Result:In dogs treated with PQDS(in a dosage of 10 and 20 mg·kg -1 iv infusion),the myocardial infarct size, the activity of serum CK ,LDH and the contents of serum FFA and LPO were decreased,whereas the activity of serum SOD and GSH Px increased markedly.
The myocardial infarct size was measured by TTC staining of cardiac muscle cells in another 24 rats (divided into 3 groups: model group, Daidzein 5.0 mg/kg group, Daidzein 2.5 mg/kg group) with myocardial ischemia injury induced by the same methods.
Immunohistochemiscal stainning revealed that the engrafted stem cells expressed positive cTnI and GATA4.Pathological imaging analysis showed that the area of myocardial infarction was reduced[(38.01±3.40)%vs(43.99±3.50)%,t=4.181,P< 0.05].
Methods Sprague-Dawley rats were randomly divided into two groups. One was 30 min reperfusion group, which was subdivided into sham (n=5), model (vehicle, n=6) and pioglitazone 3 mg/kg (n=7) with 30 min ischemia followed by 30 min reperfusion to detect the area of myocardial infarction (MI).
There were significant differences (P <0. 05 or P <0. 01). The peak value of ET had positive correlation with that of MDA and the area of myocardial infarction (P <0. 01). The peak value of MDA had positive correlation with the area of myocardial infarction (P <0. 01).
Results Xingtongling spray could reduce the area of myocardial infarction and decrease the activities of serum CK, LDH and GOT and improve ischemic ECG changes in rats with myocardial ischemia(t D=1.223-13.890,P<0.05,0.01).
①Four weeks after transplantation the size of the myocardial infarction area in the alliance was (27.8±3.0)%, which was lower than that in the cell group (37.0±10.1)% (P=0.035) and the gene group (37.1±5.2)% (P=0.033).
The no-reflow area (NRA) were determined by injection of thioflavin S into the auticle of left atrium at the end of reperfusion,and the Myocardial infarction Area(MIA) were determined by triphenyltetrazolium(TTC) staining .
观察指标:(1) 心肌无复流面积(No-reflow Area NRA)和心肌梗死面积(Myocardial infarction Area MIA):在血运重建后60min时,以Thioflavin S荧光染色法显示NRA;
~(99m)Tc-MIBI SPECT was performed to detect myocardial infarction area and ~(99m)Tc gated cardiac blood pool image was performed to detect left ventricular ejection fraction(LVEF) at 2 weeks after PCI.
Myocardial ischemia reperfusion injury models were established by the ligation of left desending coronary artery for 30min and reperfusion for 90min in rats and the influence on scores of arrhythmia,myocardial infarction size,cardiac function,the activities of superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GSH-Px),Na~(+)-K~(+)-ATPase,Ca~(2+)-ATPase,and the content of malondialdehyde(MDA) were determined.
RESULTS In DK-treatment group,the score of arrhythmia decreased,the myocardial infarction size diminished,the cardiac function was markedly improved,the activities of SOD,CAT,GSH-Px,Na~(+)-K~(+)-ATPase and Ca~(2+)-ATPase significantly increased while MDA content reduced markedly in myocardium compared with the model control group.
In diabetic condition, the necrotic area of myocardial infarction was significantly extended and myocardial oedema failed to develop after administration of norepinephrine 2 or 48 hours after ligation of the left anterior descending coronary artery.
The data obtained on hearts from rats with experimental myocardial infarction show that estrogens and their nitro derivatives restrict the area of myocardial infarction due to antiischemic and/or antinecrotic activities.
Verapamil and amlodipine produced a potent antiischemic effect and reduced the area of myocardial infarction in rats.
Low-energy laser irradiation for 15 min in vitro decreased the area of myocardial infarction, slightly reduced hemoglobin affinity for oxygen, and inhibited lipid peroxidation.
Finally, the sTNFR1 expression-plasmid treatment significantly reduced the area of myocardial infarction at 2 days after ischemia/reperfusion compared to LacZ plasmid.
However, this treatment significantly reduced the myocardial infarction size, and markedly decreased the contents of plasma cTnT, CK and LDH.
This has been demonstrated to be an accurate means of determining myocardial infarction size in an animal model and is currently being used in a number of centers in patients at the time of cardiac catheterization.
Effect of methylprednisolone on predicted myocardial infarction size in man.
Statins Inhibit Myocardial Infarction Size We next explored the effect of statins on myocardial infarction in mice.