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斑点杂交法     
相关语句
  spot hybridization
     Experimental study Detecting Entroviruses RNA With the Method of cDNA: RNA Spot Hybridization
     用cDNA:RNA斑点杂交法检测肠道病毒RNA的实验研究
短句来源
     METHODS:Serum HBV markers and HBV-DNA were determined by ELISA and direct spot hybridization in 95 cases of NHL.
     方法 :对 95例中高度NHL患者 ,采用ELISA法和直接斑点杂交法检测血清中的HBV标志物及DNA ,常规方法检测化疗前后肝功能。
短句来源
     The serum, semen, saliva and urine in patients infected with HBV were assayed for HBV-DNA by direct spot hybridization technique and for DNA-P(RIA technique).
     本文采用斑点杂交法和放射免疫法检测乙肝患者血液、唾液、尿液以及精液中的HBV-DNA和DNA-P。
短句来源
     Detection of HBV DNA in Serum with Spot Hybridization
     应用斑点杂交法测定血清中乙型肝炎病毒的DNA
短句来源
     By using EIA and Nucleic Acid Spot Hybridization, we examined HDAg, Anti-HD, and HDV-RNA among 227 patients with hepatitis B in some areas of Fujian province.
     本研究应用酶联免疫(EIA)及核酸斑点杂交法对我省部分地区227例乙型肝炎患者检测了HDAg、抗-HD及HDVRNA。
短句来源
更多       
  blot hybridization probing technic
     Methods Comparison was made between the solid phase of radio immunoassay in 9 kinds of various markers for HBV serum(HBV-M) and 32 P HBV-DNA blot hybridization probing technic for HBV-DNA examination.
     方法 用固相放射免疫法检测各类乙型肝炎血清乙肝病毒九项标志物 ,与斑点杂交法3 2 PHBV -DNA探针检测血清HBV -DNA对照。
短句来源
  dot blot hybridization method
     Detection of rpoB mutations in rifampin resistant Mycobacterium tuberculosis strains by the reverse dot blot hybridization method
     应用反向斑点杂交法检测耐利福平结核分支杆菌rpoB基因突变
短句来源
     Dot blot hybridization method was used to determine the level of mRNA of procollagens in lungs of asbestosis rats, at 30 and 60 days after instillation of asbestoes. The results indicated that amount of 3 kinds of procollagen mRNAs were all higher than that in normal rat lung.
     以斑点杂交法测定染溫石棉尘后30天与60天大鼠肺组织中前胶原mRNA的水平,即proα_1(Ⅰ)、proα_2(Ⅰ)、proα_1(Ⅲ)mRNA的水平,并与正常大鼠肺组织对比,结果显示,染石棉尘的肺组织中这三种mRNA显著地高于正常对照组。
短句来源
     More than 1 000 shrimp samples carrying white spot syndrome virus(WSSV) from Rushan shrimp farm were detected by using PCR-DNA probe dot blot hybridization method.
     2002年采用PCR-核酸探针斑点杂交法检测了乳山对虾养殖场1000余份样品携带白斑综合征病毒(WSSV)的情况。
短句来源
  dot blot method
     The content of TGFβ1,, type Ⅰ and Ⅲ collagen proteins and the expression of TGFβ1,, α1, (Ⅰ) and a, (Ⅲ) procollagen mRNA in the different stage of hepatic fibrogenesis was detected by immunohistochemical staining and Dot blot method, respectively.
     分别用免疫组化法和斑点杂交法检测肝纤维化不同阶段TGFβ1、Ⅰ、Ⅲ型胶原蛋白及其mRNA表达。
短句来源
     Absorbance of digoxigenin-labelled probe was higher than that of biotin-labelled Probe for detectingCSV c DNA with PCR-microplate hybridization. The sensitivity of the digoxigenin-fataled probe for detecting the CSV RNA by dot blot method was 50fg, while that of the biotin-labelled Probe for detectingthe CSV RNA was 5pg.
     采用PCR-微量板杂交法,地高辛标记的探针检测CSVcDNA的吸光值明显高于生物素标记的探针检测的吸光值,采用斑点杂交法,地高辛标记的cDNAN针能够检测CSVRNA的最低含量为50fg,而生物素标记的cDNA探针能够检测CSVRNA的最低含量为5pg。
短句来源

 

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      dot blot technique
    The specificity of perch (Perca fluviatilis) roe fucolectin was studied using the protein dot blot technique, followed by detection with colloidal gold-labeled neoglycoproteins bearing human milk oligosaccharides.
          
    A dot blot technique for the analysis of interactions of lectins with glycosaminoglycans
          
    A dot blot technique is presented by which the interaction of lectins with glycosaminoglycans can be analysed.
          
    This type of test is similar to the reverse dot blot technique and has been developed by Roche Molecular Systems.
          
    The results obtained by studying different Brucella strains and biovars by the dot blot technique are provided in Table 1.
          
      spot hybridization
    Of these sera, 171 were available for testing for hepatitis B virus DNA as a marker of infectivity by spot hybridization.
          
    After having been made radioactive at the 5'OH terminus, the RNA fragments were used as probes in a spot hybridization experiment.
          
    Variance analysis demonstrated that the spot hybridization signal intensity was similar for 3,954 ESTs (85.8%) after 6?h of bacterial growth.
          
    Detection of potato spindle tuber viroid by nucleic acid spot hybridization: Evaluation with tuber sprouts and true potato seed
          
    The spot hybridization test detected PSTV in mixtures of seed extracts equivalent to as few as one seed from a PSTV-infected plant and 80 seeds from healthy plants.
          
    更多          
      dot blot hybridization method
    Ki-ras PM was assessed by the allele-specific oligonucleotide dot blot hybridization method, and the expression of p21 andp53 was assessed by an immunohistochemical staining method with monoclonal antibodies.
          
    The colony hybridization technique is more easily and quickly applied than the dot blot hybridization method.
          
      其他


    Epidemiologic data indicate that a large proportion of human population has been exposed to hepatitis B virus (HBV) and infection is of common occurrence. According to the molecular gen-tic theory, hepatitis B infection is due to HBV DNA. So, it is important to establish a convenient laboratory method for the detection of HBV in serum.HBV particles in serum by spot hybridization technique, Dane particles are pelleted by centri-fugation, treated with alkaline, the applied to the nitrocellulose filters. The filters...

    Epidemiologic data indicate that a large proportion of human population has been exposed to hepatitis B virus (HBV) and infection is of common occurrence. According to the molecular gen-tic theory, hepatitis B infection is due to HBV DNA. So, it is important to establish a convenient laboratory method for the detection of HBV in serum.HBV particles in serum by spot hybridization technique, Dane particles are pelleted by centri-fugation, treated with alkaline, the applied to the nitrocellulose filters. The filters were neutralized and baked utder vacuum. HBV DNA is detected by its ability to hybridize with 32P-labe-lled DNA prepared by nick translation technique from recombinant NC-1 plasmids which contains the complete HBV genome. After hybridization, the nitrocellulose membrane is washed and autora-diographed on X-ray film. This method is very sensitive and even 0.5pg of HBV DNA can be detected.Eighty-five different titer-positive HBsAg sera were detected by this method. More than 70% were HBV DNA positive. The level of HBV DNA did not quantitatively correspond to the HBsAg level. There were 7 HBV DNA positive in 17 sera with titers of HBsAg less than 1:8. This result impli d that some HBsAg negative sera still can transmitte hepatitis.Twenty-three high titer of HBsAg sera, all being DNA polymerase positive, were examined. All of them were HBV DNA positive.Two sera of DNA polymerase negative were HBV DNA negative. Thus, the endogenous DNA polymerase activity approximately corresponds to the amount of HBV DNA and titer of infectious HBV.

    应用斑点杂交法探讨血清中HBV-DNA水平与HBV表面抗原以及内源性DNA多聚酶的关系,结果表明:DNA多聚酶水平可反应血清中DNA水平,HBsAg滴度与HBV-DNA相关,但HBV-DNA的含量与HBsAg滴度并不成正比。

    The conditions for detecting tobacco mosaic virus (TMV) RNA in tobacco leave extract by RNA dot hybridization using a radioactive probe containing the cDNA of 3' terminal RNA of TMV strain OM have been studied. These conditions include the homology between the TMV strains isolated from Yunnan and Shanghai and the strain OM, the method for extracting viral RNA from tobacco leave and for adsorbing the extracted viral RNA onto nitrocellulose filter efficiently, the material interrupting the adsorption of viral...

    The conditions for detecting tobacco mosaic virus (TMV) RNA in tobacco leave extract by RNA dot hybridization using a radioactive probe containing the cDNA of 3' terminal RNA of TMV strain OM have been studied. These conditions include the homology between the TMV strains isolated from Yunnan and Shanghai and the strain OM, the method for extracting viral RNA from tobacco leave and for adsorbing the extracted viral RNA onto nitrocellulose filter efficiently, the material interrupting the adsorption of viral RNA onto nitrocellulose filter and the hybridization of viral RNA with probe, and the speciality, sensitivity of the RNA dot hybridization method for detecting TMV-RNA in tobacco leave.

    用普通烟草花叶病毒OM株3′-端约2kb的cDNA为探针,探索了用RNA斑点杂交法对烟草组织中烟草花叶病毒RNA进行检测的条件。这些条件包括用分子杂交法观察云南烟区和上海烟草上分到的烟草花叶病毒与OM株的同源性,从烟草组织中提取烟草花叶病毒的几种方法的比较,使RNA有效地固定在硝酸纤维素滤膜上的方法,烟草组织中是否有干扰RNA固定和杂交的物质,斑点杂交方法检测烟草花叶病毒的特异性、灵敏度等。

    The serum, semen, saliva and urine in patients infected with HBV were assayed for HBV-DNA by direct spot hybridization technique and for DNA-P(RIA technique). It was found that the serum HBV-DNA would always be postive if re-rum DNA-P has been positive and it would also be positive, whenever the semen, saliva or urine HBV-DNA was positive. When the serum HBV-DNA was negative, HBV-DNA would not be detected in saliva or urine too. It was further found that the serum, semen, saliva or urine HBV-DNA could be detected...

    The serum, semen, saliva and urine in patients infected with HBV were assayed for HBV-DNA by direct spot hybridization technique and for DNA-P(RIA technique). It was found that the serum HBV-DNA would always be postive if re-rum DNA-P has been positive and it would also be positive, whenever the semen, saliva or urine HBV-DNA was positive. When the serum HBV-DNA was negative, HBV-DNA would not be detected in saliva or urine too. It was further found that the serum, semen, saliva or urine HBV-DNA could be detected not only in the serum HBeAg Positive but also in the HBe Ag negative patients. Conclusions- (1) The blood of HBV infected patients is the most infections as the positive rate of serum HBV-DNA and DNA-P are the highest. (2) Patients with negative HBeAg might also be infections. (3) Saliva might be important in the epidemiology of hepatitis B since in nearly half of the patients HBV-DNA has been detected in saliva. (4) Semen might serve as an infections agent to the patient's mate particularly might cause transmission of the disease to the child.

    本文采用斑点杂交法和放射免疫法检测乙肝患者血液、唾液、尿液以及精液中的HBV-DNA和DNA-P。血清中HBV-DNA和DNA-P的阳性检出率基本一致。精液、唾液和尿液中HBV-DNA阳性者,血清中HBV-DNA均阳性。血清中HBV-DNA阴性者,唾液、尿液中均未检出HBV-DNA。血清中HBeAg阳性与否,在血清、精液,唾液、尿液中均能检出HBV-DNA。文章认为:(1)血清中HBV—DNA、DNA-P的检出率最高,传染性较强。(2)血清中HBeAg阴性者,四种体液中亦可检出HBV-DNA,不能排除其传染性。(3)唾液中HBV—DNA的检出率为45.45%,其流行病学意义值得重视。(4)精液中检出HBV-DNA,父系垂直传播也是值得研究的一个问题。

     
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