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600 b transcript, which presumably corresponds to the excised rps12 intron RNA.
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The intron RNA consists of 2492 nucleotides which can be folded into a secondary structure with all the expected sequence motifs of subgroup-IIA1 introns (Michel et al.
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The Porphyra intron self-splices in?vitro, and generates both ligated exons and a full-length intron RNA circle.
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In some cases, a splicing factor (also referred to as maturase) is encoded within the intronic sequence and produced by translation of the (excised) intron RNA.
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Group I intron RNA was incubated with a pool of random sequence RNA, or with a pool of RNA molecules which had previously been enriched for viomycin-binding RNAs.
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