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家蚕细胞表达
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  “家蚕细胞表达”译为未确定词的双语例句
     Hepatitis B Virus e Gene Expression in Bombyxmori Cells and Application of HBeAg
     用家蚕细胞表达HBeAg及其应用
短句来源
     The cell growth index was 7.9. At 48-60 hours after cell passage, the Bm N cells was inoculated with rBmHBe (multiplicity of infection Mol=0.4 PFU/cell). Five days later the HBeAg titer in the medium was 1∶96000 and in the control group it was 1∶32000.
     在细胞指数生长期(传代后48~60h)用载有HBeAg基因的重组病毒(rBmHBe)接种(感染量为0.4PFU/细胞),5d后,培养上清中HBeAg滴度为1∶9.6×104,是用方瓶静止培养的家蚕细胞表达量的3倍。
短句来源
     SDS-PAGE and western blotting analysis performed that the molecular weight of recombinant S protein was about 30 kD in silkworm. The pure recombinant S protein expressed by baculovirus could be easily obtained through one-step metal chelate affinity chromatographer.
     SDS-PAGE、W estern b lotting分析表明,表达产物的分子量约30 kD,用金属螯合亲和层析纯化得到家蚕细胞表达的重组S蛋白。
短句来源
     Neomycin resistance gene driven by ie-1 promoter element of Bombyx mori nucleopolyhedrorvirus was cloned into insect transposon vector piggyBac,which contains gfp report gene,to form transgenic vector pigA3-IE-Neo. Then it was used to transfect BmN cell.
     为探讨稳定转化家蚕细胞表达外源基因的新方法,以家蚕核型多角体病毒(BmNPV)极早期蛋白基因(ie-1)启动子元件驱动新霉素抗性基因(neor),并克隆至具有绿色荧光蛋白基因(gfp)标记的昆虫转座子载体piggyBac中,构建转基因载体pigA3-IE-Neo。
短句来源
  相似匹配句对
     Hepatitis B Virus e Gene Expression in Bombyxmori Cells and Application of HBeAg
     用家蚕细胞表达HBeAg及其应用
短句来源
     EXPRESSION OF GREEN FLUORESCENT PROTEIN IN Bombyx mori CELLS
     绿色荧光蛋白在家蚕细胞中的表达
短句来源
     Then the HEVp166 was expressed in BmN cells and silkworm pupae.
     并在家蚕细胞家蚕蛹中表达p166。
短句来源
     cDNA Cloning of Human Lactoferrin and its Expression in BmN Cells
     人乳铁蛋白cDNA的克隆及在家蚕细胞中的表达
短句来源
     Expression of Human Endostatin in Cultured Cells and Larvae of Silkworm
     人内皮抑素在家蚕细胞和幼虫中的表达
短句来源
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? Hepatitis B Virus e Ag was expressed in Bombyxmori cells infected by recombinant virus,and was compared with HBeAg from E,coli.The results show that enzyme labelled HBeAg from BmN was wed to elect HBe antibody,there were all positive in positive samples,but 2 positive in negative samples which detected by eraditional methods,and 2 positive in 42 samples which were negative detected by HBcAb and HBeAb.Meanwhile,the results in Fig1 referred that in the same concentration,antigenicity of HBeAg from BmN was...

? Hepatitis B Virus e Ag was expressed in Bombyxmori cells infected by recombinant virus,and was compared with HBeAg from E,coli.The results show that enzyme labelled HBeAg from BmN was wed to elect HBe antibody,there were all positive in positive samples,but 2 positive in negative samples which detected by eraditional methods,and 2 positive in 42 samples which were negative detected by HBcAb and HBeAb.Meanwhile,the results in Fig1 referred that in the same concentration,antigenicity of HBeAg from BmN was 100times as high as that from E.Coli.It demonstrate the HBeAg from BmN cells get higher titer and specificity than that from E.coli.

对经重组病毒感染的BmN细胞的表达产物HBeAg进行实验研究,并与菌产HBeAg进行了比较。结果表明用家蚕细胞表达的HBeAg分别作为捕获和酶标抗原建立的检测抗HBe方法,不仅传统方法检测的阳性标本全部阳性,而且在传统方法阴性(抗HBc阳性)组中,检出2例阳性,在抗HBe和抗HBc全部阴性的42例标本中,也发现2例阳性。说明本法的敏感性高于传统方法。同时,用此方法制备的HBeAg,在相同的蛋白浓度下,其抗原反应性也比用原核细胞表达的HBeAg高出100倍,且与抗HBc交叉反应低100倍,表明用家蚕细胞表达的HBeAg不但效价高,且特异性、敏感性均优于大肠杆菌表达系统产生的HBeAg。

Distribution of silkworm Bm N cell attachment to Cytodex 3 microcarrier was observed and the influence of cell inoculation concentration and microcarrier concentration on cell growth was studied. The minimal cell inoculation concentration was 1.0×10 5 cells/mL when Cytodex 3 microcarrier concentration was 3g/L. Cultured under the proper conditions (inoculation concentration: 3.6×10 5 cells/mL; microcarrier cytodex 3 concentration: 5g/L) for 5 days, the final cell concentration of BmN was 2.8×10 6 cells/mL....

Distribution of silkworm Bm N cell attachment to Cytodex 3 microcarrier was observed and the influence of cell inoculation concentration and microcarrier concentration on cell growth was studied. The minimal cell inoculation concentration was 1.0×10 5 cells/mL when Cytodex 3 microcarrier concentration was 3g/L. Cultured under the proper conditions (inoculation concentration: 3.6×10 5 cells/mL; microcarrier cytodex 3 concentration: 5g/L) for 5 days, the final cell concentration of BmN was 2.8×10 6 cells/mL. The cell growth index was 7.9. At 48-60 hours after cell passage, the Bm N cells was inoculated with rBmHBe (multiplicity of infection Mol=0.4 PFU/cell). Five days later the HBeAg titer in the medium was 1∶96000 and in the control group it was 1∶32000.

选择微载体Cytodex3对家蚕BmN(从SilkwormBombyxmori获得的细胞系)细胞进行高密度培养,并获得成功。观察了家蚕BmN细胞在微载体Cytodex3上的贴壁分布,研究了不同接种浓度与微载体浓度时细胞的生长情况,并筛选出最佳的技术参数。在1000mL滚瓶中用微载体Gytodex3培养家蚕细胞,在合适的培养条件下(细胞接种浓度3.6×105细胞/mL、微载体浓度5g/L),5d后,细胞的终密度达到2.8×106细胞/mL,细胞增长指数为7.9。在细胞指数生长期(传代后48~60h)用载有HBeAg基因的重组病毒(rBmHBe)接种(感染量为0.4PFU/细胞),5d后,培养上清中HBeAg滴度为1∶9.6×104,是用方瓶静止培养的家蚕细胞表达量的3倍。

A method for isolation and purification of GM CSF from hemolymph fluid of bombyx mori has been developed. After centrifugation and filtration, the hemolymph fluid of bombyx mori was separated by DEAE Sepharose FF chromatography. The interested fraction was determined by Indirect BA ELISA. Then, the pool was purified by Sephadex GM 75 2 gel filtration chromatography. Finally, the desalted pool of interested fraction was purified twice with rotatory isoelectric focusing electrophoresis, yeilding over 97% pure...

A method for isolation and purification of GM CSF from hemolymph fluid of bombyx mori has been developed. After centrifugation and filtration, the hemolymph fluid of bombyx mori was separated by DEAE Sepharose FF chromatography. The interested fraction was determined by Indirect BA ELISA. Then, the pool was purified by Sephadex GM 75 2 gel filtration chromatography. Finally, the desalted pool of interested fraction was purified twice with rotatory isoelectric focusing electrophoresis, yeilding over 97% pure rhGM CSF and overall recovery of 33.59%. This study also provided useful parameters for massproduction and clinical use of rhGM CSF.

为探索适于昆虫表达体系重组蛋白质的分离、纯化方法,对家蚕细胞表达的重组人粒细胞-巨噬细胞集落刺激因子(rhGM-CSF)的纯化工艺进行了研究。利用离子交换色谱、凝胶过滤色谱及旋转等电聚焦电泳等技术,建立了从家蚕血淋巴液中分离、纯化rhGM-CSF的工艺路线。经过三步分离,获得了纯度为97.24%的目标产品,总回收率达33.59%。此法可有效地去除动、植物色素且简单、经济、高效,适于进行规模放大,为rhGM-CSF的大规模制备及临床应用打下了基础。

 
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