Objective:To observe the effect of 5-aza-2'-deoxycytidine on proliferation and apoptosis of ovarian cancer cell lines(SKOV3 and 3AO)and on expression of mismatch repair(MMR)genes(hMLH1 and hMSH2)in SKOV3 and 3AO cells.
Four plasmids,namely,GSK-3βS9A,GID5-6,GID5- 6LP and the control vector,were cotransfected respectively with the green fluorescent protein (GFP) into SKOV3 cells by electroporation,and then BrdU incorporation assay was adopted to analyse the role of GSK- 3βactivity in the proliferation of ovarian cancer cells.
Adhesion induces matrix metalloproteinase-9 gene expression in ovarian cancer cells
Methods: Following adhesion of ovarian cancer cells A2780 to fibronectin, MMP mRNA expression was assayed by using reverse transcription-polymerase chain reaction (RT-PCR).
The effect of the MDM2-p53 loop on the sensitivity of ovarian cancer cells to cisplatin
It is concluded that cisplatin could induce the apoptosis of ovarian cancer cells, and the over-expression of Bcl-2 and Bcl-xl genes may contribute to apoptotic inhibition and the development of multidrug-resistance of human ovarian cancer.
Construction of antisense MT1-MMP vector and its inhibitory effects on invasion of human ovarian cancer cells
Objective: To study the gene expression of high metastatic human ovarian carcinoma cell line (HO-8910PM) and to screen for novel metastasis- associated genes by cDNA microarray.
Methods: The cDNA was retro-transcribed from equal quantity mRNA derived from tissues of highly metastatic ovarian carcinoma cell line and normal ovarian, and was labeled with Cy5 and Cy3 fluorescence as probes.
Establishment of intraperitoneal transplantation model of cisplatin-resistant ovarian carcinoma cell in scid mice
Inhibitory effects of anti-sense PTTG on malignant phenotype of human ovarian carcinoma cell line SK-OV-3
To construct cukaryotic expression vector expressing full length anti-sense pituitary tumor transforming gene (PTTG) mRNA and observe its blocking effect on the potential invasion of human ovarian carcinoma cell line SK-OV-3.
The Cx43 expression was detected by flowcytometry, Western, blot, and immunofluorescence in two ovarian tumor cell lines OVCAR3, CaOV3 before and after RA treatment.
Taken together, these observations suggest that GnRH-I and GnRH-II play key regulatory roles in ovarian tumor cell invasion and extracellular matrix degradation.
Furthermore, rTNF and OK-432 were cytostatic to most ovarian tumor cell lines examined.
Monoclonal antibodies against an ovarian tumor cell line, OC-3-VGH, were generated using modified hybridoma technology.
Conclusions: Immunization with oxidized ovarian tumor cell lines may represent an improved therapeutic strategy to stimulate a polyclonal anti-tumor cellular immune response and hence extend remission in ovarian cancer.