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   类固醇合成快速调节蛋白mrna 的翻译结果: 查询用时:0.007秒
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类固醇合成快速调节蛋白mrna
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     HCG Acutely Regulating Testosterone's Biosynthesis by Increasing the Expressing of StAR Protein
     HCG通过StAR蛋白快速调节睾酮的合成
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     Advances in the Study of Steroidogenic Acute Regulatory Protein
     类固醇激素合成急性调节蛋白研究进展
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     Regulation of steroid involved c-fos mRNA and c-fos expression in painful model of the rat
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     CALCIUM REGULATED PROTEINS
     钙调节蛋白
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To study the mechanism of changes of the ability of testosterone synthesis in different periods , Kunbai mouse in different periods were used as experimental animals . The ability of testosterone synthesis was analyzed by RT-PCR and Western blot.The results were as followed: (1) In all periods, the expression of cholesterol side chain decomposition enzyme (P450scc) , 3 beta-hydroxysteroid dehydrogenase(3β-HSD),steroidogenic acute regulatory protein (StAR) mRNA and protein had no significant change.(2)The levels...

To study the mechanism of changes of the ability of testosterone synthesis in different periods , Kunbai mouse in different periods were used as experimental animals . The ability of testosterone synthesis was analyzed by RT-PCR and Western blot.The results were as followed: (1) In all periods, the expression of cholesterol side chain decomposition enzyme (P450scc) , 3 beta-hydroxysteroid dehydrogenase(3β-HSD),steroidogenic acute regulatory protein (StAR) mRNA and protein had no significant change.(2)The levels of cytochrome P450 17 alpha-hydroxylase (P450c17)mRNA were high in 10,60 and 120 d. However, that of P450c17 mRNA decreased significantly in 270 d. (3) From 30 d to 270 d, the activity of extracellular signal regulated kinases (ERK) had no significant change. These results inferred that the difference of the level of P450c17 mRNA was an important factor of the difference of the level of testosterone,but the activity of ERK had no direct relation with the decrease of P450c17 mRNA and the level of testo sterone

不同发育阶段,睾丸合成睾酮的能力差异很大。为了阐明引起睾酮合成差异的分子机制,实验以不同年龄的SPF昆白小鼠,采用RT-PCR与Western blot分析了不同年龄阶段类固醇合成酶的变化。结果显示:(1)在不同年龄阶段,P450scc(胆固醇支链裂解酶)mRNA、3β-HSD(3β-羟胆固醇脱氢酶)mRNA、StAR(类固醇合成快速调节蛋白)mRNA和蛋白的水平无明显变化;(2)P450c17(C17-20裂解酶)mRNA的表达水平在30、60和120d均处于较高的水平,但是在270d则处于较低的水平;(3)30~270d,细胞外信号调节激酶(ERK)活性无明显变化。表明,P450c17 mRNA表达差异可能导致不同时期睾酮合成能力差异的重要原因,但ERK活性可能与衰老引起睾酮合成能力下降无直接关系。

 
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