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小孢子再生植株
相关语句
  plants regenerated from microspores
     Genetic Analysis of Plants Regenerated from Microspores in Chinese Cabbage
     大白菜小孢子再生植株的遗传分析
短句来源
  “小孢子再生植株”译为未确定词的双语例句
     The rate of lived plant regeneration was 74.6%.
     小孢子再生植株成活率可达74.6%。
短句来源
     50%-85% (with the average of 60%) of haploid percentage and 20%-50% (with the mean of 35%) of spontaneous doubling efficiency were obtained from microspore derived plants.
     小孢子再生植株中 ,单倍体和自发二倍体比率分别为 5 0 %~85 % (平均为 6 0 % )和 2 0 %~ 5 0 % (平均为 35 % )。
短句来源
     The ratio of doubled haploid plants derived from treated microspores of 21 genotypes ranged from 23. 94% to 99. 42%.
     21种基因型小孢子再生植株中双单倍体比率为23.94%~99.42%。
短句来源
     One plant(2n=42) was identified among the microspore—derived plants from one Brassica napus cv.Oro×Orychophragmus violaceus hybrid(2n=19~37),in which no O. violaceus chromosomes were detected,so the plant has only B.napus chromosomes.
     从甘蓝型油菜品种“Oro”×诸葛菜杂交后的一个混倍体杂种(2n=19~37)的小孢子再生植株中鉴定出1株2n=42的植株,此混倍体不含有诸葛菜染色体,故2n=42植株的细胞内没有诸葛菜染色体。
短句来源
     The studies included affecting factors to microspore embryogenesis and microspore plants regeneration, the methods of increasing microspore plants regeneration rate , the ploidy identification of microspore plants and the selection of parent homozygous lines.
     品种为试材,采用游离小孢子培养方法,对影响小孢子胚诱导的因素,促进小孢子植株再生的方法,小孢子再生植株的培养,小孢子再生植株倍性的鉴定,以及小孢子再生株纯系的选育进行了研究,获得了下列结果:
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  相似匹配句对
     Isolated-microspore Culture and Plantlet Regeneration in Cabbage(Brassica oleracea L.var.capitata L.)
     结球甘蓝游离小孢子培养及植株再生
短句来源
     Isolated Microspore Culture and Plantlet Formation in Chinese Cabbage(Brassica campestris ssp.pekinensis)
     大白菜小孢子胚诱导和植株再生
短句来源
     Embryogenesis and Plant Regeneration from Isolated Microspore Culture of Kale (Brassica oleracea L. var. acephala DC.)
     羽衣甘蓝的小孢子胚诱导和植株再生
短句来源
     Genetic Analysis of Plants Regenerated from Microspores in Chinese Cabbage
     大白菜小孢子再生植株的遗传分析
短句来源
     Isolated Microspore Culture and Regenerated Plant of Chinese Cabbage
     小白菜游离小孢子培养及其再生植株
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  plants regenerated from microspores
Southern analysis of DNA from four albino barley plants regenerated from microspores by direct embryogenesis revealed the presence of plastid genomes which had undergone deletion or alteration of specific restriction fragments (ΔptDNAs).
      


A system of cultivation of micros-pores mechanically isolated from cereal spikes/florets (Segments of barley spikes, florets of rice and wheat) has been successfully established. A large number of green regenerats have been achieved repeatedly in two barley varieties, green plant regeneration was recovered in a wide compatible rice variety 02428. In three wheat varieties, microspore derived mul-ticellular structures (MCS) and early

以大田及温室生长的植株为材料,成功地建立了直接从禾谷类花器官(大麦穗切段、水稻颖花、小麦小穗)机械游离小孢子的程序及培养系统。从供试的二个大麦材料上重复获得大量游离小孢子再生植株,从一个水稻广亲和品种上得到游离小孢子再生植株,以及从三个小麦品种(系)上获得小孢子形成的多细胞结构(MCS)和早期胚状体(ELS)。相对较长时间的低温预处理有利于提高ELS(大麦)及MCS(小麦)的得率,改善培养物的通气状况,以及提早再分化有利绿色植株再生。

Since 1996, the chromosome doubling of Brassica napus L. haplioid has been studied by treating its in vitro microspores with colchicine. Isolated microspores were treated in NLN media with 10~800 mg/L colchicine for 16~90 h followed by a reculture in the same kind of medium but without colchicine to induce embryos formation. The results show that the ratio range of diploid plants from the 10 crosses is 37.10%~90.12% with the mean of 65.44% by treating their microspores for 48 h with 10 mg/L colchicine....

Since 1996, the chromosome doubling of Brassica napus L. haplioid has been studied by treating its in vitro microspores with colchicine. Isolated microspores were treated in NLN media with 10~800 mg/L colchicine for 16~90 h followed by a reculture in the same kind of medium but without colchicine to induce embryos formation. The results show that the ratio range of diploid plants from the 10 crosses is 37.10%~90.12% with the mean of 65.44% by treating their microspores for 48 h with 10 mg/L colchicine. After the treatments of 50mg/L for 48h, the doubling ratio of 8 crosses is 48.72%~ 97.81 % with the mean of 88.86%. However, the doubling rate reaches 90%~100% in majority of experiments using 100~800 mg/L for 16~48 h. But the embryos yield is very low due to poison to the microspores growth when using colchicine with the concentration of more than 200 mg/L. The results also indicate that all flowers born in diploid plants induced by colchicine treatment of microspores are fertile and may produce a great number of seeds in each plant. Whereas fewer seeds can be harvested from chimeric plants born both fertile and sterile flowers by the method of treatment of microspore derived plants.

用秋水仙碱对 5 2份甘蓝型油菜品系 (种 )和它们的杂种离体小孢子的单倍体二倍化技术进行了研究。从花药中分离出的单核晚期小孢子接种在含 10~ 80 0mg/L秋水仙碱的NLN液体培养基中处理 16~ 90h后转入无秋水仙碱的相同培养基诱导胚状体。结果表明 ,用 10mg/L秋水仙碱处理小孢子 4 8h ,10份材料的双单倍体植株变幅为 37.10 %~ 90 .12 % ,平均为 6 5 .4 4 %。 5 0mg/L处理 4 8h的 8份材料是 4 8.72 %~ 97.81% ,平均88.86 %。 10 0~ 80 0mg/L处理 16~ 4 8h的试验 ,多数加倍率在 90 %~ 10 0 %之间。但该处理药用量大 ,费用高 ,而且对小孢子毒性大 ,胚状体再生率低。试验还表明 ,秋水仙碱处理小孢子诱导的二倍体植株所产生的花一般均能结籽 ,带有不孕花的嵌合植株极少。用秋水碱处理小孢子再生的植株的根或芽所产生的二倍体植株多是可孕和不孕花共生的嵌合植株 ,自交后产生的单株种子很少 ,难以达到遗传和育种所需的群体量。

Flow cytometric (FCM) analysis of ploidy level of microspore derived plants was made in four winter oilseed rape F 1 hybrids. Ploidy could be well determined from fresh, newly born leaf blades at the seedling stage. An unequivocal identification of haploids, diploids, triploids, and tetraploids as well as chimerics such as haploid plus diploid, diploid plus tetraploid, and triploid plus hexaploid, was possible from their discrete peaks of histograms. 50%-85% (with the average of 60%) of haploid percentage...

Flow cytometric (FCM) analysis of ploidy level of microspore derived plants was made in four winter oilseed rape F 1 hybrids. Ploidy could be well determined from fresh, newly born leaf blades at the seedling stage. An unequivocal identification of haploids, diploids, triploids, and tetraploids as well as chimerics such as haploid plus diploid, diploid plus tetraploid, and triploid plus hexaploid, was possible from their discrete peaks of histograms. 50%-85% (with the average of 60%) of haploid percentage and 20%-50% (with the mean of 35%) of spontaneous doubling efficiency were obtained from microspore derived plants. These results were well confirmed by close inspection of the morphological characteristics of rape flowers at flowering stage. The advantage and development of FCM analysis were also discussed in the present paper.

采用流式细胞光度法 (FCM )对甘蓝型冬油菜 4个F1代杂种的小孢子再生植株 ,在幼苗期 (取再生株新长出的叶片嫩组织 )进行了染色体倍数检测。其矩形图上清晰地检测出单倍体、二倍体、三倍体和四倍体以及单倍体加二倍体二倍体加四倍体和三倍体加六倍体等嵌合体。小孢子再生植株中 ,单倍体和自发二倍体比率分别为 5 0 %~85 % (平均为 6 0 % )和 2 0 %~ 5 0 % (平均为 35 % )。FCM倍数检测结果与在油菜花蕾期进行的花器形态鉴别相一致。本文还讨论了采用FCM倍数检测的优势及其检测进展。

 
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