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   带电转移 的翻译结果: 查询用时:0.007秒
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带电转移
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  “带电转移”译为未确定词的双语例句
     The simple protein band fishing by cell (SPBFC) technique was employed in finding the protein bands with neurons attached, and then such protein bands, were taken and transferred to nitric cellulose paper for Western hybridization analysis by antibody for NGF, BDNF,NT-3 and GDNF respectively.
     将有神经细胞贴附的各Rf范围凝胶剪下并将胶内各蛋白带电转移至硝酸纤维膜上,用NGF、BDNF、NT-3、GDNF兔抗血清于硝酸纤维膜上分别进行Western杂交。
短句来源
     The Vehicle to Transfer Electric Load for Improving the Reliability of Distribution Network
     提高配网供电可靠性的电力负荷带电转移
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  相似匹配句对
     Transferred Epithet
     转移修饰语
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     The major just involved superficial muscular layer and no metastasis.
     皆无转移
短句来源
     The Vehicle to Transfer Electric Load for Improving the Reliability of Distribution Network
     提高配网供电可靠性的电力负荷带电转移
短句来源
     The Upper Limit on Masses of Charged Neutron Stars
     带电中子星的质量上限
短句来源
     Normal Magnetic Moments of Charged Particles
     带电粒子的正常磁矩
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The phytochrome was purified with a modified Grimm method from etiolated rice (Oryza saliva var. HPGMR nongken 58S) seedlings incubated for 6 days in darkness. The procedures include a hydroxylapatite column chromatography and a DEAE—agrose gel column chromatography, twice of ammonium sulfate precipation, three times of phosphate buffer washing at different volumes, followed by a preci precipition with polyvinylpyrrolidone (PVP). SDS—PAGE electrophoresis proved that the molecular weight of the purified phytochrome...

The phytochrome was purified with a modified Grimm method from etiolated rice (Oryza saliva var. HPGMR nongken 58S) seedlings incubated for 6 days in darkness. The procedures include a hydroxylapatite column chromatography and a DEAE—agrose gel column chromatography, twice of ammonium sulfate precipation, three times of phosphate buffer washing at different volumes, followed by a preci precipition with polyvinylpyrrolidone (PVP). SDS—PAGE electrophoresis proved that the molecular weight of the purified phytochrome was 124 kDa, and the protein can cross—immune with rye phytochrome monclonal antibodies .

以暗室培养6天的湖北光敏感核不育水稻农垦58S黄化芽为材料,采用稍加改进的Grimm等方法,提纯了光敏色素。其分离过程包括羟基磷灰石和DEAE—琼脂糖凝胶柱层析两次梯度洗脱,硫酸铵盐析两次,再经3次不同用量、不同浓度的磷酸钾缓冲液洗涤沉淀,最后用聚乙稀吡咯烷酮沉淀色素,溶于磷酸钾缓冲液中。提取的光敏色素经SDS—PAGE电泳,分子量为124kDa,其电泳谱带电转移后,可以与黑麦光敏色素单克隆抗体发生交叉免疫反应。

Our previous studies have demonstrated that the extract from dorsal horn of embryonic chicken can promote apparently the neurite growth of spinal ganglion during development. In this study, we investigated progressively the neurotrophic factors for primary sensory neurons in spinal dorsal horn. The tissues of spinal dorsal horn from five hundred Hamburger Stage-40 embryonic chickens were taken. After homogenization and centrifugation, the supernatant was subjected to SDS-polyacrylamide gel electrophoresis (SDS-PAGE...

Our previous studies have demonstrated that the extract from dorsal horn of embryonic chicken can promote apparently the neurite growth of spinal ganglion during development. In this study, we investigated progressively the neurotrophic factors for primary sensory neurons in spinal dorsal horn. The tissues of spinal dorsal horn from five hundred Hamburger Stage-40 embryonic chickens were taken. After homogenization and centrifugation, the supernatant was subjected to SDS-polyacrylamide gel electrophoresis (SDS-PAGE ) for analyzing the protein in dorsal horn. The simple protein band fishing by cell (SPBFC) technique was employed in finding the protein bands with neurons attached, and then such protein bands, were taken and transferred to nitric cellulose paper for Western hybridization analysis by antibody for NGF, BDNF,NT-3 and GDNF respectively. The results of SPBFC showed that some neurons were attached to gel within the scope of mobility relative front (Rf) 0. 10-0. 19, 0. 30-0. 35, 0. 50-0. 55, 0. 60-0. 67 and 0. 90-0. 94 respectively, indicating there were some neurotrophic factors for maintaining neuron survival in the above Rf scope. Moreover, the result of Western hybridization showed that the positive hybridization bands in each scope were that of NGF, BDNF, GDNF;NGF,BDNF,NT-3;NGF, BDNF, NT-3; NGF, NT-3 and BDNF, NT-3 respectively;indicating that the neurotrophic effect of spinal dorsal horn tissue on primary sensory neurons involves NGF, BDNF,NT-3 and GDNF. These also suggest that the neuronal survival effect in each Rf scope involves at least two or more neurotrophic factors.

为探讨脊髓背角内影响SG神经元存活和生长的因素,取500只Hamburger40期鸡胚脊髓背角组织匀浆,离心后提取上清液,用SDS-PAGE电泳初步分离脊髓背角内的蛋白质。以简易细胞钓蛋白带(PBFC)技术找寻有神经细胞贴附的蛋白带并求相对迁移率(Rf)。将有神经细胞贴附的各Rf范围凝胶剪下并将胶内各蛋白带电转移至硝酸纤维膜上,用NGF、BDNF、NT-3、GDNF兔抗血清于硝酸纤维膜上分别进行Western杂交。PBFC结果显示:在电泳胶Rf0.10~0.19、0.30~0.35、0.50~0.55、0.60~0.67、0.90~0.94处发现有多少不等的神经元附着。而Western杂交结果表明,在上述各Rf范围均有强弱不等的多条阳性杂交带。分别为NGF、BDNF、GDNF者;NGF、BDNF、NT-3者;NGF、BDNF、NT-3者;NGF、NT-3者、BDNF、NT-3者。提示:40期鸡胚脊髓背角提取液各Rf蛋白带的促神经元存活作用,至少涉及NGF、BDNF、NT-3、GDNF中的两种或三种。

 
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