1. Comparison of extraction RNA methods from the cotton fibersThe comparison of three methods of RNA isolation with each other was conducted to isolate high-quality RNA from cotton fiber cells.
The cotton fiber cells contain a lot of endogenous phenolics, polysaccharides and secondary metabolites that may result in difficulty of RNA isolation from fiber cells.
The cell wall components of cotton fiber are synthesized and transported by a functionally-integrated membrane system of endoplasmic reticulum, Golgi complex, and plasmalemma.
By a combination of two rounds of reverse Northern hybridization and Northern blot analyses, a number of such cDNA fragments were proved to represent fiber-specific/preferential genes. Sequencing determination and database searching indicated that most of these genes are novel.
This work was undertaken in order to extend studies concerning nutritional effects and regulation of nucleotide sugar incorporation into β-1,3- and β-1,4-glucan components of cotton fiber cell walls.
We report here the cloning and characterization of a receptor-like kinase gene (designated GhRLK1), expression of which is induced during the period of active secondary wall synthesis in the cotton fiber cells.
As a step towards characterizing protein components of the plant cytoskeleton, we have isolated cytoskeletons from cytoplasts (anucleate protoplasts) prepared from cotton fiber cells grown in ovule culture.
Optical microphotos illustrate the formation process of the fiber cell wall at different stages of growth and noncontractile motion in cotton fiber cells.
The cotton fiber cells contain a lot of endogenous phenolics, polysaccharides and secondary metabolites that may result in difficulty of RNA isolation from fiber cells. In order to isolate high quality RNA from cotton fiber cells the comparison of three methods of RNA isolation with each other was conducted. The comparison analysis show that the method by using a extraction buffer with high ion density and pH value was better. This extraction buffer with high ion density and ph value was characterized by...
LIM domain protein plays an important role in various cellular processes,including construction of cytoskeleton,transcription control and signal transduction.Based on cotton fiber EST database and contig analysis,the coding region of a cotton LIM domain protein gene ( GhLIM1 ) was obtained by RT PCR from 4DPA (day post anthesis) ovule with fiber.The cloned fragment of 848 bp contains an open reading frame of 570 bp,coding for a polypeptide of 189 amino acids.It was demonstrated that the deduced GhLIM1 ...
LIM结构域蛋白是一个重要的发育调控因子 ,参与基因转录、细胞骨架建成和信号传导等许多发育调控过程。胞质骨架是形成和稳定细胞形态以及传递物质、能量和信息的重要成分。为研究棉花纤维细胞发育过程中胞质骨架的形成和作用机理 ,通过棉花纤维EST序列整合 ,从陆地棉徐州 14 2胚珠 (含纤维 )中扩增并克隆出棉花LIM结构域基因的编码区段。该棉花LIM结构域基因 (GhLIM1)长 84 8bp ,包含一个 5 70bp的开放阅读框 ,推导的氨基酸序列 (189个氨基酸 )与拟南芥、烟草和向日葵的LIM结构域蛋白有极高的同源性 ,而且两个LIM结构域完整。每个LIM结构域具有植物LIM结构域共有的双锌指结构C X2 C X17~ 19 H X2 C X2 C X2 C X16~ 2 4 C X2 H。RT PCR和Northern杂交分析表明 ,该基因 (GhLIM1)在陆地棉的根、茎尖、下胚轴、叶片、花蕾、花药、胚珠和不同发育时期的陆地棉纤维 (4DPA、12DPA、18DPA)以及海岛棉纤维 ...
Fluorescence differential display (FDD) technique was used to identify genes that are specifically or preferentially expressed in different developmental stages of cotton fiber cells. One hundred and nine differentially displayed cDNA fragments were isolated using 9, 21 and 27 DPA (days postanthesis) fibers as experimental materials. By a combination of two rounds of reverse Northern hybridization and Northern blot analyses, a number of such cDNA fragments were proved to represent fiber-specific/preferentia...