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固定化氨基酰化酶
相关语句
  immobilized aminoacylase
     Under the optimum conditions, the immobilized aminoacylase on DEAE-E/H was prepared and the specific activity was assayed. It illustrated that the specific activity was up to 1200U/g~1500U/g, and the activity yield was more than 60%.
     在此条件下制备的固定化氨基酰化酶比酶活可达1200U/g~1500U/g,酶活保留率超过60%。
短句来源
     The properties of the immobilized aminoacylase on DEAE-E/H (IME) were investigated. The results showed that the IME had high thermostability.
     结果表明,DEAE-E/H固定化氨基酰化酶具有很好的热稳定性。
短句来源
  “固定化氨基酰化酶”译为未确定词的双语例句
     Immobilizing aminoacylase on macroporous DEAE-D/H resin
     DEAE-D/H树脂固定化氨基酰化酶
短句来源
     Immobilization of Aminoacylase on DEAE-E/H
     DEAE-E/H固定化氨基酰化酶
短句来源
     In order to search better resolution condition, we drew the conclusion as followed: The effect of twice chitin film on Immobilized Enzyme with calcium alginate is transform 20 batchs.
     研究了氨基酰化酶的多种包埋方法,尝试了合成树脂共价交联法固定化米曲氨基酰化酶。 用不覆膜,单次覆膜和两次覆膜的固定化酶进行批次拆分实验比较,两次覆膜改性海藻酸钙凝胶包埋固定化氨基酰化酶效果最好,能反应20个批次。
短句来源
     Besides, the optimum temperature, pH and other factors on the reaction of immobilized enzyme are investigated and decided. It’s sure that the inhibition of substrate is not obvious.
     考察了固定化酶的最适反应温度、pH值、热稳定性等反应条件,确定固定化氨基酰化酶对乙酰-DL-蛋氨酸的拆分没有明显的底物抑制现象。
短句来源
     Effects of degree of cross-linking, amount of poregenic agent and different functionallizing agents on immobilization of aminoa-cylase from Aspergillus oryzae on these polymer carriers were studied.
     研究了交联度、致孔剂用量和不同的功能基化试剂对这些载体固定化氨基酰化酶效果的影响。
短句来源
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  相似匹配句对
     Immobilization of Aminoacylase on DEAE-E/H
     DEAE-E/H固定化氨基酰化
短句来源
     Immobilizing aminoacylase on macroporous DEAE-D/H resin
     DEAE-D/H树脂固定化氨基酰化
短句来源
     When immobilized R.
     固定化后的R.
短句来源
     Liquid Fermentation and Immobilization for Aspergillus Oryzae Aminoacylase
     米曲氨基酰化的液态发酵及其固定化
短句来源
     The Immobilization of Pectinase Encapsulated by Sodium Alginate
     果胶固定化
短句来源
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  immobilized aminoacylase
Isolation and Characterization of Immobilized Aminoacylase from Streptoverticillium olivoreticuli
      
The immobilized aminoacylase column decayed over a very long period of operation, but could be completely reactivated by regeneration.
      
The beads prepared at pH 7.0 exhibited high operational and storage stability with the elimination of enzyme leakage and the immobilized aminoacylase possessed high biological activity.
      
As the immobilized aminoacylase was very stable, the cost of enzyme was markedly reduced compared with that of the soluble enzyme.
      
Continuous optical resolution of acyl-DL-amino acid is eff i- ciently carried out using an immobilized aminoacylase column.
      
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A series of methyl acrylate-divinyl benzene copolymers of different structure were synthesized and functionallized by polyethylene polyamines. Effects of degree of cross-linking, amount of poregenic agent and different functionallizing agents on immobilization of aminoa-cylase from Aspergillus oryzae on these polymer carriers were studied. The best one was chosen from these carriers, the properties of aminoacylase immobilized on it were compared with those of soluble aminoacylase . A column of immobilized aminoacylase...

A series of methyl acrylate-divinyl benzene copolymers of different structure were synthesized and functionallized by polyethylene polyamines. Effects of degree of cross-linking, amount of poregenic agent and different functionallizing agents on immobilization of aminoa-cylase from Aspergillus oryzae on these polymer carriers were studied. The best one was chosen from these carriers, the properties of aminoacylase immobilized on it were compared with those of soluble aminoacylase . A column of immobilized aminoacylase was prepared, N -acyl-DL-Methionine and N-acyl-DL-Phenylanine were resolved continuously for a month with good performance.

合成了一系列不同结构的丙烯酸甲酯—二乙烯基苯交联共聚物,并用多乙烯多胺胺解制得了功能基化交联共聚物。研究了交联度、致孔剂用量和不同的功能基化试剂对这些载体固定化氨基酰化酶效果的影响。比较了固定化氨基酰化酶与溶液酶的酶学性质。用这种载体制成固定化酶柱,对N—乙酰—DL—蛋氨酸和N—乙酰—DL—苯丙氨酸进行连续拆分,得到了很好的效果。

Using crossed experiment method, the influence of casting solutions proportion on the cellulose acetate membrane immobilized acylase was studied, andthe properties of the membrane: bubbling point pressure, aperture, porosity andrate of dankness were characterized. The results showed that when the casting solution proportion is optimum, the yield of enzymatic activity can reach 98.2% and enzyme membrane has proper rate of dankness. The residual activity can get 79.7% after it is used repeatedly for 10 times....

Using crossed experiment method, the influence of casting solutions proportion on the cellulose acetate membrane immobilized acylase was studied, andthe properties of the membrane: bubbling point pressure, aperture, porosity andrate of dankness were characterized. The results showed that when the casting solution proportion is optimum, the yield of enzymatic activity can reach 98.2% and enzyme membrane has proper rate of dankness. The residual activity can get 79.7% after it is used repeatedly for 10 times. and after being stored at 4℃ for 60 days almost no out of activity is observed.

采用正交实验法 ,考察了铸膜液配比对醋酸纤维素固定化氨基酰化酶微孔滤膜的影响 ,对酶膜的泡点压力、孔径、孔隙率及透水速率等性能进行了表征 .结果表明 ,铸膜液组合最佳时 ,酶相对活力产率高达 98 2 % ,酶膜的透水率适当 ,重复使用 10次后仍保留原活力的 79 7% ,而其存放稳定性也大大提高

Diethylaminoethyl hydroethylate (DEAE-E/H), a kind of macroporous weakly basic anion exchanger, was used as carrier to immobilize aminoacylase. Several factors, such as the type of DEAE-E/H, pH, temperature, concentration of free enzyme solution, and so on, were investigated systematically. The results showed that the IME activity was influenced markedly by pH, while affected rarely by temperature in certain range. Finally, the appropriate immobilization conditions were obtained, which were: DEAE-E/H was transformed...

Diethylaminoethyl hydroethylate (DEAE-E/H), a kind of macroporous weakly basic anion exchanger, was used as carrier to immobilize aminoacylase. Several factors, such as the type of DEAE-E/H, pH, temperature, concentration of free enzyme solution, and so on, were investigated systematically. The results showed that the IME activity was influenced markedly by pH, while affected rarely by temperature in certain range. Finally, the appropriate immobilization conditions were obtained, which were: DEAE-E/H was transformed into the type of Ac-, pH was about 6.5, temperature was normal temperature, and the concentration of free enzyme solution was 120U/ml. Under the optimum conditions, the immobilized aminoacylase on DEAE-E/H was prepared and the specific activity was assayed. It illustrated that the specific activity was up to 1200U/g~1500U/g, and the activity yield was more than 60%. DEAE-E/H, as the carrier of immobilization, had such advantages as low cost, good physical property, and simple method of immobilization, therefore it had good industrial potentiality.

以弱碱性大孔阴离子树脂DEAE-E/H为载体固定化氨基酰化酶。通过对影响固定化结果的几个因素,如树脂的离子类型、pH值、温度,以及自由酶液浓度等进行系统研究,得到了适宜的固定化条件:将DEAE-E/H转化为Ac-型;自由酶液浓度120U/ml,pH值6.5;固定化温度为常温。在此条件下制备的固定化氨基酰化酶比酶活可达1200U/g~1500U/g,酶活保留率超过60%。DEAE-E/H作为固定化载体,具有价格低廉,物理性能好,固定化方法简便等特点,具有很好的工业应用前景。

 
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