different time post-infection and analyzed by DNA agarose gel electrophoresis.
     同时,我们对猪瘟病毒体外诱导细胞凋亡进行了初步的研究。 用猪瘟病毒广西梧州株(GXWZ)、石门株(SH!MEN)、猪瘟兔化弱毒疫苗株(HCLV)分别在体外感染猪肾细胞(PK一15和PK一旧RSZ),于感染后不同时间收集接毒组与对照组的细胞,抽提细胞核DNA进行检测。
短句来源
  porcine kidney cells
     Construction of recombinant retroviral vector carrying porcine interferon-gamma and its expression in porcine kidney cells (PK-15)
     携带猪γ干扰素基因逆转录病毒载体的构建及其在猪肾细胞(PK-15)中的表达
短句来源
     Study on the Comparison of Expressing Characteristics of Classical Swine Fever Virus and Full-length cDNA in Porcine Kidney Cells
     猪瘟病毒及其全长cDNA在猪肾细胞中增殖表达特性的比较研究
短句来源
  pig kidney cell
     A porcine circovirus type 2(PCV2) was isolated from a piglet lymph node material with postweaning multsystemic wasting syndrome(PMWS) using PCV1-free pig kidney cell line(PK15). The isolate was cultivated by a series of passages in the cells,and a cell culture-adapted strain was obtained,which was termed as PCV2/LG.
     从临床表现为仔猪断奶后多系统衰竭综合征(PMWS)淋巴组织病料,经聚合酶链式反应(PCR)证实为猪圆环病毒2型(PCV2)感染,采用无污染的猪肾细胞系(PK15)分离培养,并连续传代培育成一株细胞培养适应毒,命名为PCV2/LG株。
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  “猪肾细胞”译为未确定词的双语例句
     Pocine kidney cells and PK-15 cells were infected by the supernatant and were selected by 400μg/mL, 600μg/mL and 800μg/mL G418, respectively.
     将上清感染猪肾细胞(PK-15),经含G418(400μg/mL、600μg/mL和800μg/mL)的DMEM筛选一周,间接免疫荧光表明表达的猪γ干扰素主要锚定于细胞膜。
短句来源
     Swine foot-and-mouth disease virus﹙FMDV﹚﹙O/GD/MSH/86﹚were adapted to different cells, MDBK, BHK-21 and PK-15, the host tropism of FMDV was studied.
     将猪FMDV毒株(O/GD/MSH/86)分别适应牛肾细胞(Bovinekidney,MD-BK)、幼仓鼠肾细胞(Babyhamsterkidney,BHK-21)和猪肾细胞(Porcinekidney,PK-15),研究了FMDV在宿主细胞上的嗜性。
短句来源
     The HL-LY strain of Classical Swine Fever Virus(CSFV) was propagated and harvested on pk-15.One pair of primers were designed to amplify E 2 gene by RT-PCR according to published sequence of CSFV's E 2 gene of gene bank .
     用猪肾细胞 (pK - 15 )繁殖猪瘟病毒 (CSFV)分离株HL -LY ,根据基因库已发表的CSFVE2 基因序列 ,设计并合成一对引物 ,以CSFV总RNA为模板 ,通过RT -PCR技术扩增出约 1.1kb的片段 ,即E2 基因。
短句来源
     The open reading frames for gag, pol, and env in PERV-MSL have over 99% homological amino acid sequence identity to those of Tsukuba-1 retrovirus and are highly homologous to those of endogenous retrovirus of cell line PK15 (PK15-ERV).
     PERV-MSL的gag、pol、env的ORF氨基酸序列与Tsukuba-1逆转录病毒氨基酸序列的同源性>99%,与胎猪肾细胞PERV(PK15-PERV)氨基酸序列具有高度同源性。
短句来源
     The HL- L Y strain of classical swine fever virus(CSFV) was propagated and harvested on pk- 15 .One pair of primers were designed to amplify E2 gene by RT- PCR according to published sequence of CSFV's c DNA.
     用猪肾细胞 (PK- 15 )繁殖猪瘟病毒 (CSFV)分离株 HL - L Y,根据基因库已发表的 CSFV E2 基因序列 ,设计并合成一对引物 ,以 CSFV总 RNA为模板 ,通过 RT- PCR技术扩增出约 1.1kb的片段 ,即 E2 基因。
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      porcine kidney cell
    Establishment and characterization of a porcine kidney cell line, FS-L3, which forms unique multicellular domes in serum-free cu
          
    Characteristics of the porcine kidney cell line IB-RS-2 clone D10 (IB-RS-2 D10) which is free of hog cholera virus
          
    A Brazilian stock of clone C17 of the IB-RS-2 porcine kidney cell line which was contaminated with hog cholera virus (HCV) was cloned.
          
    The experimental production of a formalin-killed Japanese encephalitis virus vaccine from porcine kidney cell cultures
          
    The viral RNA species synthesized in a porcine kidney cell line, PS(Y-15), by Japanese encephalitis virus (JEV) are described.
          
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      porcine kidney cells
    We investigated the transfection efficiency of naked plasmid DNA in porcine kidney cells by applying holmium laser (Ho:YAG) energy in vitro as well as ex vivo in a porcine kidney papilla model.
          
    Porcine kidney cells were incubated in medium with or without 10 μg/mL of triol for 24 h, then incubated for 1, 6, or 12 h in a medium which contained 50 μM of either [14C]linoleic acid or unlabeled linoleic acid.
          
    MDP and LPS were toxic to rabbit and monkey kidney cells, MDP was toxic to canine kidney cells, but not to human or porcine kidney cells.
          
    An improved method of assay for human adenovirus type 12 (prototype strain) employing a clonal line of porcine kidney cells
          
    Methods for increasing the susceptibility of primary cultures of porcine kidney cells to infection with foot-and-mouth disease v
          
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      pig kidney cell
    Moreover, CD69 mRNA is expressed in bone marrow, spleen, thymus and lymph nodes but not in muscle, mammary gland, or the pig kidney cell line (LLC-PK1).
          
    Chromosomal banding patterns and karyotype evolution in three pig kidney cell strains (PK15, F and RP)
          
    Some of the techniques used to obtain banding patterns in human karyotype are adapted here to three pig kidney cell strains (PK15, F and RP).
          
    Transepithelial transport in cell culture:d-Glucose transport by a pig kidney cell line (LLC-PK1)
          
    The pig kidney cell line LLC-PK1 cultured on a collagen coated membrane filter formed a continuous sheet of oriented asymmetrical epithelial cells joined by occluding junctions.
          
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    Summary 1.The cell-cultured lapinized strain of swine fever virus was sucessfully propagated on PK primary cell monolayer in rolling bottles, LHHank's solution containing horse or calve serum was used as the medium and no antibiotics were added. The inoculum was a 0.3% of 1:10 spleen suspension of infected rabbit or a 2—4% cell cultured virus. After cultivation at 36℃ the media were havested and changed every 4 days. The titre of havested virus in every run were 2x10~4-5x10~4 in terms of rabbit infective titre....

    Summary 1.The cell-cultured lapinized strain of swine fever virus was sucessfully propagated on PK primary cell monolayer in rolling bottles, LHHank's solution containing horse or calve serum was used as the medium and no antibiotics were added. The inoculum was a 0.3% of 1:10 spleen suspension of infected rabbit or a 2—4% cell cultured virus. After cultivation at 36℃ the media were havested and changed every 4 days. The titre of havested virus in every run were 2x10~4-5x10~4 in terms of rabbit infective titre. 2.Each dose of the lyophylized combined vaccine contains 0.015 ml cell-cuture lapinized strain of swine fever virus.70 batches of such vaccines have been tested for their protective ability against swine fever. All vaccinated pigs have been challenged with swine fever virus and protection was shown to be 100%, and they have been protected against swine erysipelas and swine pasteurellosis aswell. More than one thousand million pigs were vaccinated in the fields. and results of vaccination proved to be safe and satisfactory.

    用转瓶培养猪肾细胞生产猪瘟细胞苗,本试验证明①用小黄牛血清或马血清或犊牛血清均可制出较高毒价的猪瘟细胞毒,②营养液中加青、链霉索各90—100μ/ml,接毒后维持液不用抗菌素,可以控制污染,並有利于毒的复制,③按营养液量接种猪瘟兔化毒脾毒0.3%(克)或2—4%细胞培养毒,均可制出毒价5×10~(-4)的细胞苗,但必须保持收液pH在6.8—7.0左右,④残存抗菌素在0.5μ/ml以下的细胞毒,配三联苗后对猪丹毒及猪肺疫菌影响不显著,⑤已连续制三联苗70余批,三种成份分别检验均达到规定标准,田间试使已接种猪1000万头以上,安全有效。

    1. By cultivating the lapinized hog cholera vaccine virus(LHCV) on pig kidney cells in rolling bottles with increased volume of cultural media(3—4 times more than the amount used in the routine method), the titres in the first and second harvests of vaccine virus were quite similar to those obtained by the routine method. The titres in the second harvest passed most of the tests of 5×10~4 RID/1ml (Rabbit Infective Doses). Results of the tests met entirely the demand for the mass production of vaccine. 2. The...

    1. By cultivating the lapinized hog cholera vaccine virus(LHCV) on pig kidney cells in rolling bottles with increased volume of cultural media(3—4 times more than the amount used in the routine method), the titres in the first and second harvests of vaccine virus were quite similar to those obtained by the routine method. The titres in the second harvest passed most of the tests of 5×10~4 RID/1ml (Rabbit Infective Doses). Results of the tests met entirely the demand for the mass production of vaccine. 2. The method of cultivating hog cholera vaccine virus in rolling bottles around an inclined axis and with deeper culture media(7—8 times more than in the routine method) produced titres in the first and second harvests or in a single harvest(7 days after inoculation) quite similar to those obtained by the routine method.They fitted well the requirements for vaccine production. 3. We also cultivated the LCHV in rolling bottles as cited above but with even much more culture media(8 times more) and additional aerati on. In this case the titres obtained in single harvests(7 days after inoculation)passed most of the 5×10~4RID/1 ml tests also. The data given above suggested that these three methods of cultivation might be applicable in actual vaccine production, with the only consideration that much greater amounts of culture media than used in the routine method of production had to be consumed in these cases. These modifications would much simplify the production of LCHV vaccine.

    1、用增加培养液量(3—4倍)旋转法培养猪瘟弱毒细胞疫苗,第一收、第二收病毒收获液的毒价均可达到普通旋转培养法的滴度。二收的绝大多数批次毒价用兔检5万倍通过,符合制苗要求。 2、用深液(培养液量增加7—8倍)倾斜旋转法培养猪瘟弱毒细胞疫苗。第一收与第二收的毒液或培养7天一次全收的毒液,其大多数试验批次毒价均可达到制苗标准。 3、用深液(培养液量增加8倍)倾斜旋转通气法培养猪瘟弱毒细胞疫苗,接毒后培养7天一次全收毒液,大多数批次试验毒价用兔检5万倍通过。符合制苗要求。以上三种培养方法所得资料说明提高猪肾细胞产毒量是明显的,工艺是简便的,在生产上是可行的。对细胞提高繁殖病毒能力的问题进行了某些探索。

    In view of the fact that vaccination of hogs against hog cholera with the lapinized hog cholera virus(LHCV)produced in hog kidney cell (HKC)cultures has the danger of inducing diseases in hogs by patho- gens originated from hog kidneys,we decided to conduct experiments on the cultivation of LHCV in goat testis(GT)and goat kidney(GK) cell cultures.The results showed that both GT and GK cell cultures could support the propagation of LHCV which was produced continuously wi- thout inducing CPE,the harvest of the...

    In view of the fact that vaccination of hogs against hog cholera with the lapinized hog cholera virus(LHCV)produced in hog kidney cell (HKC)cultures has the danger of inducing diseases in hogs by patho- gens originated from hog kidneys,we decided to conduct experiments on the cultivation of LHCV in goat testis(GT)and goat kidney(GK) cell cultures.The results showed that both GT and GK cell cultures could support the propagation of LHCV which was produced continuously wi- thout inducing CPE,the harvest of the virus-containing fluid could be carried out more than once,and the primary cultures of GT and GK cells could be subcultured.The subcultured cells appeared to possess greater adaptibility to the environment than the primary culture because the cell sheet could maintain longer and still support the propagation of the virus. The virus titres of the harvested fluids from both the primary and the subcultured cultures were shown to be high enough to conform to the requirements of regular vaccine production. Experiments on the potency and safetiness of the subcultured virus were successfully performed,and it is suggested that it would he possible to utilize GTand GK cells instead of HKC in the production of hog cholera vaccine.

    针对目前使用猪肾细胞生产猪瘟弱毒疫苗存在来自组织本身污染带来的潜在致病危险的情况,我们试验了用羊羔睾丸与肾细胞来生产猪瘟病毒。结果表明:猪瘟兔化弱毒适应羊羔睾丸与肾细胞,呈温和感染而不致细胞病变,可连续收获多次,产毒较高,毒价稳定。将原代培养的羊羔睾丸、肾细胞传一代后,细胞更加适应体外环境,维持时间较长,经再接毒后产毒效果仍然很好,均可达生产要求。由此看来,用羊羔睾丸、肾细胞代替猪肾细胞生产猪瘟病毒苗头很好,若进一步试验成功,可考虑用于生产。

     
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