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再生完整植株
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  “再生完整植株”译为未确定词的双语例句
     Calli were induced from leaf explants cultured on the medium MS + BA 2 mg/L + NAA 0.2 mg/L. Bud shoots were induced from the calli cultured on the medium MS + BA 3 mg/L + NAA 0.1 mg/L, and rooted on the half-strength MS medium at the presence of BA 0.6 mg/L. The rooted plantlets survived in greenhouse.
     结果在MS+2mg/LBA+0.2mg/LNAA的培养基上诱导出愈伤组织,在MS+3mg/LBA+0.1mg/LNAA的培养基上诱导出芽,并在1/2MS+0.6mg/LNAA的培养基上诱导出根,从而获得再生完整植株,生根的小苗在温室生长良好。
短句来源
     Bud formation was induced using MS medium supplemented with 0.5mg/L BA and 0.2mg/L NAA. Development of shoots was achieved on a 1/2 MS medium containing 0.3mg/L BA and 0.05mg/L NAA. The medium used for inducing root formation was the same as the above,but with 0.5mg/L IBA and 20 mg/L PVP.
     本文详细介绍了钻天柳幼枝的腋芽再生完整植株的组织培养技术:基本培养基为MS培养基,初始培养基附加BA0.20+NAA0.20,芽形成培养基附加BA0.50+NAA0.20,枝形成增殖培养基为1/2MS+BA0.30+NAA0.05,生根培养基为1/2 MS+IBA 0.50+pvp20.00。
短句来源
     Normal somatic embryos were converted into plantlets by over 75% after maturation.
     经过成熟培养后,正常的体胚75%以上萌发再生完整植株
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     The apical meristems were excised from the seedlings of 40~60 hours and cultured on the MSB medium without/with hormone or active charcoal.
     将棉花种仁无菌培养40~60h,制备茎尖分生组织,培养在无激素MSB培养基上,直接再生完整植株
短句来源
     Derived from cellcospension of C type callus of Oxytropis leptophylla. After the roots were induced on medium (1/2MS+0. 4mg/L IBA), the shoots developed into whole plants.
     经NB12培养基诱导分化出芽,MI培养基(MS+IBA0.4mg/L)诱导生根,再生完整植株
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  相似匹配句对
     Regenerated plantlets were found stable ingenetic behavior.
     再生完整植株在遗传特性上是稳定的。
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     Plant Regeneration from Hypercotyl of Radish ( Raphanus sativus L.)
     从萝卜下胚轴再生完整植株
短句来源
     Study on plant regeneration from cotyledon explants of a melon cultivar 'Huangdanzi'
     甜瓜‘黄蛋子’子叶再生完整植株研究
短句来源
     PRODUCTION OF PLANTS FROM SOMATIC HYBRIDIZATION BETWEEN COMMON WHEAT AND MAIZE(ZEA MAYS L)
     普通小麦与玉米的体细胞杂交再生完整植株
短句来源
     PLANT REGENERATION OF SOYBEAN VIAORGANOGENESIS
     大豆再生植株的研究
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Protoplast, isolated from mesophyll cells of tobacco (Nicotiana tabacum var. Copus yeusuheku No. 4) through a "one-step procedure" with the enzyme made in China, was cultured by means of liquid-thin-layer culture. About fourteen days later, the medium was supplemented with fresh liquid in the absence of mannitol, so as to dec-rease the osmotic pressure to half of the original level. Then, protoplast was transferred to a solid medium without mannitol. The small callus, 1-2mm in diameter, was transferred to the...

Protoplast, isolated from mesophyll cells of tobacco (Nicotiana tabacum var. Copus yeusuheku No. 4) through a "one-step procedure" with the enzyme made in China, was cultured by means of liquid-thin-layer culture. About fourteen days later, the medium was supplemented with fresh liquid in the absence of mannitol, so as to dec-rease the osmotic pressure to half of the original level. Then, protoplast was transferred to a solid medium without mannitol. The small callus, 1-2mm in diameter, was transferred to the differentiation medium for regeneration of the intact plant. The plantlet was transferred to a pot to grew.Comparisons of various components of media were carried out. It was shown that a little change of the content of two macroelements and the increase of lac-talbumin hydrolysate and certain organic substances, would accelerate the cell di-vision and increase the frequency from about 50% in NT medium as control, up to more than 80%. Budding and the effect of the condition on culture were discussed.

以普通烟草的一种香料烟品种为材料,用国产酶一步法游离叶肉原生质体。以液体浅层静置培养的方法培养原生质体,14天左右添加一次新鲜的不含甘露醇的培养液,使渗透压减半。以后转入完全没有甘露醇的固体培养基里。将形成的直径为1—2毫米的小愈伤组织转至分化培养基,再生了完整植株。而后将苗转至花盆里能正常生长。 比较了原生质体培养基的不同成分,发现将大量元素中两个成分的量略作改变,并增加水解乳蛋白及一些有机成分能加速分裂速度,分裂频率由对照NT培养基的50%左右提高到80%以上。讨论了出芽现象及培养条件对培养的影响。

Embryoids were obtained from the pollen callus of grape. Afther they hald developed into young halploid plantlets on the solid culture medium, some epidermal cells of the mesocotyl.hypcotyl, leaf and the apper part of the root dedifferentiated to embryonic state end devided actively From one or two suck cells amulticellulur mass was formed on the surface and then developed into an embryoid which always grew toa plantlet, when slaughed off and cultured.

由葡萄(Vitis Vinisera L)花药愈伤组织产生的胚状体形成单倍体幼苗后,经4—5次继代培养,发现其表皮细胞具有形成胚状体和进一步发育成幼苗的能力。胚状体的表层也具有同样的能力。将这样的一棵幼苗,整株置于液体培养中,在黑暗中震荡培养一周,然后在光照下静止培养,可获得大量完整的小植栋。实验结果证明葡萄单倍体的体细胞具有再生完整植株的全能性。在植物组织和细胞培养中,通过胚状体再进一步形成幼植物具有繁殖数量多、成苗速度快和结构完整的特点。因此,对于胚状体发生的研究,已经引起人们广泛的注意。目前,在植物的组织培养中,记载有胚状体发生的植物已涉及到广泛的分类类群,产生胚状体的离体培养物也有多种多样。但是对于胚状体形态发生的详细研究报导甚少。在1965年,Konor和Nataraja利用石龙芮的下胚轴进行切片观察时,看到了一系列胚状体发生的组织学过程。1970年Backs-Hüseman,D等在胡萝卜的单细胞悬浮培养中,见到了游离单细胞发育成胚状体的过程,1974年,Sunderland等在烟草中,也报导了小孢子胚状体的发生过程。但是,在具有经济价值的木本果树中,尚无这方面的报导。本文以葡萄单倍体幼...

由葡萄(Vitis Vinisera L)花药愈伤组织产生的胚状体形成单倍体幼苗后,经4—5次继代培养,发现其表皮细胞具有形成胚状体和进一步发育成幼苗的能力。胚状体的表层也具有同样的能力。将这样的一棵幼苗,整株置于液体培养中,在黑暗中震荡培养一周,然后在光照下静止培养,可获得大量完整的小植栋。实验结果证明葡萄单倍体的体细胞具有再生完整植株的全能性。在植物组织和细胞培养中,通过胚状体再进一步形成幼植物具有繁殖数量多、成苗速度快和结构完整的特点。因此,对于胚状体发生的研究,已经引起人们广泛的注意。目前,在植物的组织培养中,记载有胚状体发生的植物已涉及到广泛的分类类群,产生胚状体的离体培养物也有多种多样。但是对于胚状体形态发生的详细研究报导甚少。在1965年,Konor和Nataraja利用石龙芮的下胚轴进行切片观察时,看到了一系列胚状体发生的组织学过程。1970年Backs-Hüseman,D等在胡萝卜的单细胞悬浮培养中,见到了游离单细胞发育成胚状体的过程,1974年,Sunderland等在烟草中,也报导了小孢子胚状体的发生过程。但是,在具有经济价值的木本果树中,尚无这方面的报导。本文以葡萄单倍体幼苗为材料,进行切片观察和培养,对葡萄胚状体的发生进行了初步的探讨。

In 1985, the authors flrst reported the success of whole plant regeneration from in vitro cotton (Gossypium hirsutm L. and G. barabadense L.) shoot tip (ca 3 mm) cultures. Subsequent studies progressed in two respects on the basis of improved culture media. Frist, at the basal part of the stem of the formerly regenerated plantlet, massive calli of embryonic nature were induced, from which several buds or green embryoids were formed, indicating the possiblity of mass production of regenerated plants. So far,...

In 1985, the authors flrst reported the success of whole plant regeneration from in vitro cotton (Gossypium hirsutm L. and G. barabadense L.) shoot tip (ca 3 mm) cultures. Subsequent studies progressed in two respects on the basis of improved culture media. Frist, at the basal part of the stem of the formerly regenerated plantlet, massive calli of embryonic nature were induced, from which several buds or green embryoids were formed, indicating the possiblity of mass production of regenerated plants. So far, 5 plantlets per original shoot tip were found within two months culsture. Second, on the nodes of the stem, adventitious buds and multipe shoots or clusters of buds appeared. It was found that the Sj-1 basic medium supplemented with low concentration of 2,4-D, median concentration of 2iP or KIN, and glucose or sucrose was better than MS medium to induce differentiation of callus and fromation of green proembryos or buds. Thus, the induction of batch production of plantlets from the shoot tip of Gossypium spp. requires a somewhat broad range of growth regulators.

1985年,我们通过人工培养陆地棉茎尖取得再生植株。近二年,在改进培养基后,取得了两方面的进展:1、再生苗基部产生大量胚性愈伤组织,转入分化培养基后,分化出大量再生植株;2、茎节腋间产生不定芽,形成多苗或丛芽。并取得一次再生完整植株或分茎叶和生根二阶段产生植株。发现基础培养基SJ-1附加2,4-D和2ip或KIN,葡萄糖或蔗糖,出现绿芽(头)频率较高于MS培养基。研究结果指出,陆地棉茎尖人工培养诱导批量成苗,要求营养和激素条件较宽,愈伤组织再生效率高,一个茎尖经2个月培养可产生5个苗。采用了海岛棉杂种茎尖培养,结果相似。

 
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