The optimal medium composition for the lipase production is(%):soybean flour 1.0,corn milk 1.0,glucose 1.0, K2HPO4 0.5,NaNO3 0.5. Culture medium volumes for 50 mL flask were cultivated aerobically at 20℃ with an aeration rate of 180 r/min for 40 hours.
The optimal culture conditions for the FS132 producing lipase obtained were as follows: the optimal culture temperature of 36℃,initiation pH of 7.0 for the culture medium,oxygen-consumption of 25mL medium for each 250mL flask and culture time of 45 hours.
Under the condition of shaking flask fermentation,by means of orthogonal test,the effects of nutrition factors,such as glucose,soybean powder,glycerol and 95% ethanol,on the production of phenazine-1-carboxylic acid(PCA) by a genetic engineering strain M18G. The optimized components of culture medium were 6g glucose,22g soybean powder,6mL glycerol,5.9mL 95% ethanol in 1 liter;
The maximum yield of β-carotene(13.43mg/L) is obtained with the optimum medium at yeast extract 4.23g/L,glucose 12.11g/L,pH6.7,volume of inoculums 30mL/L,tomato exact 2.5mL/L,volume of media 40mL/250mL,peanut oil 0.5mL /L and(NH4)2SO4 5mL/L.
The optimum formula of the media,which are determined to use the uniform design test,are as followed:starch 3 g,bran 14 g,yeast extract 6 g,KH_2PO_4 0.5 g,MgSO_4·7H_2O 0.4 g and sea water 1000 mL.
In the media composed of 1.2% chitin,0.2% yeast extract and 0.2%peptone,and initial pH 6.8,the strainwas incubated at 28℃ for 96 h,chitinase activity reached 11.69 U/mL.
For the protoplast isolation of bacteria 80, the results showed that the second passage bacteria 80 was better than that of other passages, and was selected to be cultured in culture media with 1% Gly for 16 h.
For the protoplast isolation of bacteria 80, the results showed that the second passage bacteria 80 was better than that of other passages, and was selected to be cultured in culture media with 1% Gly for 16 h.
With the single factor test and relationship test, the optimal culture media for AT-22 strain producing agarase is decided as: agar 0.3%, (NH4)2SO4 0.5%, peptone 0.3 %, NaCl 3.0%, KH2PO4 0.1 %, MgCl2 0.1 %;
In this paper, Aeromonas hydrophila 4AK4 was chosen as the host strain to study the PHBHHx synthesis and production. It was found that addition of 4 g L-1 butanol to the culture media could decrease 3HHx fraction from 14.4 mol% to 5.8 mol%.
本文在嗜水性气单孢菌Aeromonas hydrophila 4AK4发酵生产PHBHHx的过程中发现，培养基中添加4 g L-1的丁醇能使PHBHHx中3HHx单体的组成比例由原来的14.4 mol%降低为5.8 mol%。
The objective of this paper is to deal with a kind of fuzzy linear programming problem based on interval-valued fuzzy sets (IVFLP) through the medium of procedure that turns IVFLP into parametric linear programming via the mathematical programming.
The conclusion that the equilibrium is the continuous function of the dilution rate and substrate concentration in medium in a certain range is concluded.
Many food algae and red tide algae were cultivated in the f/2 medium, and the nitric oxide (NO) concentration of the medium and the cell density were determined.
Time courses of the content of extracellular polymers (ECPs) in culture media of morphogenic and non-morphogenic calluses (MCs and NCs, respectively) of tartar buckwheat Fagopyrum tataricum (L.) Gaertn.
An endophyte (designated ZP5SE) was isolated from the seed of Nothapodytes foetida and was examined as a potential source of anticancer drug lead compound, i.e., camptothecin, when grown in Sabouraud liquid culture media under shake flask conditions.
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