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改良浸种法
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  improved seed-soaking method
     Genetic and Breeding Effects of Introduction of Exogenous DNA into Soybeans by Improved Seed-Soaking Method
     应用改良浸种法将外源DNA导入大豆的遗传育种效应研究
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  “改良浸种法”译为未确定词的双语例句
     In this method, the sterilized TPSs were immersed in 0. 2~0. 3% colchicine solution for 12~48 hrs and then culturing them on MS medium in test tube. Using the tissue culture method after colchicine treatment could overcome the disadvantage of inhibiting root growth by colchicine, so that the doubling frequency increased with the increase of seedling su- rvival frequency. The maximum doubling frequency was 70. 96%.
     试验表明:在无菌条件下,用0.2~0.3%的秋水仙素浸种12~48小时后,再用组织培养的方法在无菌条件下培养处理实生苗的改良浸种法,与常规方法相比能有效克服秋水仙素对根的毒害作用,提高处理苗的成活率,从而大大提高加倍频率(加倍频率最高达70.96%);
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  相似匹配句对
     In breeding of polyploid of Brassica oleracea L.var.
     用改良L.
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     The improvement A.
     改良 A .
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     soaked in cold water for 48 hours;
     冷水浸种48h;
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     An Improved Synthesis of Acyclovir
     阿昔洛韦的改良合成
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     Genetic and Breeding Effects of Introduction of Exogenous DNA into Soybeans by Improved Seed-Soaking Method
     应用改良浸种将外源DNA导入大豆的遗传育种效应研究
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In this paper, the improved procedures of doubling chromosome number are presented. The results showed that the improved method of immersing true potato seeds (TPSs) could increase the doubling frequency greatly. In this method, the sterilized TPSs were immersed in 0. 2~0. 3% colchicine solution for 12~48 hrs and then culturing them on MS medium in test tube. Using the tissue culture method after colchicine treatment could overcome the disadvantage of inhibiting root growth by colchicine, so that the doubling...

In this paper, the improved procedures of doubling chromosome number are presented. The results showed that the improved method of immersing true potato seeds (TPSs) could increase the doubling frequency greatly. In this method, the sterilized TPSs were immersed in 0. 2~0. 3% colchicine solution for 12~48 hrs and then culturing them on MS medium in test tube. Using the tissue culture method after colchicine treatment could overcome the disadvantage of inhibiting root growth by colchicine, so that the doubling frequency increased with the increase of seedling su- rvival frequency. The maximum doubling frequency was 70. 96%. For the dihaploid and monohaploid clones, the method of immersing stem segments of in vitro cultured plantlets in 0. 2~0. 5% colchicine solution and then cu- lturing them again could increase the doubling frequency obviously. The maximum frequency was 70%. The conventional method of immersing tuber sprout tips with colchicine solution or paste was ineffective, especially in the arid area.

本文报道了用双单倍体的实生籽和一单倍体无性系试管苗,按常规方法和改良方法对染色体加倍的试验结果。试验表明:在无菌条件下,用0.2~0.3%的秋水仙素浸种12~48小时后,再用组织培养的方法在无菌条件下培养处理实生苗的改良浸种法,与常规方法相比能有效克服秋水仙素对根的毒害作用,提高处理苗的成活率,从而大大提高加倍频率(加倍频率最高达70.96%);对一单倍体无性系,采用秋水仙素浸试管苗切段后再用组织培养使之分化苗的改良方法也能有效地提高加倍频率(最高达70%)。而常规的幼芽处理法在干旱地区是无效的。

Maize DNA is introduced into soybean through“improved seed-soakingmethod”.The progeny with exogeneous DNA has heritably variations in bear stage,plantheight,number of pods per plant,seed weight per plant,100-seed weight,number of nodes,colour of hilum and so on.

用玉米作DNA供体,栽培大豆品种作为受体,采用“改良浸种法”进行外源DNA导入,其后代在生育期、株高、结荚数、单株产量、百粒重、节数、种脐色等性状上产生了明显的变异.

The improved seed-soaking method was used to introduce the exogenous DNA of maize and America Ginseng (Panax quinquefolium) into soybean and the biological, genetica and breeding effects of the introduction in this experiment were discussed. The results indicated that the method could not only reduce the germinating and seedling percentage of the soaking seeds and effect the plant's development, but also result in the heritable variations of many agronomic characteristics. The variation characteristics included...

The improved seed-soaking method was used to introduce the exogenous DNA of maize and America Ginseng (Panax quinquefolium) into soybean and the biological, genetica and breeding effects of the introduction in this experiment were discussed. The results indicated that the method could not only reduce the germinating and seedling percentage of the soaking seeds and effect the plant's development, but also result in the heritable variations of many agronomic characteristics. The variation characteristics included growth period, plant height, pod number per plant, seed weight per plant, 100-seed weight, node number, hilum color, protein content and isoflavone content etc. Many variable traits, such as early-maturity and short-height, could rapidly and stably inherit and could be used as materials for breeding selection. The variation frequency of introducing was 7.6~18.9% in D2,and 21.5~26.4% in D3. The frequency seemed to rise with the increase of the generations. The variation characteristics of the traits were further explored and the selection methods of progeny with exogenous DNA were brought out for improving the breeding efficiency.

应用"改良浸种法"将玉米、西洋参DNA导入大豆,结果表明,该导入方法可降低导入当代种子发芽率、出苗率,导入后代在生育期、株高、单株结荚数、百粒质量、节数、种脐色、蛋白质和异黄酮含量等性状上产生广泛变异,且许多变异性状(如早熟性、株高)在早世代就能迅速稳定遗传。导入引起的变异频率在D2代为7 6%~18 9%,D3代为21 5%~26 4%,随世代的增加变异频率明显增加。并进一步探讨了这些性状的变异特点。同时,为了提高育种效率,提出了导入后代的选择方法。

 
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