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培养肝细胞
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  cultured hepatocyte
     Results pIRES-EYFP/VEGF121 plasmid was constructed suc-cessfully and transfected into rat primary cultured hepatocyte.
     【结果】 pIRES-EYFP/VEGF121得以成功构建,并转染大鼠原代培养肝细胞;
短句来源
     Methods Recombinant plasmid pIRES- EYFP/VEGF121 was transfected into rat primary cultured hepatocyte. The transfection and expres-sion of which was rapidly examined by using flow cytometer (FCM) with enhanced yellow fluoresent protein (EYFP) as a marker. The condition of pIRES-EYFP/VEGF121 transfection and expression were optimized on the based results.
     【方法】 以加强型黄色荧光素蛋白(EYFP)为标记,用FCM快速检测重组质粒pIRES-EYFP/VEGF121在大鼠原代培养肝细胞中转染表达,根据结果优化pIRES-EYFP/VEGF121转染表达条件?
短句来源
     Conclusion Exogenous VEGF genes transfection and expression in rat prima-ry cultured hepatocyte can be examined and optimized conveniently and quickly by FCM with EYFP as a marker. Based on the results, the condition of transfection and expression can be optimized. The back-ground for rat primary cultured hepatocyte transplantation and liver-derived gene therapy would be estabil-shed.
     【结论】以EYFP为标记, FCM可简便快捷地检测并优化外源性VEGF基因在大鼠原代培养肝细胞转染表达,可为研究VEGF基因修饰大鼠原代培养肝细胞移植和肝基因治疗打基础?
短句来源
     Rapid Optimization of Gene Transfection in Rat Primary Cultured Hepatocyte by Using Flow Cytometry
     流式细胞仪快速优化大鼠原代培养肝细胞基因转染
短句来源
     Objective:To understand the important effect of the liver on the metabolism of lipid from the hyperlipemia model of rabbit and the influence of lipoprotein receptor in the cultured hepatocyte membrane of the tiaogandaozhou decoction,observe the action of preventing the formation and progress of atherosclerosis of it.
     目的 :观察中药调肝导浊汤对家兔实验性AS模型肝脏脂代谢及体外培养肝细胞膜脂蛋白受体的影响 ,探讨中药防治AS的机制。
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  culture hepatocyte
     Effect of transforming growth factor-β on the primary culture hepatocyte and liver cancer cell BEL7401
     TGF-β对原代培养肝细胞及肝癌细胞BEL7401生长作用的影响
短句来源
     Objective To rapidly examine exogeous VEGF genes transfection and expression in rat primary culture hepatocyte by using flow cytometry, and optimize the condition of exogeoukxs VEGF genes transfection and expression according to the results.
     【目的】 用流式细胞仪(FCM)快速检测外源性血管内皮生长因子基因(VEGF基因)在大鼠原代培养肝细胞转染表达,根据结果优化其转染表达条件?
短句来源
     Clustering Analysis of Gene Express Profiles of Mouse Primary Culture Hepatocyte Induced by 13 Chemicals
     十三种化合物诱导的小鼠原代培养肝细胞基因表达谱的聚类分析
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  “培养肝细胞”译为未确定词的双语例句
     Dexamethasone could partly inhibit the ICAM 1 expression on human hepatocytes induced by TNF α,IL 1 and IFN γ. The inhibition rates were 40.3%±5.9%,38.1%±4.8%, and 37.6%±6.7%, respectively.
     地塞米松能部分抑制TNF α、IL 1、IFN γ所诱导的人原代培养肝细胞ICAM 1增强表达 ,其抑制率分别为 (4 0 .3± 5 .9) %、(3 8.1± 4.8) %和 (3 7.6± 6.7) %。
短句来源
     Objective To study the effects of IFN γ,TNF α,IL 1 and dexamethasone on the expression of intercellular adhesion molecule 1(ICAM 1) of primary cultured human hepatocyte.
     目的 研究IFN γ、TNF α、IL 1和地塞米松对人原代培养肝细胞细胞间黏附分子 1(ICAM 1)表达的影响。
短句来源
     Results With the increment of the release of TNFα, IL-6 and NO, GOT and GPT were increased in hepatocytes while ALB was decreased.
     结果 KC上清中TNFα、IL - 6和NO的浓度增高的同时 ,培养肝细胞GOT和GPT增高 ,同时ALB降低。
短句来源
     Methods ICAM 1 expression of primary cultured human hepatocyte was induced in vitro by IFN γ,TNF α and IL 1, measuring by cellular enzyme linked immunosorbent assay(ELISA).
     方法 用IFN γ、TNF α和IL 1在体外诱导人原代培养肝细胞表达ICAM 1,并用细胞ELISA检测其ICAM 1表达水平 ;
短句来源
     Conclusions Enhanced ICAM 1 expression of primary cultured human hepatocyte may be induced in vitro by IFN γ,TNF α and IL 1, which may be partly inhibited by dexamethasone.
     结论 IFN γ、TNF α和IL 1能诱导人原代培养肝细胞ICAM 1增强表达 ,而地塞米松能抑制其表达。
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  cultured hepatocyte
Further studies are required to determine whether KRG also might stimulate cultured hepatocyte in vitro.
      
Measurement of DFH fluorescence can be used as a rapid readout of CYP3A activity following microsomal or cultured hepatocyte incubations.
      
The data obtained in cultured hepatocyte couplets in this study show that the redistribution of mrp2 to the apical domain takes place in three steps.
      


Rat liver cells were isolated by enzyme perfusion. The Dissociated cells were mcubated at 37℃ in the presence of 5% CO_2. Four hours later, they were incubated with aflatoxin B_1 for 15 minutes to 6 hours. Cells incubated in a medium without Aflatoxin B_1 were used as tne control. Both aflatoxin B_1 treated and untreated cells were fixed in situ with 1.6% glutaraldehyde. They were detached from their substrate and centrifuged. The pellets were dehydrated in ethanol and embedded in Epon 812. The ribonucleo-protein...

Rat liver cells were isolated by enzyme perfusion. The Dissociated cells were mcubated at 37℃ in the presence of 5% CO_2. Four hours later, they were incubated with aflatoxin B_1 for 15 minutes to 6 hours. Cells incubated in a medium without Aflatoxin B_1 were used as tne control. Both aflatoxin B_1 treated and untreated cells were fixed in situ with 1.6% glutaraldehyde. They were detached from their substrate and centrifuged. The pellets were dehydrated in ethanol and embedded in Epon 812. The ribonucleo-protein were preferentially stained by the uranyl-EDTA-lead regressive method.

黄曲霉毒素B_1是致癌因素之一。本文用离体培养肝细胞,观察黄曲霉素B_1对肝细胞间期核超微结构及RNA转录、加工的影响,并探讨其作用机理。

In this paper, low concentration and equal quantum of collagenase and trypsin were mixed and used to digest directly small piece of liver tissue of baby rats. Large amount of vital cells were obtained by using this method. Cultured hepatocytes are epithelioid cells and have various shape. The cells can be classified large ones and small ones. In the cytoplasm, large amount of granulations were observed, were observed. With histochemical observation, many glucogen granulation can be demonstrated. In hepatocytes,...

In this paper, low concentration and equal quantum of collagenase and trypsin were mixed and used to digest directly small piece of liver tissue of baby rats. Large amount of vital cells were obtained by using this method. Cultured hepatocytes are epithelioid cells and have various shape. The cells can be classified large ones and small ones. In the cytoplasm, large amount of granulations were observed, were observed. With histochemical observation, many glucogen granulation can be demonstrated. In hepatocytes, the activity of SDH and LDH is high. Cultured hepatocytes have the function of hepatocytes in whole liver and can be used as experimental mould in studying physiological function,differentiation and cancer research of hepatocyte.

本文用等量混合低浓度胶原酶、胰蛋白酶,直接消化乳鼠肝组织小块的方法,获得了存活率较高的大量肝细胞。培养的乳鼠肝细胞呈上皮状,形态多样,可分为大形细胞和小形细胞,胞质内充满颗粒。在培养的肝细胞内糖原丰富,琥珀酸脱氢酶活性强,乳酸脱氢酶活性阳性。培养的乳鼠肝细胞具有整体肝中细胞的特殊功能,可做为研究肝细胞的实验模型。

Protective effect of ATP-magnesium chloride to hepatocytes lacking oxygen and glucose was investigated by histochemical and ultrastructural methods. Stock culture of Wistar rat hepatocytes was carried out for 4 to 6 days. The hepatocytes in the forty culture flasks that grew well were divided into: normal group, group lacking oxgen and glucose added with ATP-magnesium chloride, glucose control group and enzyme control group. All groups were cultured for sixty minutes at 37℃. PAS reactions were operated and the...

Protective effect of ATP-magnesium chloride to hepatocytes lacking oxygen and glucose was investigated by histochemical and ultrastructural methods. Stock culture of Wistar rat hepatocytes was carried out for 4 to 6 days. The hepatocytes in the forty culture flasks that grew well were divided into: normal group, group lacking oxgen and glucose added with ATP-magnesium chloride, glucose control group and enzyme control group. All groups were cultured for sixty minutes at 37℃. PAS reactions were operated and the activiy of SDH, LDH and ACP was tested on coverglasses. The hepatocytes in ninety culture flasks that grow well were also divided into normal group, the group lacking oxygen and glucose, and the group added With ATP-magnesium chloride. The result showed that PAS reaetion of the group added with ATP-magnesium chloride is strong positive, the activity of SDH was greatly enhanced; activity of LDH and ACP was greatly reduced. After adding ATP-magnesium chloride the damaged mitochondria, endoplastic reticula and microvilli were restored to varying degree. In a word, ATP-magnesium chloride protects effectively the hepatocytes lacking oxygen and glucose from damage.

本文从组织化学及超微结构角度探讨了ATP-MgCl_2对缺氧缺糖培养肝细胞的保护作用。将Wistar系大白鼠乳鼠肝组织经冷消化制成细胞悬液,原代单层培养4~6天,取生长良好的培养瓶共40瓶,将其分为4组:正常组,缺氧缺糖组,缺氧缺糖条件下加药组,糖原对照组及酶的对照组,4组都在37℃恒温培养箱里培养60min,取出盖玻片进行PAS反应并显示SDH,LDH及ACP活性,同时取正常组、缺氧缺糖组、加药组肝细胞30瓶,进行透射电镜观察,结果加药组PAS反应呈强阳性,SDH活性增强,LDH,ACP活性减弱。电镜下可见加药后损伤的线粒体、内质网、微绒毛得以恢复正常,糖原含量增加,本项工作证明ATP-MgCl_2对缺氧缺糖的培养肝细胞具有良好的保护作用。

 
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