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This study evaluates the survival outcomes of patients who underwent living-donor liver transplantation (LDLT) using right lobe liver grafts for fulminant liver failure due to hepatitis B infection.
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Expression and Characterization of Hepatitis B Surface Antigen in Transgenic Potato Plants
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Transgenic potato plants expressing the gene of hepatitis B surface antigen (HBsAg) under the control of the double promoter of 35S RNA of cauliflower mosaic virus (CaMV 35SS) and the promoter of patatin gene of potato tubers have been obtained.
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The molecular weight of HBsAg peptide was approximately 24 kD, which is in agreement with the size of the major protein of the envelope of hepatitis B virus.
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Therefore, as well as in recombinant HBsAg-yeast cells, assembling of HBsAg monomers into immunogenic aggregates takes place in HBsAg-transgenic potato, which can be used as a source of recombinant vaccine against hepatitis B virus.
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Synthesis of Extracellular Chitinase by Wild-Type B-10 and Mutant M-1 Strains of Serratia marcescens
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Molecular weights of extracellular chitinases from wild-type B-10 (62, 54, 43, 38, and 21 kDa) and mutant M-1 strains of Serratia marcescens (62, 52, 43, 38, and 21 kDa) were estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
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Girls of type A behavior have two extreme types of blood circulation (hypo- and hyperkinetic), while girls of type B behavior are characterized by a eukinetic type of circulation.
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CNP activates the type B natriuretic peptide receptor (NPR-B), known as the guanylate cyclase B membrane enzyme, which results in the cGMP release.
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It was found that [Ln(DMF)8][Re6Q7Br7] (Q = S, Se) can be divided into three structural groups: I, II, and VI (type A), VII (type B), and III-V, VIII, IX (type C).
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| The results of anti-HBC titers in 2041 persons from different population and their clinical application have been reported. | 本文报告了2401例不同人群中抗-HBc滴度测定结果与临床意义。用IAHA(1∶50,1∶100,1∶900,1∶8100)法测定了235例HBsAg阳性和170例健康人抗-HBc。并且,对1475例HBsAg阴性者,408例工作人员及113例HBsAg、抗-HBs均阴性者进行了抗-HBc测定,发现17/17例急乙肝患者(100%),67/72例慢乙肝患者(94.4%),35/42例乙慢迁肝患者(83.3%),10/15例携带者(66.6%)IAHA滴度均≥1:900,P<0.01。同时研究了IAHA滴度与SGPT的关系,17例SGPT≥400者(100%),70/74例SGPT 100-400者(94.6%),103/136例SGPT正常者(75.6%)IAHA滴度亦均≥1∶900。P<0.01。认为如果IAHA滴度≥1:900指示HBV感染的存在或HBV的复制。所以,抗-HBc的检测可以作为今后对乙型肝炎诊断及预后观察的一项指标。 | The peroxidase labeled antibody was prepared by IgG fraction and horseradish peroxidase with a one-step and two-step ghtaraldehyde methods. The enzyme labeled antibodies were used for detection.of the intraeellular viral and rickettsial antigens. light microscopic examination of the neurons in paraffin section of the brain stem and hippoeampus of the infected rnouse showed that the imm~noreaetire Japanese B encephalitis viral antigen distributed in the perikaryon, axon and dentrit-like processes. In the hemolymph... The peroxidase labeled antibody was prepared by IgG fraction and horseradish peroxidase with a one-step and two-step ghtaraldehyde methods. The enzyme labeled antibodies were used for detection.of the intraeellular viral and rickettsial antigens. light microscopic examination of the neurons in paraffin section of the brain stem and hippoeampus of the infected rnouse showed that the imm~noreaetire Japanese B encephalitis viral antigen distributed in the perikaryon, axon and dentrit-like processes. In the hemolymph smear of the natural infected tick the spotted fever rickettsia revealed distinctimmunoperoxidase staining. | 用不同方法提取的抗体lgG与辣根过氧化物酶(HRP)按戊二醛一步法和二步法制备过氧化物酶标记抗体,并用于检出细胞内病毒和立克次体抗原。日本乙型脑炎病毒感染小白鼠脑石蜡切片的酶标抗体染色光镜检查结果表明:病毒抗原分布在脑干和海马回神经元的胞体、轴突和树突样胞浆突起内。自然感染蜱血淋巴涂片中的斑点热立克次体经酶标抗体染色显示清晰的阳性反应。 | 1,146 swab rinse samples of 6 medical institutions in Shanghai were collected from their laboratories and stomatological clinics. The occult blood was traced by benzidine method and hepatitis B surface antigen (HBsAg) was detected by enzyme linked immunosorbent assay (ELISA). Prevalence of serum HBsAg among out-patients was 9.8-14.3％ while in the blood smears was about 5.5％. HBsAg was also detected from the surfaces of tables, basins, needles, hands and some medical apparatus. These findings showed that a variety... 1,146 swab rinse samples of 6 medical institutions in Shanghai were collected from their laboratories and stomatological clinics. The occult blood was traced by benzidine method and hepatitis B surface antigen (HBsAg) was detected by enzyme linked immunosorbent assay (ELISA). Prevalence of serum HBsAg among out-patients was 9.8-14.3％ while in the blood smears was about 5.5％. HBsAg was also detected from the surfaces of tables, basins, needles, hands and some medical apparatus. These findings showed that a variety of objects in hospitals had been contaminated by blood. The presence of HBsAg on various surfaces was directly correlated with the contamination of occult blood. The rate of detection of HBsAg was 3-9％, higher than those without blood contamination (0.9-1.6％). In addition, laboratory experiments verified that HBsAg was relatively stable even after 6 weeks under various environmental conditions. The speed of decline in titers of HBsAg under 25℃ was faster than under 4℃. This contamination might serve as an important role of transmission, therefore, strict disinfection should be practised as a routine in the hospitals so as to further control the spread of viral hepatitis B. | 本文介绍了上海市6个医疗机构中的化验室、口腔科进行HBsAg污染情况调查和稳定性研究结果。在1,146份采样中,门诊病人血检标本的HBsAg阳性率为9.8～14.3％,血玻片的阳性率5.5％,尤其在工作台、水斗表面、口腔科器械、工作人员的手以及“消毒”器械均查到HBsAg。以上情况已成为乙型肝炎传播的一条重要途径,因此,医疗机构中严格执行消毒制度,对控制乙型肝炎的发病有一定的流行病学意义。 | | | | << 更多相关文摘 |
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乙型
hepatitis b
2008 CNKI－中国知网