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人类绒毛膜促性腺激素
相关语句
  human chorionic gonadotropin
     TOXICOLOGY AND SAFETY STUDIES OF HUMAN CHORIONIC GONADOTROPIN (hCG) CONTRACEPTIVE VACCINE ON RHESUS MONKEY
     人类绒毛膜促性腺激素避孕疫苗的毒理学和免疫安全性研究
短句来源
     Testing human chorionic gonadotropin by magnetic antibody immunity assay and analysis of it's clinical meaning
     磁性分离酶联免疫技术检测人类绒毛膜促性腺激素及其临床意义分析
短句来源
     An artificial gene equivalent to 36 C-terminal peptide of human chorionic gonadotropin (hCG) β-subunit has been chemically synthesized through the application oligonucleotide synthesizer and the double strand DNA has heen successfully cloned into an expression vector, λgtll, and transfected into the host strain E. Coli Y1089, through recombinant phage λgtll hCG to produce a hybrid protein containing hCG-β-suhunit 36 C-terminal peptide (Called β-galactosidase-hCG).
     基因工程菌株产生的含人类绒毛膜促性腺激素(hCG)β亚单位C末端36肽的β-半乳糖苷酶杂交蛋白(简称β-galaclosidase-hCG),在未经解聚处理前免疫新西兰种白兔和昆明种小白鼠,其抗血清在免疫双扩散试验和对流免疫电泳中对hCG没有形成沉淀线作用。
短句来源
     Human Chorionic Gonadotropin(hCG)β-subunit C-terminal peptide containing hybrid protein(β-galactosidase-hCG)produced by the recombinant λgt 11 hCG in host strain E. coli Y1089 was isolated and purified by ammoniun sulfate precipitated,anti-β-galactosidase affinity column. Pooled sera from various experimental groups were tested for anti-β-hCG activity in assays of in vivo neutralizing activity.
     基因工程菌株产生的含人类绒毛膜促性腺激素(hCG)β亚单位 C 末端36肽的β-半乳糖苷酶杂交蛋白(简称:β-Galactosidasc-hCG),主动免疫昆明种小白鼠,能诱发特异抗 hCG 抗体的产生。
短句来源
  “人类绒毛膜促性腺激素”译为未确定词的双语例句
     Study on the Immunogenicity of β-galactosidase-hCG Polymer Containing hCG-β-subunit C-terminal Peptide Synthesized in λgtll hCG Y1089
     基因工程菌株产生含人类绒毛膜促性腺激素抗原的研究 Ⅱ.β—galactosidase—hCG聚体蛋白免疫原性
短句来源
     Study on the immunological and Biological Activity Asay of β-Galactosidase-hCG containing hCG β-subunit C-terminal Peptitle Synthesized in λgt 11 hCG Y1089
     基因工程菌株产生的含人类绒毛膜促性腺激素抗原的研究 Ⅲ.β—Galactosidase—hCG 免疫和生物活性的初步探讨
短句来源
     STUDY ON THE hCG B- SUBUNIT C-TERMINAL 36 PEPTIDE CONTAINING HYBRID PROTEIN SYNTHESIZED IN λgtll hCG Y1089 1, ISOLATION AND CHARACTERIZATION OF B-GALA CTOSID ASE- hCG
     基因工程菌株产生人类绒毛膜促性腺激素抗原的研究——Ⅰ.β-Galactosidase-hCG的分离纯化与鉴定
短句来源
     High Resolution Two-dimensional Polyacrylamide Gel Electrophoresis (2D-PAGE) Analysis of Human Chorionie Gonadotropin(hCG)
     人类绒毛膜促性腺激素(HCG)的高分辨双向聚丙烯酰胺凝胶电泳分析
短句来源
     Objective To find out whether human chorionic gonadotrophin (hCG) could change the ability of invasion of trophoblast by regulating the production of cytokines.
     目的 揭示人类绒毛膜促性腺激素 (hCG)是否通过改变细胞因子的生成而影响滋养细胞的侵袭性。
短句来源
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  相似匹配句对
     The Human's Box
     人类的盒子
短句来源
     The Assignment of Human Genes
     人类基因定位
短句来源
     TOXICOLOGY AND SAFETY STUDIES OF HUMAN CHORIONIC GONADOTROPIN (hCG) CONTRACEPTIVE VACCINE ON RHESUS MONKEY
     人类绒毛膜促性腺激素避孕疫苗的毒理学和免疫安全性研究
短句来源
     High Resolution Two-dimensional Polyacrylamide Gel Electrophoresis (2D-PAGE) Analysis of Human Chorionie Gonadotropin(hCG)
     人类绒毛膜促性腺激素(HCG)的高分辨双向聚丙烯酰胺凝胶电泳分析
短句来源
     STUDIES ON A RECEPTOR RADIOASSAY SYSTEM FOR CHORIONIC GONADOTROPIN RECEPTORS
     绒毛膜促性腺激素受体的放射分析
短句来源
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  human chorionic gonadotropin
The gonads were incubatedin vitro without or with human chorionic gonadotropin.
      
The testicular content and baselinein vitro production of testosterone were not affected by prenatal stress, but the testicular response to human chorionic gonadotropin was higher in the stressed group.
      
The testosterone production was estimated under control conditions and under stimulation with human chorionic gonadotropin (CG).
      
Regulation of human chorionic gonadotropin secretion and messenger ribonucleic acid levels by follistatin in the NUCC-3 chorioca
      
No effect of human chorionic gonadotropin treatment due to threatened abortion in early pregnancy for birth outcomes
      
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The present investigation has been earried out to study hCG B-subunit C-terminal containing hybrind protein synthesized by λgt11 hCG Y1089 through genetic engineering technology. A 36 peptide gene containing h CG B-subunit C-terminal has been artificially synthesized by Oligonucleotide Synthesizer, the double stranded DNA was cloned into an expression vetor λgt11 and the 36 peptide gene fusion product was successfully expressed in host strain E. coli Y1089. h CG B-subunit C-terminal 36 peptide containing hybrid...

The present investigation has been earried out to study hCG B-subunit C-terminal containing hybrind protein synthesized by λgt11 hCG Y1089 through genetic engineering technology. A 36 peptide gene containing h CG B-subunit C-terminal has been artificially synthesized by Oligonucleotide Synthesizer, the double stranded DNA was cloned into an expression vetor λgt11 and the 36 peptide gene fusion product was successfully expressed in host strain E. coli Y1089. h CG B-subunit C-terminal 36 peptide containing hybrid protein (B-galactosidase-h CG) expressed in λgt11 hCG Y1089 was precipitated in the ammonium sulfate solution of 50% saturation, affinity isolated by anti-B-galactosidase sepharose 4B column and finally purified with preparative polyacrylamide gel electrophoresis. Comparative analysis of different preparations revealed that the eluate of affinity column gave a mjor band and two minor bands, and the eluate of preparative polyacrylamide gel electrophoresis only gave one band while the solution of ammonium sulfate precipitated gave more than 30 bands in polyacrylamide gel electrophoresis, it indicated that anti-B-galactosidase affinity chromatography and preparative polyacrylamide gel eleetrophoresis can be used for isolating and purifing B-galactosidase-h CG. The results of western blot technique demonstrated that B-galactosidase-hCG contains immunological property of hCG B-subunit C-terminal. Preliminary studies of antigenicitY of this B-galactosidase-hCG through counter current immunoelectrophoresis and double immunodiffusion techniques have clearly demonstrated that this Purified B-galactosidase-hCG can be crossly immunopreciPitated with rabbit anti-B-galactosidase-hCG serum, it has been concluded that B-galactosidase-hCG possesses the antigenieitY, but the biological activity of this this hybrid protein to neutralizing hCG has to be determined by in vitro or in vivo system.

通过寡核苷酸合成仪,用化学方法合成相当于hcGβ链C末端36肽基因的片段,成功地克隆到噬菌体载体λgt11中,并在宿主菌株E. coli y_(1089)中得到表达。含λgt11 hCG重组体的E. coli y_(1089)所合成的含人类绒毛膜促性腺激素β链c末36肽的杂交蛋白(下称:β—galactosidase—hCG),通过硫酸铵沉淀、亲和层析和制备性聚丙烯酰胺凝胶电泳,可得电泳纯单一色带的β—gaIactosidase—hCG。蛋白质转印(Western Blot)技术证实β—galactasidase—hCG具有hCGβ链c末端36肽的免疫特性。对流免疫电泳和免疫双扩散试验结果表明β—galactosidase—hCG具免疫原性。从一立升培养液的菌体中可分离纯化约3.8毫克的β—galaetosidase—hCG。

Hybridization of a cloned α-S-labelled cDNA probe homologous to the human chorionic gonadotropin β subunit (3HCG) to the spots of total DNA from liver tissue of grass carp (Ctenopharynogodon idellus) on the nitrocellular filter shows there is the homologue to βHCG gene in fish genome.This gene homologue was assigned to terminal portion on an arm of a m-group chromosome of grass carp by in situ hybridization.The significance of finding and mapping the βHCG gene homologue was discussed.

本研究以~(?)s标记的人类绒毛膜促性腺激素(humgn chorionic gonadotropin)β链(βHCG)的克隆基因(cDNA)为探针,分別与人类、草鱼(Cteaopharynogodon idellus)和枯草芽胞杆菌(Bacillus subtilis)的总DNA进行点杂交,以~3H标记的同一探针与草鱼中期染色体作原位杂交,结果表明:草鱼基因组中存在βHCG基因的同类物,这一同类物的基因座位在草鱼m组一染色体(可能是第4号染色体)一个臂的末端。本文对所得结果的意义进行了讨论。

An artificial gene equivalent to 36 C-terminal peptide of human chorionic gonadotropin (hCG) β-subunit has been chemically synthesized through the application oligonucleotide synthesizer and the double strand DNA has heen successfully cloned into an expression vector, λgtll, and transfected into the host strain E.Coli Y1089, through recombinant phage λgtll hCG to produce a hybrid protein containing hCG-β-suhunit 36 C-terminal peptide (Called β-galactosidase-hCG). Anti-serum of rat immunized with β-galactosidase-hCG...

An artificial gene equivalent to 36 C-terminal peptide of human chorionic gonadotropin (hCG) β-subunit has been chemically synthesized through the application oligonucleotide synthesizer and the double strand DNA has heen successfully cloned into an expression vector, λgtll, and transfected into the host strain E.Coli Y1089, through recombinant phage λgtll hCG to produce a hybrid protein containing hCG-β-suhunit 36 C-terminal peptide (Called β-galactosidase-hCG). Anti-serum of rat immunized with β-galactosidase-hCG was detected by counter current electrophoresis and immunodiffusion, the results hare clearly demonstrated that β-galactosidase hCG can be crossly immunoprecipitated with rat anti-β-galactosidase-hCG serum, whereas using hCG replaced β-galactosidase-hCG, the anti-serum produced against β-galactosidase-hCG has no cross reaction with hCG. When β-galactosidase-hCG was treated with 2M Urea, 0.9M SDS and 0.005M Mercaptoethanol solvent system before using as an antigen to immunize rat, the anti-body against this depolymerized antigen can be crossly immunoprecipitated with hCG. It revealed that the β-galactosidase-hCG polymer was depolymerized and the conformation of β-galactosidase-hCG subunit has been changed, therefore the antigenic determinant of hCG-β-subunit 36C-terminal peptide in β-galactosidase-hCG appeared its immunogenicity in Urea and SDS solvent. the depolymerized optimal condictions for, β-galaztosidase-hCG in Urea and SDS solvent system were 60C in 40 minutes.

基因工程菌株产生的含人类绒毛膜促性腺激素(hCG)β亚单位C末端36肽的β-半乳糖苷酶杂交蛋白(简称β-galaclosidase-hCG),在未经解聚处理前免疫新西兰种白兔和昆明种小白鼠,其抗血清在免疫双扩散试验和对流免疫电泳中对hCG没有形成沉淀线作用。而此杂交蛋白经解聚处理后,其抗血清与hCG相作用,在对流免疫电泳和免疫双扩散试验中都出现抗原抗体反应的免疫沉淀线。从多种蛋白质解聚系统中,经过反复试验,筛选出对β-galactosidase-hCG杂交蛋白有良好解聚作用的解聚剂为2M脲、0.9MSDS、0.005M巯基乙醇;解聚作用最适温度是60℃,最适时间为40分钟。

 
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