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asd基因
相关语句
  asd gene
     Cloning and sequencing of Shigella flexneri 2a asd gene
     痢疾福氏2a asd基因的克隆及其序列分析
短句来源
     On the basis of the flanking sequences of E. coli K 12 strain asd gene,a pair of primers were designed.
     本文根据大肠杆菌 (E .coli)K12asd基因两侧序列设计了一对引物 ,用全菌PCR扩增了福氏 2aT32株的asd基因及其两侧序列。
短句来源
     A host plasmid balancing system was established based on asd gene in a candidate vaccine strain(T32) of Shigella flexirie 2a.
     首先通过体内外重组的方法,构建了福氏2a痢疾菌T32asd基因缺陷的突变体FaD,作为抗原载体菌;
短句来源
     In this study, SL7207(asd-), the asd gene deletion mutant of the S. typhimurium strain (SL7207), was constructed by homologous recombination. The asd gene deletion mutant SL7207(asd-) was characterized by comparing its surviving and replicating both in vivo and in vitro with its parental strain SL7207.
     本研究通过同源重组的方式,构建了鼠伤寒沙门氏菌SL7207 asd基因突变株SL7207(asd-),并通过比较SL7207与SL7207(asd-)在体内、体外的生长、增殖情况,对SL7207(asd-)的部分生物学特性进行研究。
短句来源
     Methods:An asd gene_based host_plasmid balancing system was used to express CS6 and CTB in a genetic avirulent Salmonella typhi strain, in which CS6 and CTB can be expressed stably in the absence of antibiotic selection.
     方法 :以基因工程减毒的人伤寒沙门菌为抗原载体 ,利用基于asd基因功能互补的宿主染色体_质粒平衡致死表达系统 ,实现了在没有抗生素选择压力的条件下 ,CS6和CTB在人伤寒沙门菌中的稳定表达。
短句来源
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  “asd基因”译为未确定词的双语例句
     Construction of Δasd mutant of Shigella flexneri 2a strain T32
     弗氏志贺菌2a T32株asd基因缺失突变体的构建
短句来源
     Construction of E.coli DH10B Δasd Using Red Recombineering
     应用Red重组工程技术建立asd基因缺失的大肠杆菌DH10B菌株
短句来源
     asd-p VAX1-EGFP was transformed into asd S. typhimurium X4550 and in vitro and in vivo tests showed asd-pVAX1-EGFP can be stably maintained within X4550. When X4550(asd-pVAX1 -EGFP) was used to infect COS-7 cells, EGFP positive COS-7 cells can be observed 24 hours post-infection.
     将质粒asd-pVAX1-EGFP转化进asd基因缺失型沙门氏菌X4550,体外和体内稳定性实验表明该质粒可在X4550内稳定存在。 将X4550(asd-pVAX1-EGFP)感染COS-7细胞,36小时后荧光显微镜下可看到发绿色荧光的COS-7细胞。
短句来源
     The genes encoding S. sonnei form I antigen and CT-B antigen of V. cholerae were inserted into asd+ vector pYA248. The resulting recombinant plasmid pMGL105 was transformed into an attenuated 5. typhimurium asd- strain X4072, and constituted a balanced-lethal host-vector system.
     将编码宋内氏痢疾菌(Shigella sonnei)I相O抗原的基因和霍乱弧菌(Vibrio choler-ae)的CT-B基因克隆至带asd基因的质粒PYA248,得重组质粒PMGL105。
短句来源
     In this study,we knockin inv gene from Yersinia seudotuberculosis, hly gene from Listeria monocytogenes and P4 gene from trypanosome cruzi into E. coli DH10B chromosome using homologous recombination technology.
     本研究应用Red重组工程技术将耶尔森氏菌(Yersinia seudotuberculosis)inv基因、李斯特菌(Listeria monocytogenes)hly基因、南美锥虫(trypanosome cruzi)P4基因敲入大肠杆菌染色体中,并将大肠杆菌染色体asd基因进行缺失,构建出四株细胞壁合成营养缺陷型侵袭性E.
短句来源
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  相似匹配句对
     4 new genes were obtained.
     U基因
短句来源
     Objective\ To obtain the asd gene of E.
     目的获得大肠杆菌全长asd基因
短句来源
     coli lac Z gene.
     colilacZ基因
短句来源
     Cloning and sequencing of the asd gene of Salmonella typhimurium
     鼠伤寒沙门氏菌asd基因的克隆及序列分析
短句来源
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  asd gene
glutamicum asd gene complemented Escherichia coli asd mutants.
      
The insertions had no adverse effect on expression of the downstream asd gene but resulted in leucine auxotrophy.
      
One clone, which carried the asd gene and expressed Asd activity, was isolated and chosen for further study.
      
Transformants with this ligation were screened for replacement ofthe central 1.6-kb PstI fragment that contains the asd gene.
      
Therefore, we favor the interpretation that the asd gene is essential in mycobacteria.
      


The genes encoding S. sonnei form I antigen and CT-B antigen of V. cholerae were inserted into asd+ vector pYA248. The resulting recombinant plasmid pMGL105 was transformed into an attenuated 5. typhimurium asd- strain X4072, and constituted a balanced-lethal host-vector system. In the system, the plasmid was stable and had no drug resistance gene. A series of tests showed that the recombinant strain could express both 5. sonnei form I antigen and V. cholerae CT-B antigen stably. Immune protection experiments...

The genes encoding S. sonnei form I antigen and CT-B antigen of V. cholerae were inserted into asd+ vector pYA248. The resulting recombinant plasmid pMGL105 was transformed into an attenuated 5. typhimurium asd- strain X4072, and constituted a balanced-lethal host-vector system. In the system, the plasmid was stable and had no drug resistance gene. A series of tests showed that the recombinant strain could express both 5. sonnei form I antigen and V. cholerae CT-B antigen stably. Immune protection experiments in mice indicated that the recombinant strain could provide good protections against the challenge of virulent S. sonnei and V. cholerae.

将编码宋内氏痢疾菌(Shigella sonnei)I相O抗原的基因和霍乱弧菌(Vibrio choler-ae)的CT-B基因克隆至带asd基因的质粒PYA248,得重组质粒PMGL105。将该重组质粒转入asd基因缺失的减毒伤寒沙门氏菌X4072,构成了一个不带抗药性基因的载体-宿主平衡致死系统。一系列实验表明,该重组菌X4072(PMGL105)能稳定地表达宋内I相O抗原和霍乱弧菌的CT-B抗原。小鼠免疫保护实验表明,该重组菌对有毒的宋内氏I相痢疾杆菌及霍乱弧菌的攻击均具有良好保护作用。

The E. coli cs3 gene coding for CFA/11 antigen was site-specifically integrated into the asd gene locus of S. flexneri 2a vaccine strain T32, resulting in the asd gene inactivated. Meanwhile, the S. sonnei O antigen gene was cloned into a non-resistant expression vector pXL378 to construct the plasmid pXL390. By transforming the asd minus T32 strain with pXL390, the bivalent vaccine candidate strain FS01 against S. flexneri 2a and S. sonnei was finally obtained. Experiments showed that the recombinant plasmid...

The E. coli cs3 gene coding for CFA/11 antigen was site-specifically integrated into the asd gene locus of S. flexneri 2a vaccine strain T32, resulting in the asd gene inactivated. Meanwhile, the S. sonnei O antigen gene was cloned into a non-resistant expression vector pXL378 to construct the plasmid pXL390. By transforming the asd minus T32 strain with pXL390, the bivalent vaccine candidate strain FS01 against S. flexneri 2a and S. sonnei was finally obtained. Experiments showed that the recombinant plasmid pXL390 was very stable in the asd minus T32 strain without the use of any antibiotics; FS01 strain was genetically stable and expressed two kinds of Shigella LPS-O antigens with no enhancement of its toxicity. Animal test demonstrated that FS01 strain, when administered subcutaneously in mice,could confer 100% protection against the intraperi-toneal challenges of virulent S. flexneri 2a and S. sonnei.

通过体内外基因重组,将大肠杆菌粘附因子cs3基因定位整合到痢疾杆菌福氏2a疫苗株T32菌染色体的asd基因内,使asd基因灭活;将来内O抗原基因克隆至无抗药性表达载体pXL378,获得重组质粒pXL390,将其转化asd-的T32受体菌,构建成福氏2a和宋内双价苗苗株FS01。实验表明:重组质粒pXL390在不带任何抗菌素基因的情况下,在asd-的T32受体菌内是稳定的。FS01株遗传稳定,能表达两种痢疾菌的PLS-O抗原,无明显毒性作用。动物试验表明,以FS01株皮下免疫的小鼠对福氏2a和宋内有毒株的腹腔攻击有100%的保护。

The genes encoding S.sonnei form I O antigen and V.cholerae B subunit were cloned into an expression vector containing as.gene of Streptococcus mutans by gene recombination.The final recombination plasmid was transformed into the asd mutant of S.flexneri 2a(strain T32).Analysis of LPS silver staining and western blotting indicated that the genes encoding CT-B and form I O antigen could stably express in the asd mutant T32 strain.Immune protection tests in mice showed that the recombinant strain constructed in...

The genes encoding S.sonnei form I O antigen and V.cholerae B subunit were cloned into an expression vector containing as.gene of Streptococcus mutans by gene recombination.The final recombination plasmid was transformed into the asd mutant of S.flexneri 2a(strain T32).Analysis of LPS silver staining and western blotting indicated that the genes encoding CT-B and form I O antigen could stably express in the asd mutant T32 strain.Immune protection tests in mice showed that the recombinant strain constructed in this study could provide 100% protection against the challenge from S.flexneri 2a and S.sonnei and also showed a good immune protection(70%)against V.cholerae.This recombinant strain has the following adventages:trivalent,stable and no vector drug resistance markers.

本文利用基因重组的方法,将宋内I相O抗原基因以及霍乱毒素B亚单位基因(ctx-B)克隆至带链球菌的asd基因的表达载体,然后转化至asd-痢疾菌苗株福氏2aT32。脂多糖银染以及Westernblotting实验证实以上两基因都能在宿主菌中稳定表达。动物(小白鼠)保护实验表明,该重组菌对福氏2a、宋内氏痢疾菌的保护效率达100%,对霍乱弧菌的保护效率也达70%。该菌具有稳定、无抗生素标记、多价的特点。

 
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