Results The TNF positive expression was lower in the auto-orthotopic transplantation group and the liquid nitrogen refrigeration group than that in the fresh transplantation group and the antibiotic-treated group. And compared with that in the other groups, the positive expression of TNF was highest in the fresh transplantation group, with statistical significance (P<0.05).
After vasotransplantation, the findings of myocardium were analogous to those of blocd vessels. TNF expression was higher in the fresh transplantation group than that in the other groups, with a significant difference from that in the auto-orthotopic transplantation group and liquid nitrogen refrigeration group (P<0.05), but not from that in the antibiotic-treated group.
The authors have done 29 cases (61 pieces) of autologous epidermis-grafting for vitiligo on faces and necks with a self-made vacuum epidermis separation equipment in combination with epidermal separation by liquid nitrogen cryotherapy(ESLNC).
Conclusion The curative effect is good that acuity condyloma was treated with liquid nitrogen cryotherapy and transfer gene,and the recrudescence rate is low,the handle is simple. It is a efficiency and safety means.
The samples were homogenated or ground to powder after freezing with liquid nitrogen. 1%triton 100 and certain volume of ethylacetate isopropanol (10∶1) were added, shaken and centrifuged. Then the entire organic layer was transferred to a tube and vacuum dried.
Methods A retrospective analysis was performed in 121 patients who underwent surgical treatment for giant cell tumor close to the knee between 1978 and 1997.There were 71 cases had been managed with an intralesional procedure of the tumor (curettage, freezing with liquid nitrogen and autograft or allograft, Group 1), 50 cases with a semi-arthroplasty using allograft after en bloc resection (Group 2).