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  hepatitis b
This study evaluates the survival outcomes of patients who underwent living-donor liver transplantation (LDLT) using right lobe liver grafts for fulminant liver failure due to hepatitis B infection.
      
Expression and Characterization of Hepatitis B Surface Antigen in Transgenic Potato Plants
      
Transgenic potato plants expressing the gene of hepatitis B surface antigen (HBsAg) under the control of the double promoter of 35S RNA of cauliflower mosaic virus (CaMV 35SS) and the promoter of patatin gene of potato tubers have been obtained.
      
The molecular weight of HBsAg peptide was approximately 24 kD, which is in agreement with the size of the major protein of the envelope of hepatitis B virus.
      
Therefore, as well as in recombinant HBsAg-yeast cells, assembling of HBsAg monomers into immunogenic aggregates takes place in HBsAg-transgenic potato, which can be used as a source of recombinant vaccine against hepatitis B virus.
      
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  type b
Synthesis of Extracellular Chitinase by Wild-Type B-10 and Mutant M-1 Strains of Serratia marcescens
      
Molecular weights of extracellular chitinases from wild-type B-10 (62, 54, 43, 38, and 21 kDa) and mutant M-1 strains of Serratia marcescens (62, 52, 43, 38, and 21 kDa) were estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
      
Girls of type A behavior have two extreme types of blood circulation (hypo- and hyperkinetic), while girls of type B behavior are characterized by a eukinetic type of circulation.
      
CNP activates the type B natriuretic peptide receptor (NPR-B), known as the guanylate cyclase B membrane enzyme, which results in the cGMP release.
      
It was found that [Ln(DMF)8][Re6Q7Br7] (Q = S, Se) can be divided into three structural groups: I, II, and VI (type A), VII (type B), and III-V, VIII, IX (type C).
      
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PLO/PRF/5 hepatoma cell line (PLO) adapted to Eagles' medium supplemented with 10% of inactivated calf serum in our laboratory was successfully passaged, and confluent growth was generally observed on the third day after seeding. The production of hepatitis B surface antigen(HBsAg)in tissue culture fluid was serially determined by solid phase radioimmunoassay. Prolonged incubation of culture in growth or maintenance media resulted in a progressive increase in HBsAg, which was maximum around 18 days, and then...

PLO/PRF/5 hepatoma cell line (PLO) adapted to Eagles' medium supplemented with 10% of inactivated calf serum in our laboratory was successfully passaged, and confluent growth was generally observed on the third day after seeding. The production of hepatitis B surface antigen(HBsAg)in tissue culture fluid was serially determined by solid phase radioimmunoassay. Prolonged incubation of culture in growth or maintenance media resulted in a progressive increase in HBsAg, which was maximum around 18 days, and then declined gradually. Compared with highly purified HBsAg prepared from sera of carriers, maximum production of HBsAg from PLO cultures collected between 14-18 days was about 200ng/ml/105 cells. The increase of HBsAg production was paralleled by cytological changes, in which there were degenerations in lysosomes and mitochondria, fusion of cells, and increase in the number of detached and non-viable rounded cells. These were in agreement with the suggestion that HBsAg production is cell cycle related to and representing a terminal event. Depletion of calf serum from media within four days of culture had no significant effect on the production of HBsAg, but after four days cells were found to be detached from flaska, followed by decreased production of HBsAg. When polysaooharides of Eadix Astragali Seu Hedysari (BAH), a Chinese herb which has been shown to prolong the life-span of human embryo lung diploid cell strain was added to culture media, the life-span of PLC, however, was not prolonged, nor was the production of HBsAg enhanced significantly.

PLC/PRF/5肝癌细胞系(PLC)经适应于本实验室含10%小牛血清的Eagles’营养液(csEagles’液),传代3天后即可长成单层。应用固相放射免疫测定法(SPRIA)动态检测产生乙型肝炎表面抗原(HBsAg)量,发观随培养天数延长,HBsAg产量增多,到18天左右达高峰。与纯化HBsAg此较,最高产量(14~18天)约为200ng/ml/10~5细胞。HBsAg产量的增多与PLC细胞出现溶酶体及线粒体退变、细胞融合及脱落死亡圆细胞数增多呈平行关系。这些现象与认为产生HBsAg是PLC生长周期末期活动的看法相符。自营养液中去除小牛血清,则细胞仅能短期维持,第4天起开始脱落,但4天内对HBsAg产量无显著影响。加入黄芪多糖培养,未能延长细胞生长寿命或明显提高HBsAg产量。

The entire observation of biological characteristics on the cultured cells of five human hepatoma (hepatocellular carcinoma) cell lines (PLC/PRF/5, MAH, BEL-7402, QGY-7703, SMMC-7721) was completed. The scope of observation included cell morphology, growth, frozen, preservation in liquid nitrogen and recovery, chromosome counts, and detection of special material (e.g. AFP, HBsAg). The results of our observation confirmed that the cells are of epithelial origin malignant in nature and are able to produce and...

The entire observation of biological characteristics on the cultured cells of five human hepatoma (hepatocellular carcinoma) cell lines (PLC/PRF/5, MAH, BEL-7402, QGY-7703, SMMC-7721) was completed. The scope of observation included cell morphology, growth, frozen, preservation in liquid nitrogen and recovery, chromosome counts, and detection of special material (e.g. AFP, HBsAg). The results of our observation confirmed that the cells are of epithelial origin malignant in nature and are able to produce and secrete AFP. Therefore, they are useful materials for further research on human hepatoma. The PLC/PRF/5 cells preserve the capabilities of producing and secreting HBsAg which existed at the time of establishment of the cell line, and remain to be a special experimental model for the research work on the relationship between hepatitis B virus and hepatocellular carcinoma. The success of introducing cultured cells to consequent transplantation in the nude mice and re-establishing the culture from four different kinds of cell lines provides very useful models for cancer research in the future.

对五株人体肝癌细胞(PLC/PRF/5、MAH、BEL-7402、QGY-7703、SMMC-7721)作了多方面的生物学特性观察,包括形态、生长、冻存与复苏后钓变化、染色体计数、特异物质测定等,证明其为上皮来源,并有恶性特征,能产生与分泌甲胎蛋白,为肝癌研究提供了有用的资料。其中PLC/PRF/5细胞尚保留其建株时原有的产生与分泌乙型肝炎表面抗原(HBsAg)的特性,可继续作为研究乙型肝炎病毒与肝细胞癌关系的一个特殊实验模型。另外四株细胞的裸鼠接种致瘤、连续移植及重建培养的成功,为今后肿瘤研究开创了有利的条件。

Using 125I-UdR labelled K 562 cells as target cell for assaying NK activity in peripheral blood from 29 cases of viral hepatitis B, acute hepatitis 17, chronic persistent hepatitis 9, asymptomatic HBsAg carriers 3 and cirrhosis of liver 2, together with 40 healthyadults as controls. The following results were obtained:mean ± SD of NK activity of the control group was 35.24 ±12.24%; found to be markedly elevated in 5 early acute hepatitis cases (P< 0.001), significantly depressed in 7 chronic persistent hepatitis...

Using 125I-UdR labelled K 562 cells as target cell for assaying NK activity in peripheral blood from 29 cases of viral hepatitis B, acute hepatitis 17, chronic persistent hepatitis 9, asymptomatic HBsAg carriers 3 and cirrhosis of liver 2, together with 40 healthyadults as controls. The following results were obtained:mean ± SD of NK activity of the control group was 35.24 ±12.24%; found to be markedly elevated in 5 early acute hepatitis cases (P< 0.001), significantly depressed in 7 chronic persistent hepatitis and 2 cirrhosis of liver patients (P<0.05), while that of 3 carriers and of 9 convalescents were within normal range. The above results consistent well with the reports of some authors, but not with some of the others. According to our observation the early rise of NK activity in acute infection and the declination in chronic stage was in accordance with the blood interferon level as was observed in some other viral diseases, (e. g. CMV, LCM et al), and also it was in parallel with SGPT level in early stage of acute hepatitis B.

本文用~(125)IUdR 标记的K 562细胞作为靶细胞,检测 29例病毒性乙型肝炎患者(急性期17例、慢迁肝7例、无症状 HBsAg 携带者3例和肝硬化2例)外周血中自然杀伤(NK)细胞的活性。结果发现40例正常对照组的NK活性平均值±SD 为35.24±12.24%;5例急性早期患者显著升高(P<0.001);7例慢迁肝患者明显降低(P<0.05);9例恢复期和 3例无症状 HBsAg携带者均在正常范围内。以上结果与中岛悦朗和Serdengerti的报道相符,但与另些作者的资料有差异。我们认为NK活性在病程初期升高和在慢性期降低与干扰素的生成和乙肝病人早期肝损害有关,与若干种其他病毒性疾病中所见的规律相仿。

 
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