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gag基因     
相关语句
  gag gene
     Expression of HIV2 Gag Gene in Recombinant Vaccinia Virus
     HIV-2gag基因在重组痘苗病毒中的表达
短句来源
     PCR Detection of Lentivirus cDNA GAG Gene in the White cells of sheep
     绵羊白细胞内的慢病毒cDNA GAG基因PCR检测
短句来源
     To construct the recombinant fowlpox virus expression plasmid pUTA-GAG-IL6, IL-6 and HIV-1 gag gene were inserted into the downstream of P7.5 tandem promoter and combined promoter ATI-P7.5 of fowlpox virus expression vector pUTAL respectively.
     分别将IL-6和HIV-1 gag基因插入到鸡痘病毒表达载体质粒pUTAL串联启动子和复合启动子(ATI-P7.5)下游,构建了重组鸡痘病毒表达质粒pUTA-GAG-IL6。
短句来源
     High Level Expression of Human immunodeficiency Virus Type 1 (HIV 1) Gag Gene in Vaccinia Viruses
     HIV-1gag基因重组痘苗病毒的构建和高效表达
短句来源
     Construction of the eukaryotic expression plasmid contaning the gag gene of HIV-I subtype B and its expression in HepG2 cells
     HIV-1B亚型核心蛋白gag基因真核表达载体的构建及其在HepG2细胞中的表达
短句来源
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  partial gag gene
     Cloning and sequencing analysis 5 partial gag gene of jaagsiekte sheep retrovirus strain Inner Mongolia
     绵羊肺腺瘤病毒中国NM株gag基因3'端951 bp段的分子克隆与序列分析
短句来源
     In order to amplify partial gag gene of Jaagsiekte sheep retrovirus Inner Mongolia strain, a pair of primers was designed according to the GenBank sequence . The fragment of gag gene was obtained by polymerase chain reaction (PCR), and the gene was cloned into PMD-18 T vector and identified by EcoR I and Sal I digestion.
     参照GenBank中已发表的绵羊肺腺瘤病毒的基因序列,设计合成一对引物,对绵羊肺腺瘤病毒(Jaagsiekte sheep retrovirus,JSRV)内蒙株的gag基因中主要编码CA蛋白的基因段进行PCR扩增,产物经琼脂糖凝胶电泳分析,呈现一条约897bp的特异条带,将其回收后克隆入pMD-18 T载体中,并进行序列测定与分析。
短句来源
     In order to amplify partial gag gene of Jaagsiekte sheep retrovirus Inner Mongolia Stsain,a pair of primers swere designed according to the Genbank sequence. The fragment of gag gene was obtained by polymerase chain reaction(PCR),then the gene was cloned into PMD-18 T vector and identified by PstI and SalI digestion.
     究参照Genebank中已发表的绵羊肺腺瘤病毒的全基因序列,设计合成一对引物,对JSRV中国NM株的gag基因3'端中主要编码核衣壳(NC)蛋白的基因段进行PCR扩增,产物经琼脂糖凝胶电泳分析,呈现一条约951bp的特异条带,将其回收后克隆入pMD_18T载体中,并进行序列测定。
短句来源
  gene gag
     Preliminary Research of Tomato Transformation with Gene gag and gp120 Mediated by Agrobacterium tumefaciens
     农杆菌介导gag基因和gp120基因转化番茄的初步研究
短句来源
     The protective antigen genes cloning and its sequences analysis The main protective antigen gene gag was cloned and its nucleotide sequences and antigenicity were analyzed for the study on MVV nucleic acid vaccine.
     MVV主要保护性抗原基因的克隆和序列分析目的:针对MVV的主要保护性抗原gag基因(P51)进行克隆、序列分析及抗原性分析,以明确其抗原性,为MVV核酸疫苗的构建奠定分子基础。
短句来源
     The leaves of four tomato cultivars as explants were transformed by Agrobacterium tumefaciens (LBA4404 and EHA105) harboring plasmids carrying gene gag, gp120 and gag-gp120 of HIV-1. 8 transformants was obtained in the experiment, 4 of these transformants with gene gag-gp120, 2 with gene gag, 2 with gene gp120, Transformation frequency was 0.9%.
     通过农杆菌转化法将HIV-1的gag基因、即120基因和gag-gp120嵌合基因导入番茄,获得抗性转化再生植株13株,转化频率为1.4%。
短句来源
     Tomato culture system was established. Tomato was transformed via Agrobacterium tumefaciens (EHA105 and LBA4404) harboring plasmids carrying gag, gp120 and gag-gp 120 genes of HIV-1. Transformants were obtained in the experiment. It was demonstrated that gene gag had been inserted into tomato genome after PCR assay.
     通过农杆菌介导,初步将HIV-1的gag基因、gp120基因和gag-gp120嵌合基因导入番茄,得到了抗性转化再生植株,再生植株PCR检测结果呈阳性,从而建立了良好的番茄遗传转化体系。
短句来源
  gag genes
     Subtype and sequence analysis of the Gag genes among HIV-I strains in Zhejiang Province
     浙江省HIV-I流行毒株GAG基因序列测定和亚型分析
短句来源
     Methods HIV-1 env and gag genes were amplified by nested PCR from uncultured peripheral blood mononuclear cells(PBMCs)obtained from 122 HIV-1 carriers confirmed in Shenzhen.
     方法应用巢式聚合酶链反应技术,对122例在深圳市发现的HIV-1感染者外周血单个核细胞中的env基因和gag基因进行扩增,并对各基因区核苷酸序列进行测定和分析。
短句来源
     Abstract: Objective To construct DNA vaccines with HIV - 2 gp105 and gag genes and evaluate the immunological effect of them.
     目的 构建含 HIV-2 gp 105和 gag基因的核酸疫苗,并评价其免疫效果。
短句来源
     Methods RNA or DNA was extracted from 22 HIV-1 positive samples,HIV env and gag genes were amplified by RT and nested-PCR using specific primer pairs and sequenced directly.
     〔方法〕从22份HIV-1抗体阳性样品中提取RNA或DNA,逆转录后或直接巢式PCR扩增HIV env及gag基因,对PCR产物进行核苷酸序列测定和分析,确定基因亚型。
短句来源
     Methods A recombinant DNA vaccine plasmid was constructed by cloning HIV - 2 gplOS and gag genes into vector pcDNA 3.1 ( + ).
     方法 将 HIV-2 gp 105型和gag 基因克隆到核酸疫苗载体 pcDNA3.l(+)中,构建重组核酸疫苗质粒。
短句来源
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      gag gene
    The CTB-Gag gene fusion was detected in transformed potato leaf genomic DNA by polymerase chain reaction-mediated DNA amplification.
          
    Proviral DNA (gag gene sequences) was found in LC-enriched epidermal cellular DNA from day 4 post-infection with isolate HTLVIII-B and from day 7 with isolate RF.
          
    We studied the expression of the human endogenous retrovirus (HERV)-K gag gene in eight gonadoblastomas arising in phenotypically female patients, including two newborn girls.
          
    In addition to p24 the anti-core mAbs recognized the primary translation product of the viral gag gene p55 and an intermediate cleavage product p41.
          
    Primers for the Taq polymerase were selected in the GAG gene of Icelandic maedi-visna virus and POL gene of caprine arthritis-encephalitis (CAE) virus.
          
    更多          
      gene gag
    Exploiting a published allelic difference in γ-gliadin gene GAG56D, we developed and evaluated three PCR-based methods to determine the proportion of wheat in spelt flour and products.
          
    Expression of v-erbB is accomplished by splicing that joins the first six codons of the viral gene gag to the transduced portion of c-erbB.
          
      gag genes
    The first ORF, 2562 by in length, shows homology to retroviral gag genes.
          
    The first ORF shows homology to retroviral gag genes.
          
    These trees were consistent with those described using Avian sarcoma and leucosis virus gag genes and those from amino acid sequences of hemoglobin and lysozyme c.
          
    MRS of Phodopus sungorus (MRS-Ps) contain regions homologous to the LTR, pol, and, probably, env, but not gag genes of MMTV.
          
    Amino acid sequence and copy number of the different putative zinc finger domains encoded by the gag genes are shown.
          
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    A conserved sequence of human immunodeficiency virus type 1 (HTV - 1) gag gsne was amplified using a selective DNA amplification technique, pdymerase chain reaction (PCR). Using pAG423 that contains gag gene of ARV - 2 as template, by PCR technique, the target sequence of HTV 1 gene could be amplified over 10' times and was identified simply by electro phoresis and ethydium bromide staining. It was found for the first time that the sensitivity of PCR was high enough to detect a few copies of HTV - 1 gene directly....

    A conserved sequence of human immunodeficiency virus type 1 (HTV - 1) gag gsne was amplified using a selective DNA amplification technique, pdymerase chain reaction (PCR). Using pAG423 that contains gag gene of ARV - 2 as template, by PCR technique, the target sequence of HTV 1 gene could be amplified over 10' times and was identified simply by electro phoresis and ethydium bromide staining. It was found for the first time that the sensitivity of PCR was high enough to detect a few copies of HTV - 1 gene directly. The product of amplification was identified by dot blot hybridization with a specific probe. Usually the best amplification was obtained by 30 to 35 cycles of PCR. If unsaturated rounds of amplification was used, quantitative analysis of HIV - 1 provirus was proved possible . The results suggest that PCR is a simple method instead of virus isolation for the determination of HTV -1 infection.

    作者设计了一对DNA引物,用于扩增1型人免疫缺陷病毒(HIV—1)gag基因的一段保守序列。应用聚合酶链反应(PCR)技术,采用含有ARV-2gag基因的亚克隆质粒pAG423作模板,可以使靶基因片段数目增加1×10~7倍以上,经电泳染色后,扩增产物清晰可辨。实验结果表明,这种方法的敏感性极高,足以检测到6.5个拷贝的HIV-1基因。用特异性DNA探针打点杂交证实扩增产物为特异性gag基因片段。实验还表明,经过30~35次PCR循环可以得到最佳扩增效果,而且应用PCR和打点杂交方法还可以对HIV-1基因进行定量分析。因而PCR是一种可以取代病毒分离的常规测定HIV-1感染的简便方法。

    We introduced the cDNA clone of ARV-2 from USA in 1988. We subcloned this full-length cDNA into pUC19 by Kpn I site. Digested with Kpn I, the recombinant plasmid pARV-2/7A was cleaved into 4 fragments. The 6.0 kb fragment was recircularized, and the other three fragments were ligated with pUC19 plasmid cut open by Kpn I. By screening and identification, four subclones were obtained. Of which, pJE332 contains the whole env gene, pJG423 contains LTR, gag and part of pol gene, pJP163 and pJP032 contain only part...

    We introduced the cDNA clone of ARV-2 from USA in 1988. We subcloned this full-length cDNA into pUC19 by Kpn I site. Digested with Kpn I, the recombinant plasmid pARV-2/7A was cleaved into 4 fragments. The 6.0 kb fragment was recircularized, and the other three fragments were ligated with pUC19 plasmid cut open by Kpn I. By screening and identification, four subclones were obtained. Of which, pJE332 contains the whole env gene, pJG423 contains LTR, gag and part of pol gene, pJP163 and pJP032 contain only part of pol gene. These subclone plasmids have been being used in many investigations on gene detection and cloning of HIV type I.

    艾滋病相关病毒(ARV-2)是目前应用最广的艾滋病毒分离株之一.其基因组大小为9737碱基.为便于研究工作,作者对其进行了亚克隆.用Kpn I消化pARV-2/7A产生4个DNA片段,其中含有env基因和pUC19序列的大片段自身环化,得到亚克隆质粒pJE332.其余3个片段分别与pUC19连接,得到另3个亚克隆质粒pJG423,pJP 032和pJP163.其中pJG423含有LTR序列、gag基因和部分pol基因,pJp032和pJP163均插入了部分pol基因.这些亚克隆质粒已被用于各项艾滋病研究.

    In the present paper. the primers PG01 and PG02 were synthesized in which the EcoRI and BamHI recognition sites as well as the initiation and termination codons were designed for convenience of subsequent cloning and expression. Micrograms of target gene sequence was produced by 30 rounds of amplification with pJG 423 as a template. The amplified gene fragment was digested with both EeoRI and BamHI and ligated to pUC19 predigested with the same restriction endonucleases. The recombinant plasmid pCY52 was identified...

    In the present paper. the primers PG01 and PG02 were synthesized in which the EcoRI and BamHI recognition sites as well as the initiation and termination codons were designed for convenience of subsequent cloning and expression. Micrograms of target gene sequence was produced by 30 rounds of amplification with pJG 423 as a template. The amplified gene fragment was digested with both EeoRI and BamHI and ligated to pUC19 predigested with the same restriction endonucleases. The recombinant plasmid pCY52 was identified and the cloned P20 gene was inserted into phage M13mpl8 for sequence analysis. It was revealed that the sequence of the cloned P20 gene (471bp) was identical to, its template. And the site mutations at both the 5′ and 3′ ends were also successful.

    作者设计并合成了一对突变引物PGO1和PGO2,分别在两引物中设计了两个突变点,使突变后基因含有EcoRI、BamHI和ATG及TAA序列,以便于HlV-1gag基因序列的定向克隆和表达。用PCO1和PGO2作引物,采用PCR方法从HIV-1基因组DNA中扩增出一个长504bp的DNA片段,用EcoRl和BamHI双酶切位点将此片段定向克隆入pUC19质粒。将克隆基因插入M13mp18进行DNA序列分析,结果表明,该基因序列及读框完全正确,且在其5′末端突变出EcoRI位点和ATG起始码,3′末端突变出TAA终业码和BamHI位点,从而为该基因的表达研究奠定了基础。

     
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