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      棉花纤维细胞
相关语句
  cotton fiber cells
     Initiation of Cotton Fiber Cells and the Effects of Temperature and Plant Growth Substances on It
     棉花纤维细胞起始及温度、植物生长物质对其影响
短句来源
     1. Comparison of extraction RNA methods from the cotton fibersThe comparison of three methods of RNA isolation with each other was conducted to isolate high-quality RNA from cotton fiber cells.
     一、棉纤维细胞RNA提取方法的比较研究 为高质量提取棉花纤维细胞的RNA,进行了三种提取方法的比较研究。
短句来源
     The cotton fiber cells contain a lot of endogenous phenolics, polysaccharides and secondary metabolites that may result in difficulty of RNA isolation from fiber cells.
     棉花纤维细胞含有高的棉酚、多糖和次生代谢物质 ,对其 RNA的提取造成困难。
短句来源
     In order to isolate high quality RNA from cotton fiber cells the comparison of three methods of RNA isolation with each other was conducted.
     为高质量提取棉花纤维细胞的 RNA,进行了三种提取方法的比较研究。
短句来源
  “棉花纤维细胞”译为未确定词的双语例句
     The analysis show that the method by using an extraction buffer with high ion-density and pH value was better.
     研究表明,用高离子、高pH值提取液提取棉花纤维细胞的RNA比TRIZOL与异硫氢酸胍两种提取液更适合。
短句来源
     The process of fiber development is involved in a series of reactions of polysaccharide biosynthesis.
     棉花纤维细胞发育过程涉及大量的由单糖聚合成多糖的糖苷转移反应。
短句来源
     The cell wall components of cotton fiber are synthesized and transported by a functionally-integrated membrane system of endoplasmic reticulum, Golgi complex, and plasmalemma.
     棉花纤维细胞壁物质的合成和运输是通过由内质网、高尔基复合体和细胞质膜组成的完整的膜系统来完成的。
短句来源
     The comparison analysis show that the method by using a extraction buffer with high ion density and pH value was better.
     研究表明 ,用高离子、高 p H值提取液提取棉花纤维细胞的 RNA比其它两种提取液TRIZOL提取液和异硫氢酸胍提取液更适合。
短句来源
     By a combination of two rounds of reverse Northern hybridization and Northern blot analyses, a number of such cDNA fragments were proved to represent fiber-specific/preferential genes. Sequencing determination and database searching indicated that most of these genes are novel.
     在此基础上 ,结合两轮反Northern杂交筛选和Northern杂交分析 ,获得了多个仅在棉花纤维细胞中特异表达或在纤维中优先表达基因的cDNA片段 ,序列测定和数据库搜索分析表明 ,这些cDNA片段中的多数还未有报道。
短句来源
  相似匹配句对
     THE STUDY ON MORPHOLOGY OF COTTON OVULE AND FIBER DIFFERENTIATION
     棉花胚珠的形态学特征与纤维细胞的分化
短句来源
     Initiation of Cotton Fiber Cells and the Effects of Temperature and Plant Growth Substances on It
     棉花纤维细胞起始及温度、植物生长物质对其影响
短句来源
     Usage of mean in cotton
     平均数在棉花上的应用
短句来源
     Gossypium Speciation via Polyploidy
     棉花的多倍体起源
短句来源
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  cotton fiber cell
Atomic force microscopy of cotton fiber cell wall surfaces in air and water: quantitative and qualitative aspects
例句来源      
This work was undertaken in order to extend studies concerning nutritional effects and regulation of nucleotide sugar incorporation into β-1,3- and β-1,4-glucan components of cotton fiber cell walls.
例句来源      
However, the role of the actin cytoskeleton in cotton fiber cell development remains largely unknown.
例句来源      
In this work, we found a prominent role for the plant hormone ethylene in promoting cotton fiber cell elongation.
例句来源      
  cotton fiber cells
The results showed that GhTub1 gene was specifically expressed in cotton fiber cells.
例句来源      
We report here the cloning and characterization of a receptor-like kinase gene (designated GhRLK1), expression of which is induced during the period of active secondary wall synthesis in the cotton fiber cells.
例句来源      
As a step towards characterizing protein components of the plant cytoskeleton, we have isolated cytoskeletons from cytoplasts (anucleate protoplasts) prepared from cotton fiber cells grown in ovule culture.
例句来源      
Optical microphotos illustrate the formation process of the fiber cell wall at different stages of growth and noncontractile motion in cotton fiber cells.
例句来源      
This result further confirmed that the GhACT1 gene is preferentially expressed, especially in elongation phase in cotton fiber cells.
例句来源      
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         The cotton fiber cells contain a lot of endogenous phenolics, polysaccharides and secondary metabolites that may result in difficulty of RNA isolation from fiber cells. In order to isolate high quality RNA from cotton fiber cells the comparison of three methods of RNA isolation with each other was conducted. The comparison analysis show that the method by using a extraction buffer with high ion density and pH value was better. This extraction buffer with high ion density and ph value was characterized by...
            棉花纤维细胞含有高的棉酚、多糖和次生代谢物质 ,对其 RNA的提取造成困难。为高质量提取棉花纤维细胞的 RNA,进行了三种提取方法的比较研究。研究表明 ,用高离子、高 p H值提取液提取棉花纤维细胞的 RNA比其它两种提取液TRIZOL提取液和异硫氢酸胍提取液更适合。高离子、高 p H值提取液的特点是 :p H值偏碱性 ,加入脱氧胆酸钠及 NP- 40两类盐类 ,提高其他盐类的浓度 ,并加入 PVP和巯基乙醇两种抗氧化剂。用本方法提取的棉花纤维等组织细胞的 RNA,纯度高 ,不降解 ;如棉纤维细胞 RNA的 A2 6 0 /A2 80 可达 1 .85 ,平均得率为 0 .65 mg· g- 1,棉叶 RNA的A2 6 0 /A2 80 可达 2 .0 2 ,平均得率为 6.35 mg· g- 1,适合于 Northern杂交及 RT- PCR等分子生物学实验研究
文摘来源
         LIM domain protein plays an important role in various cellular processes,including construction of cytoskeleton,transcription control and signal transduction.Based on cotton fiber EST database and contig analysis,the coding region of a cotton LIM domain protein gene ( GhLIM1 ) was obtained by RT PCR from 4DPA (day post anthesis) ovule with fiber.The cloned fragment of 848 bp contains an open reading frame of 570 bp,coding for a polypeptide of 189 amino acids.It was demonstrated that the deduced GhLIM1 ...
            LIM结构域蛋白是一个重要的发育调控因子 ,参与基因转录、细胞骨架建成和信号传导等许多发育调控过程。胞质骨架是形成和稳定细胞形态以及传递物质、能量和信息的重要成分。为研究棉花纤维细胞发育过程中胞质骨架的形成和作用机理 ,通过棉花纤维EST序列整合 ,从陆地棉徐州 14 2胚珠 (含纤维 )中扩增并克隆出棉花LIM结构域基因的编码区段。该棉花LIM结构域基因 (GhLIM1)长 84 8bp ,包含一个 5 70bp的开放阅读框 ,推导的氨基酸序列 (189个氨基酸 )与拟南芥、烟草和向日葵的LIM结构域蛋白有极高的同源性 ,而且两个LIM结构域完整。每个LIM结构域具有植物LIM结构域共有的双锌指结构C X2 C X17~ 19 H X2 C X2 C X2 C X16~ 2 4 C X2 H。RT PCR和Northern杂交分析表明 ,该基因 (GhLIM1)在陆地棉的根、茎尖、下胚轴、叶片、花蕾、花药、胚珠和不同发育时期的陆地棉纤维 (4DPA、12DPA、18DPA)以及海岛棉纤维 ...
文摘来源
         Fluorescence differential display (FDD) technique was used to identify genes that are specifically or preferentially expressed in different developmental stages of cotton fiber cells. One hundred and nine differentially displayed cDNA fragments were isolated using 9, 21 and 27 DPA (days postanthesis) fibers as experimental materials. By a combination of two rounds of reverse Northern hybridization and Northern blot analyses, a number of such cDNA fragments were proved to represent fiber-specific/preferentia...
            以陆地棉 (GossypiumhirsutumL .)品种TM 1开花后 9d、2 1d、2 7d三个不同发育时期的棉花纤维为材料 ,利用mRNA荧光差异显示 (FDD)技术 ,筛选到 10 9个差异显示的cDNA片段。在此基础上 ,结合两轮反Northern杂交筛选和Northern杂交分析 ,获得了多个仅在棉花纤维细胞中特异表达或在纤维中优先表达基因的cDNA片段 ,序列测定和数据库搜索分析表明 ,这些cDNA片段中的多数还未有报道。本工作为克隆上述基因的全长cDNA ,并进一步研究它们在棉纤维发育中的功能奠定了基础。
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