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   粪样细菌 的翻译结果: 查询用时:0.211秒
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粪样细菌
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  “粪样细菌”译为未确定词的双语例句
     The regions V6 to V8 of the 16S rDNA of faecal bacteria were amplified. DGGE profiles of the PCR amplicons were compared by similarity analysis.
     粪样细菌 1 6SrDNA的V6~V8可变区经PCR扩增 ,扩增产物经DGGE电泳后再进行相似性分析。
短句来源
     The microbial count result showed that the numbers of total bacteria and vibrios in the samples increased in the order of longshore water,pond water and fecal samples.
     传统的微生物培养计数表明,从海湾水、养殖池水到对虾粪样,细菌和弧菌的数量表现出依次增加的趋势,粪样及肠壁中弧菌的数量高出外界水环境1—4个数量级。
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  相似匹配句对
     APXIVA gene couldn't be detected from dung.
     中不能检测到该基因。
短句来源
     Feces Analysis and Its Role in Wildlife Research
     在野生动物研究中的作用
短句来源
     Sample Test
     (题)
短句来源
     coli 24 h, 48 h.
     coli后24h,48h细菌的数量;
短句来源
     Analysis of calves' excrement with Denaturing Gradient Get Electrophoresis(DGGE);
     细菌区系的变性梯度凝胶电泳(DGGE)分析;
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PCR and denaturing gradient gel electrophoresis (DGGE) were used to monitor the deve- lopment of bacterial community in faeces of 12 weaning piglets. The regions V6 to V8 of the 16S rDNA of faecal bacteria were amplified. DGGE profiles of the PCR amplicons were compared by similarity analysis. Results revealed simple DGGE profiles for faecal samples from piglets on the first day of weaning and piglets from the same sow showed similar DGGE profiles. After weaning as the piglets grew DGGE profiles became complicated...

PCR and denaturing gradient gel electrophoresis (DGGE) were used to monitor the deve- lopment of bacterial community in faeces of 12 weaning piglets. The regions V6 to V8 of the 16S rDNA of faecal bacteria were amplified. DGGE profiles of the PCR amplicons were compared by similarity analysis. Results revealed simple DGGE profiles for faecal samples from piglets on the first day of weaning and piglets from the same sow showed similar DGGE profiles. After weaning as the piglets grew DGGE profiles became complicated and diverse. Each individual piglet had its unique DGGE profile, with low similarity between piglets. The source of sow and the diet showed no appa- rent effect on the DGGE profiles. For the piglets fed a diet containing oliogofructose, DGGE similarity analysis showed bacterial community in the faeces developed fast during the first week and relatively slow later after weaning.

利用PCR和DGGE技术分析了 1 2头仔猪在断奶后其粪样细菌区系的变化。粪样细菌 1 6SrDNA的V6~V8可变区经PCR扩增 ,扩增产物经DGGE电泳后再进行相似性分析。结果表明 ,仔猪断奶当天粪样DGGE谱带少 ,同窝仔猪间图谱相似。断奶后 ,随着断奶时间的推移 ,每头仔猪的DGGE图谱带逐渐增多 ,变得复杂和多样 ,仔猪个体间DGGE图谱差异逐渐增大。仔猪是否同窝以及所采食日粮类型对DGGE图谱没有明显影响。相似性分析还表明 ,日粮中添加寡果糖的仔猪在断奶后第 1周 ,其粪样微生物区系变化迅速 ,而后缓慢。

PCR and denaturing gradient gel electrophoresis(DGGE) were used to track the development of fecal bacterial community of 5 diarrhea piglets.Clone libraries were created from complete 16S rDNA of 42-day fecal sample of piglet 1,2 and 5. From the library,clones had their V6-V8 regions matched predominant bands on the DGGE gel were then sequenced and their 16S rDNAs subjected to an online similarity search.Results revealed that DGGE profiles from fecal samples of 5 piglets changed form simple to complex,and then...

PCR and denaturing gradient gel electrophoresis(DGGE) were used to track the development of fecal bacterial community of 5 diarrhea piglets.Clone libraries were created from complete 16S rDNA of 42-day fecal sample of piglet 1,2 and 5. From the library,clones had their V6-V8 regions matched predominant bands on the DGGE gel were then sequenced and their 16S rDNAs subjected to an online similarity search.Results revealed that DGGE profiles from fecal samples of 5 piglets changed form simple to complex,and then returned simple to complex,finally trended to be stable and diverse.DGGE profiles from day 2and 16 fecal samples of 5 piglets were simplest,with high similarity(84%).Their most predominant bands were E.coil.Their Shannon index were 1.38±0.55 and 1.97±0.26 respectively.DGGE profiles from day 10 of 5 piglets became quite complex,and had quite low similarity(10%) with DGGE profiles from day 2and 16 fecal samples of 5 piglets.The Shannon index was 2.12±0.24.E.coil still existed,but it was not the most predominant band.There were significant differences of predominant bands between DGGE profiles from day 27 fecal samples and those from day 35 and 42 fecal samples,with 26.28% and 39.2% of similarity respectively.The predominant bands from day 35 and 42 fecal samples of 5 piglets were trended to be stable,with 55% of similarity.The Shannon index of day 27,day 35 and 42 fecal bacterial communities were 2.19±0.29,2.20±0.17and 2.14±0.20 respectively.The fecal bacterial communities of day 42 health piglets were mainly consisted of species of Enterococcus,Streptococcus,Clostridium,Peptostreptococcus and Lactobacillus.

用PCR/DGGE技术跟踪一窝5头新生腹泻仔猪自然康复、补饲、断奶过程中粪样细菌区系的演变,构建3头仔猪42日龄粪样的16S rDNA克隆库,分析匹配于DGGE优势谱带23个克隆的16S rDNA序列。结果表明,DGGE图谱由简单(2日龄)到复杂(10日龄),再回复简单(16日龄)到复杂(断奶),最后趋于稳定。2、16日龄DGGE图谱最简单、相似,最优势谱带为大肠杆菌;10日龄(补饲后3天)图谱复杂,大肠杆菌存在但不是最优势谱带,补饲前后图谱的相似性低,补饲导致了粪样细菌区系结构的显著变化;断奶前(27日龄)和后(35、42日龄)图谱复杂,优势谱带、图谱相似性均趋向稳定。序列分析表明,23个克隆中除5个与未知细菌最相似外,其余最相似菌分属于肠球菌(Enterococcus),链球菌(Streptococcus),梭菌(Clostridium),消化链球菌(Peptostreptococcus)和乳酸杆菌(Lactobacillus)。

A total of 34 weaning piglets from six litters were randomly assigned into two groups, offered basal diet(control) and basal diet plus manno-oligosaccharide (MOS), respectively. Faecal samples were collected on day 1(before first feeding), 7, 14, 21 and 28 after weaning. DNA was extracted from the faecal samples and the V6~V8 regions of bacterial 16S rDNA were amplified. Amplicons were analyzed by DGGE to investigate the diversity of the bacterial communities of weaning piglets. Results showed that piglets from...

A total of 34 weaning piglets from six litters were randomly assigned into two groups, offered basal diet(control) and basal diet plus manno-oligosaccharide (MOS), respectively. Faecal samples were collected on day 1(before first feeding), 7, 14, 21 and 28 after weaning. DNA was extracted from the faecal samples and the V6~V8 regions of bacterial 16S rDNA were amplified. Amplicons were analyzed by DGGE to investigate the diversity of the bacterial communities of weaning piglets. Results showed that piglets from the same litter had a higher similarity(81%~88%) of the bacterial community on day 1(before weaning) compared with that after weaning(61%~70%). DGGE similarities of microbial community between neighboring time points, e.g. 1~7, 7~14, 14~21 and 21~28, decreased during the first week for all piglets, but the value in the control remained around 61%, while those in the MOS treatment gradually increased to 70%. Diversity analysis showed that after 7 days the value for the MOS treatment increased while that for the control decreased. On day 28 the diversity index of the bacterial community from the MOS diet was 1.52 and the control group was 1.38. The differences of the diversity between the two groups increased from 0.09 on day 1 to 1.14 on day 28. In agreement with diversity data, DGGE band numbers revealed that during the first week of weaning the average band numbers reduced for both groups. However, after 14 days average band number for piglets fed the MOS diet increased, while that from piglets fed the control diet decreased. The results demonstrated that weaning had caused rapid changes of the bacterial community of piglets and that MOS supplemented in the diet could result in a rapid development and consequently early establishment of a high diverse bacterial community after weaning.

来自6窝健康、体重相近的杜长大哺乳仔猪34头随机地分为两组,分别于断奶后饲喂基础日粮或基础日粮+甘露寡糖(MOS),收集仔猪断奶当天(实验前)及断奶后7、14、21和28d粪样,利用PCR/DGGE技术对粪样细菌16SrDNA的V6~V8可变区进行了分析,以研究日粮中添加MOS对断奶仔猪粪样菌群稳定性及多样性的影响。结果表明,断奶前同窝仔猪带谱相似性较高(81% ̄88%),但存在个体差异;断奶后同窝仔猪的相似性下降(61%~70%);断奶后7、14、21和28d相邻时间段之间,基础日粮组仔猪菌群相似性维持在61%左右,MOS组菌群相似性在断奶第一周下降后,逐渐增加至70%,说明断奶可引起菌群发生变化,而MOS具有使菌群快速稳定的作用。多样性结果显示,断奶7d后MOS组菌群多样性增加,基础日粮组下降,14d后两组的差异增加,28d时基础日粮组多样性为1.52,MOS组为1.38,差异由断奶当天的0.09上升至28d的1.14,但差异不显著(P>0.05);条带数分析获得类似结果,即断奶7d时两组条带数均减少,14d后MOS组条带数增加,对照组的条带数却减少,实验期内MOS组条带数均高于对照组。结果提...

来自6窝健康、体重相近的杜长大哺乳仔猪34头随机地分为两组,分别于断奶后饲喂基础日粮或基础日粮+甘露寡糖(MOS),收集仔猪断奶当天(实验前)及断奶后7、14、21和28d粪样,利用PCR/DGGE技术对粪样细菌16SrDNA的V6~V8可变区进行了分析,以研究日粮中添加MOS对断奶仔猪粪样菌群稳定性及多样性的影响。结果表明,断奶前同窝仔猪带谱相似性较高(81% ̄88%),但存在个体差异;断奶后同窝仔猪的相似性下降(61%~70%);断奶后7、14、21和28d相邻时间段之间,基础日粮组仔猪菌群相似性维持在61%左右,MOS组菌群相似性在断奶第一周下降后,逐渐增加至70%,说明断奶可引起菌群发生变化,而MOS具有使菌群快速稳定的作用。多样性结果显示,断奶7d后MOS组菌群多样性增加,基础日粮组下降,14d后两组的差异增加,28d时基础日粮组多样性为1.52,MOS组为1.38,差异由断奶当天的0.09上升至28d的1.14,但差异不显著(P>0.05);条带数分析获得类似结果,即断奶7d时两组条带数均减少,14d后MOS组条带数增加,对照组的条带数却减少,实验期内MOS组条带数均高于对照组。结果提示,断奶导致了菌群的变化,日粮中添加MOS可加速菌群多样和稳定。

 
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