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   人类绒毛膜促性腺激素 在 生物学 分类中 的翻译结果: 查询用时:0.098秒
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  human chorionic gonadotropin
    An artificial gene equivalent to 36 C-terminal peptide of human chorionic gonadotropin (hCG) β-subunit has been chemically synthesized through the application oligonucleotide synthesizer and the double strand DNA has heen successfully cloned into an expression vector, λgtll, and transfected into the host strain E. Coli Y1089, through recombinant phage λgtll hCG to produce a hybrid protein containing hCG-β-suhunit 36 C-terminal peptide (Called β-galactosidase-hCG).
    基因工程菌株产生的含人类绒毛膜促性腺激素(hCG)β亚单位C末端36肽的β-半乳糖苷酶杂交蛋白(简称β-galaclosidase-hCG),在未经解聚处理前免疫新西兰种白兔和昆明种小白鼠,其抗血清在免疫双扩散试验和对流免疫电泳中对hCG没有形成沉淀线作用。
短句来源
    Human Chorionic Gonadotropin(hCG)β-subunit C-terminal peptide containing hybrid protein(β-galactosidase-hCG)produced by the recombinant λgt 11 hCG in host strain E. coli Y1089 was isolated and purified by ammoniun sulfate precipitated,anti-β-galactosidase affinity column. Pooled sera from various experimental groups were tested for anti-β-hCG activity in assays of in vivo neutralizing activity.
    基因工程菌株产生的含人类绒毛膜促性腺激素(hCG)β亚单位 C 末端36肽的β-半乳糖苷酶杂交蛋白(简称:β-Galactosidasc-hCG),主动免疫昆明种小白鼠,能诱发特异抗 hCG 抗体的产生。
短句来源
  human chorionic gonadotropin
    An artificial gene equivalent to 36 C-terminal peptide of human chorionic gonadotropin (hCG) β-subunit has been chemically synthesized through the application oligonucleotide synthesizer and the double strand DNA has heen successfully cloned into an expression vector, λgtll, and transfected into the host strain E. Coli Y1089, through recombinant phage λgtll hCG to produce a hybrid protein containing hCG-β-suhunit 36 C-terminal peptide (Called β-galactosidase-hCG).
    基因工程菌株产生的含人类绒毛膜促性腺激素(hCG)β亚单位C末端36肽的β-半乳糖苷酶杂交蛋白(简称β-galaclosidase-hCG),在未经解聚处理前免疫新西兰种白兔和昆明种小白鼠,其抗血清在免疫双扩散试验和对流免疫电泳中对hCG没有形成沉淀线作用。
短句来源
    Human Chorionic Gonadotropin(hCG)β-subunit C-terminal peptide containing hybrid protein(β-galactosidase-hCG)produced by the recombinant λgt 11 hCG in host strain E. coli Y1089 was isolated and purified by ammoniun sulfate precipitated,anti-β-galactosidase affinity column. Pooled sera from various experimental groups were tested for anti-β-hCG activity in assays of in vivo neutralizing activity.
    基因工程菌株产生的含人类绒毛膜促性腺激素(hCG)β亚单位 C 末端36肽的β-半乳糖苷酶杂交蛋白(简称:β-Galactosidasc-hCG),主动免疫昆明种小白鼠,能诱发特异抗 hCG 抗体的产生。
短句来源
  “人类绒毛膜促性腺激素”译为未确定词的双语例句
    Study on the Immunogenicity of β-galactosidase-hCG Polymer Containing hCG-β-subunit C-terminal Peptide Synthesized in λgtll hCG Y1089
    基因工程菌株产生含人类绒毛膜促性腺激素抗原的研究 Ⅱ.β—galactosidase—hCG聚体蛋白免疫原性
短句来源
    Study on the immunological and Biological Activity Asay of β-Galactosidase-hCG containing hCG β-subunit C-terminal Peptitle Synthesized in λgt 11 hCG Y1089
    基因工程菌株产生的含人类绒毛膜促性腺激素抗原的研究 Ⅲ.β—Galactosidase—hCG 免疫和生物活性的初步探讨
短句来源
    High Resolution Two-dimensional Polyacrylamide Gel Electrophoresis (2D-PAGE) Analysis of Human Chorionie Gonadotropin(hCG)
    人类绒毛膜促性腺激素(HCG)的高分辨双向聚丙烯酰胺凝胶电泳分析
短句来源
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Hybridization of a cloned α-S-labelled cDNA probe homologous to the human chorionic gonadotropin β subunit (3HCG) to the spots of total DNA from liver tissue of grass carp (Ctenopharynogodon idellus) on the nitrocellular filter shows there is the homologue to βHCG gene in fish genome.This gene homologue was assigned to terminal portion on an arm of a m-group chromosome of grass carp by in situ hybridization.The significance of finding and mapping the βHCG gene homologue was discussed.

本研究以~(?)s标记的人类绒毛膜促性腺激素(humgn chorionic gonadotropin)β链(βHCG)的克隆基因(cDNA)为探针,分別与人类、草鱼(Cteaopharynogodon idellus)和枯草芽胞杆菌(Bacillus subtilis)的总DNA进行点杂交,以~3H标记的同一探针与草鱼中期染色体作原位杂交,结果表明:草鱼基因组中存在βHCG基因的同类物,这一同类物的基因座位在草鱼m组一染色体(可能是第4号染色体)一个臂的末端。本文对所得结果的意义进行了讨论。

An artificial gene equivalent to 36 C-terminal peptide of human chorionic gonadotropin (hCG) β-subunit has been chemically synthesized through the application oligonucleotide synthesizer and the double strand DNA has heen successfully cloned into an expression vector, λgtll, and transfected into the host strain E.Coli Y1089, through recombinant phage λgtll hCG to produce a hybrid protein containing hCG-β-suhunit 36 C-terminal peptide (Called β-galactosidase-hCG). Anti-serum of rat immunized with β-galactosidase-hCG...

An artificial gene equivalent to 36 C-terminal peptide of human chorionic gonadotropin (hCG) β-subunit has been chemically synthesized through the application oligonucleotide synthesizer and the double strand DNA has heen successfully cloned into an expression vector, λgtll, and transfected into the host strain E.Coli Y1089, through recombinant phage λgtll hCG to produce a hybrid protein containing hCG-β-suhunit 36 C-terminal peptide (Called β-galactosidase-hCG). Anti-serum of rat immunized with β-galactosidase-hCG was detected by counter current electrophoresis and immunodiffusion, the results hare clearly demonstrated that β-galactosidase hCG can be crossly immunoprecipitated with rat anti-β-galactosidase-hCG serum, whereas using hCG replaced β-galactosidase-hCG, the anti-serum produced against β-galactosidase-hCG has no cross reaction with hCG. When β-galactosidase-hCG was treated with 2M Urea, 0.9M SDS and 0.005M Mercaptoethanol solvent system before using as an antigen to immunize rat, the anti-body against this depolymerized antigen can be crossly immunoprecipitated with hCG. It revealed that the β-galactosidase-hCG polymer was depolymerized and the conformation of β-galactosidase-hCG subunit has been changed, therefore the antigenic determinant of hCG-β-subunit 36C-terminal peptide in β-galactosidase-hCG appeared its immunogenicity in Urea and SDS solvent. the depolymerized optimal condictions for, β-galaztosidase-hCG in Urea and SDS solvent system were 60C in 40 minutes.

基因工程菌株产生的含人类绒毛膜促性腺激素(hCG)β亚单位C末端36肽的β-半乳糖苷酶杂交蛋白(简称β-galaclosidase-hCG),在未经解聚处理前免疫新西兰种白兔和昆明种小白鼠,其抗血清在免疫双扩散试验和对流免疫电泳中对hCG没有形成沉淀线作用。而此杂交蛋白经解聚处理后,其抗血清与hCG相作用,在对流免疫电泳和免疫双扩散试验中都出现抗原抗体反应的免疫沉淀线。从多种蛋白质解聚系统中,经过反复试验,筛选出对β-galactosidase-hCG杂交蛋白有良好解聚作用的解聚剂为2M脲、0.9MSDS、0.005M巯基乙醇;解聚作用最适温度是60℃,最适时间为40分钟。

hCG was analysed with high resolution 2D—PAGE. This glycoprotin was first separated by isoelectric focusing (IEF), and then the analysis of hCG was Carried out in second dimension by using gradient sodium dodecyl sulfate polyacrylmaide gel electrophoresis (SDS—PAGE). After electrophoresis the slat gel was treated with silver stain. Three protein bands appeared clearly on the gel, designated as A,B and C bands, On the basis of determinations of PH values and molecular weights. Band A, with molecular waight egual...

hCG was analysed with high resolution 2D—PAGE. This glycoprotin was first separated by isoelectric focusing (IEF), and then the analysis of hCG was Carried out in second dimension by using gradient sodium dodecyl sulfate polyacrylmaide gel electrophoresis (SDS—PAGE). After electrophoresis the slat gel was treated with silver stain. Three protein bands appeared clearly on the gel, designated as A,B and C bands, On the basis of determinations of PH values and molecular weights. Band A, with molecular waight egual to 36k, is the protein band of the uncleared hCG. Band B, with 23k, is that of the β-Subunit of hCG, while band C, with 17k, the α-subunit of hCG 2D-PAGE with silver stain provide valuable informations for the structural and characteristic analysis of subunit fragments and the whole molecule of hCG.

采用高分辨的双向聚丙烯酰胺凝胶电泳(2D—PAGE)对人类绒毛膜促性腺激素(~bCG)进行分析。第一向等电点聚焦电泳(IEF)后,在第二向SDS—聚丙烯酰胺梯度凝胶垂直板上电泳(SDS)—PAGE),电泳结束,用银染色法染色,电泳图谱上得到三条主要的蛋白区带,即A,B和C带,并测定了它们的pH值和分子量,A带分子量是36K为未裂解的~hCG,B带分子量是23K为~hCG的β—基亚,C带分子量是17K为~hCG的α—亚基,通过双向电泳的分析和鉴定,为~hCG的分子结构在双向电泳中的特性提供了初步参考数据。

 
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